##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632121.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1374830
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55788424750696	34.0	31.0	34.0	31.0	34.0
2	32.7253842293229	34.0	31.0	34.0	31.0	34.0
3	32.725982848788576	34.0	31.0	34.0	31.0	34.0
4	36.248179774954	37.0	37.0	37.0	35.0	37.0
5	36.12532094877185	37.0	35.0	37.0	35.0	37.0
6	36.159688834255874	37.0	36.0	37.0	35.0	37.0
7	36.1551108137006	37.0	36.0	37.0	35.0	37.0
8	36.13236036455416	37.0	35.0	37.0	35.0	37.0
9	37.86136613254002	39.0	38.0	39.0	35.0	39.0
10	37.78906846664678	39.0	37.0	39.0	35.0	39.0
11	37.89513103438243	39.0	38.0	39.0	35.0	39.0
12	37.87284246052239	39.0	38.0	39.0	35.0	39.0
13	37.8890386447779	39.0	38.0	39.0	35.0	39.0
14	39.2527687059491	40.0	39.0	41.0	36.0	41.0
15	39.27407315813591	40.0	39.0	41.0	36.0	41.0
16	39.15314329771681	40.0	39.0	41.0	36.0	41.0
17	39.207516565684486	40.0	39.0	41.0	36.0	41.0
18	39.216130721616494	40.0	39.0	41.0	36.0	41.0
19	39.28303572078002	40.0	39.0	41.0	36.0	41.0
20	39.26074569219467	40.0	39.0	41.0	36.0	41.0
21	39.23274804884968	40.0	39.0	41.0	36.0	41.0
22	39.22274244815723	40.0	39.0	41.0	36.0	41.0
23	39.189373231599546	40.0	39.0	41.0	36.0	41.0
24	39.13755009710292	40.0	39.0	41.0	36.0	41.0
25	39.082661129012315	40.0	39.0	41.0	36.0	41.0
26	38.99175825374773	40.0	39.0	41.0	36.0	41.0
27	38.923978964671996	40.0	38.0	41.0	35.0	41.0
28	38.87111715630296	40.0	38.0	41.0	35.0	41.0
29	38.74394288748427	40.0	38.0	41.0	35.0	41.0
30	38.710007782780416	40.0	38.0	41.0	35.0	41.0
31	38.63638777157903	40.0	38.0	41.0	35.0	41.0
32	38.566525315857234	40.0	38.0	41.0	35.0	41.0
33	38.44418655397394	40.0	38.0	41.0	34.0	41.0
34	38.45038222907559	40.0	38.0	41.0	34.0	41.0
35	38.4341496766873	40.0	38.0	41.0	34.0	41.0
36	38.408070816028165	40.0	38.0	41.0	34.0	41.0
37	38.38198540910513	40.0	38.0	41.0	34.0	41.0
38	38.310001963879174	40.0	38.0	41.0	34.0	41.0
39	38.13199668322629	40.0	38.0	41.0	34.0	41.0
40	38.15592400514973	40.0	38.0	41.0	34.0	41.0
41	38.16302451939512	40.0	38.0	41.0	34.0	41.0
42	38.049283911465416	40.0	38.0	41.0	34.0	41.0
43	37.239423055941465	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	4.0
16	2.0
17	8.0
18	24.0
19	53.0
20	100.0
21	175.0
22	387.0
23	607.0
24	1006.0
25	1592.0
26	2323.0
27	3581.0
28	5176.0
29	7467.0
30	10484.0
31	14163.0
32	19312.0
33	26013.0
34	37535.0
35	55042.0
36	87355.0
37	160723.0
38	385874.0
39	555823.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.0417069746805	18.771048056850663	13.107875155473767	26.07936981299506
2	19.101197966294016	20.646043510834065	34.833834001294704	25.418924521577214
3	20.62764123564368	21.990427907450375	30.23370162129136	27.148229235614586
4	16.063076889506338	15.911858193376634	33.08663616592597	34.93842875119106
5	15.865452455940007	35.05116996283177	33.456136395045206	15.627241186183019
6	34.92773652015158	33.41787711935294	15.619094724438659	16.035291636056822
7	28.676854592931488	29.867401787857407	21.063913356560448	20.391830262650654
8	26.005324294640065	33.660598037575554	20.337132590938516	19.996945076845865
9	25.89520158856004	15.067826567648362	20.16322017994952	38.87375166384208
10	17.534677014612715	27.106405882909158	33.050340769404215	22.30857633307391
11	35.34757024504847	21.59416073259967	21.579104325625714	21.47916469672614
12	21.378424968905247	25.4424910716234	28.36452506855393	24.814558890917425
13	30.03745917677095	19.89824196446106	24.655120996777782	25.409177861990212
14	23.132532749503575	21.396681771564484	24.01736942021923	31.453416058712712
15	25.787624651775126	27.68800506244408	22.150811373042487	24.373558912738304
16	25.48358706167308	26.269502411207203	23.552584683197196	24.69432584392252
17	24.156513896263537	26.478110020875306	24.92584537724664	24.439530705614512
18	23.28891572048908	25.43514470880036	26.403700821192437	24.87223874951812
