FastQCFastQC Report
Fri 10 Feb 2017
ERR1632121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1374830
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG20660.15027312467723283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20080.14605442127390295No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA19660.14299949811976753No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG19410.14118109148040123No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT19090.13885353098201234No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC18340.13339831106391337No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC18270.13288915720489078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.11623255238829527No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC15600.11346857429645847No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG14670.10670410159801577No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT14350.10437654109962685No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC13760.10008510143072234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7350.028.6938781
CGACACT502.7024758E-422.24
TAAGCGG502.7024758E-422.25
AACCGCG806.962164E-720.81257
ATAAGCG609.238073E-418.54
CTTATAC8950.017.56983237
TCGATTG650.001580147117.0769238
GTATCAA12450.016.7911642
TCTTATA15350.016.27035737
TCTAGCG700.0025928415.85714328
GTATAAT1201.936074E-615.4166661
TACACTC1201.936074E-615.4166665
CTAGCGG855.365103E-415.23529329
TACGCCA750.00410495114.815
TAATCTA1405.9967897E-714.53571520
CTCTTAT25850.014.02707937
GTACAAT800.006299447713.8750011
TTCGGAA1102.4569084E-413.45454531
GTATTAA4050.013.2469141
TTAAGGC1409.561296E-613.2142863
Produced by FastQC (version 0.11.5)