19	24.88874988180357	25.530356480437582	26.229933882734592	23.35095975502426
20	24.911152651600563	24.90220609093488	26.537899231177676	23.648742026286886
21	24.56078206032746	25.792643454099778	25.097575700268397	24.548998785304367
22	24.928682091604053	24.927372838823707	25.185150164020282	24.958794905551958
23	24.363521308089002	25.571888888080707	25.334041299651595	24.7305485041787
24	25.714379232341457	25.398267422154014	24.93930158637795	23.94805175912658
25	25.267633089181935	24.745459438621502	25.36153560803881	24.62537186415775
26	25.021129885149435	25.15561924019697	25.166747888829892	24.656502985823703
27	24.748223416713337	24.905479222885738	25.200642988587678	25.145654371813244
28	24.18189885294909	25.164493064597078	25.8565786315399	24.797029450913932
29	24.279801866412576	25.76056676098136	25.55959645919859	24.400034913407474
30	24.05650153109839	26.265574652866174	25.388738971363733	24.289184844671706
31	24.816304561291215	25.049424292457978	25.823338158172284	24.310932988078527
32	23.906301142686733	25.06331691918274	25.87941781893034	25.150964119200193
33	24.330353570986958	24.769535142526713	25.80195369609333	25.098157590392994
34	24.563691510950445	24.7093095146309	25.607675130743434	25.119323843675218
35	24.517140300982668	24.564055192278317	25.83512143319538	25.08368307354364
36	24.104871147705534	24.71731050384411	26.212477178996675	24.965341169453676
37	24.924972542059745	23.01099044972833	26.441305470494537	25.622731537717392
38	23.630485223627648	23.839601987154776	27.795872944291293	24.73403984492628
39	23.49439567073747	23.42245950408414	28.252802164631262	24.83034266054712
40	23.124095342696915	22.757140882872793	29.050937206781928	25.067826567648368
41	22.43819235832794	23.506615363354015	29.1089807467105	24.946211531607545
42	22.87482816057258	22.948000843740683	28.687983241564414	25.489187754122327
43	21.881759926681845	23.383109184408255	29.073049031516625	25.662081857393275
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	73.0
2	94.0
3	228.0
4	362.0
5	362.0
6	497.0
7	632.0
8	585.5
9	539.0
10	843.0
11	1147.0
12	1147.0
13	1963.0
14	2779.0
15	4084.5
16	5390.0
17	5625.0
18	5860.0
19	5860.0
20	7359.5
21	8859.0
22	10408.5
23	11958.0
24	13957.5
25	15957.0
26	15957.0
27	17590.5
28	19224.0
29	26039.0
30	32854.0
31	35093.5
32	37333.0
33	37333.0
34	42028.5
35	46724.0
36	50851.5
37	54979.0
38	64184.5
39	73390.0
40	73390.0
41	80398.5
42	87407.0
43	84068.5
44	80730.0
45	86136.0
46	91542.0
47	91542.0
48	96539.0
49	101536.0
50	107953.0
51	114370.0
52	119449.0
53	124528.0
54	124528.0
55	108715.5
56	92903.0
57	86333.0
58	79763.0
59	72964.0
60	66165.0
61	66165.0
62	61480.0
63	56795.0
64	51324.0
65	45853.0
66	40489.0
67	35125.0
68	35125.0
69	30813.0
70	26501.0
71	23030.0
72	19559.0
73	16243.0
74	12927.0
75	12927.0
76	10467.5
77	8008.0
78	6779.5
79	5551.0
80	4418.5
81	3286.0
82	3286.0
83	2656.5
84	2027.0
85	1536.0
86	1045.0
87	835.0
88	625.0
89	625.0
90	471.5
91	318.0
92	207.5
93	97.0
94	61.0
95	25.0
96	25.0
97	16.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1374830.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.38679015290256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.63347183353561	47.983499246614315
2	7.788692333882562	8.627813357245486
3	2.0714357698728847	3.441905349034969
4	0.949400535681402	2.1033699296335637
5	0.530477483210139	1.469072252169993
6	0.3392300498985868	1.1273318152375016
7	0.25990585253742404	1.0076745639801081
8	0.18429175229913586	0.8165862889202349
9	0.13727456522804884	0.6842877783855188
>10	0.8773601093326842	9.37374798835377
>50	0.11032661678965061	4.273381775942841
>100	0.10400993808203428	12.22958049592491
>500	0.01029538740836466	3.8946603949814134
>1k	0.003827772241571476	2.967088763575396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2066	0.15027312467723283	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2008	0.14605442127390295	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1966	0.14299949811976753	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1941	0.14118109148040123	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1909	0.13885353098201234	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1834	0.13339831106391337	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	1827	0.13288915720489078	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1598	0.11623255238829527	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	1560	0.11346857429645847	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1467	0.10670410159801577	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	1435	0.10437654109962685	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	1376	0.10008510143072234	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4547253114930572E-4	0.0
2	0.0	0.0	0.0	2.182087967239586E-4	0.0
3	0.0	0.0	0.0	2.9094506229861145E-4	0.0
4	0.0	0.0	0.0	4.364175934479172E-4	0.0
5	7.273626557465286E-5	7.273626557465286E-5	0.0	4.364175934479172E-4	0.0
6	7.273626557465286E-5	7.273626557465286E-5	0.0	4.364175934479172E-4	0.0
7	7.273626557465286E-5	7.273626557465286E-5	0.0	4.364175934479172E-4	0.0
8	7.273626557465286E-5	7.273626557465286E-5	0.0	4.364175934479172E-4	0.0
9	7.273626557465286E-5	7.273626557465286E-5	0.0	5.818901245972229E-4	0.0
10	7.273626557465286E-5	7.273626557465286E-5	0.0	7.273626557465286E-4	0.0
11	7.273626557465286E-5	7.273626557465286E-5	0.0	7.273626557465286E-4	0.0
12	7.273626557465286E-5	7.273626557465286E-5	0.0	7.273626557465286E-4	0.0
13	7.273626557465286E-5	7.273626557465286E-5	0.0	7.273626557465286E-4	0.0
14	7.273626557465286E-5	7.273626557465286E-5	0.0	7.273626557465286E-4	0.0
15	7.273626557465286E-5	7.273626557465286E-5	0.0	7.273626557465286E-4	0.0
16	7.273626557465286E-5	7.273626557465286E-5	0.0	8.728351868958344E-4	0.0
17	7.273626557465286E-5	7.273626557465286E-5	0.0	0.00101830771804514	0.0
18	7.273626557465286E-5	1.4547253114930572E-4	0.0	0.00101830771804514	0.0
19	7.273626557465286E-5	2.182087967239586E-4	0.0	0.00101830771804514	0.0
20	7.273626557465286E-5	2.182087967239586E-4	0.0	0.001091043983619793	0.0
21	7.273626557465286E-5	2.182087967239586E-4	0.0	0.0012365165147690987	0.0
22	7.273626557465286E-5	2.182087967239586E-4	0.0	0.0017456703737916688	0.0
23	7.273626557465286E-5	3.636813278732643E-4	0.0	0.0023275604983888916	0.0
24	7.273626557465286E-5	4.364175934479172E-4	0.0	0.004655120996777783	0.0
25	7.273626557465286E-5	4.364175934479172E-4	0.0	0.006619000167293411	0.0
26	7.273626557465286E-5	4.364175934479172E-4	0.0	0.0114195936952205	0.0
27	7.273626557465286E-5	4.364175934479172E-4	0.0	0.034186044820086844	7.273626557465286E-5
28	7.273626557465286E-5	4.364175934479172E-4	0.0	0.09281147487325705	7.273626557465286E-5
29	7.273626557465286E-5	4.364175934479172E-4	0.0	0.16656604816595508	7.273626557465286E-5
30	7.273626557465286E-5	4.364175934479172E-4	0.0	0.2676694573147225	7.273626557465286E-5
31	7.273626557465286E-5	4.364175934479172E-4	0.0	0.5623968054232159	7.273626557465286E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	735	0.0	28.693878	1
CGACACT	50	2.7024758E-4	22.2	4
TAAGCGG	50	2.7024758E-4	22.2	5
AACCGCG	80	6.962164E-7	20.8125	7
ATAAGCG	60	9.238073E-4	18.5	4
CTTATAC	895	0.0	17.569832	37
TCGATTG	65	0.0015801471	17.076923	8
GTATCAA	1245	0.0	16.791164	2
TCTTATA	1535	0.0	16.270357	37
TCTAGCG	70	0.00259284	15.857143	28
GTATAAT	120	1.936074E-6	15.416666	1
TACACTC	120	1.936074E-6	15.416666	5
CTAGCGG	85	5.365103E-4	15.235293	29
TACGCCA	75	0.004104951	14.8	15
TAATCTA	140	5.9967897E-7	14.535715	20
CTCTTAT	2585	0.0	14.027079	37
GTACAAT	80	0.0062994477	13.875001	1
TTCGGAA	110	2.4569084E-4	13.454545	31
GTATTAA	405	0.0	13.246914	1
TTAAGGC	140	9.561296E-6	13.214286	3
>>END_MODULE