FastQCFastQC Report
Fri 10 Feb 2017
ERR1632120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661776
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24970.15026092566025745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.13840613897420592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG451.3235591E-424.6666661
GGTATCA13600.019.8602941
TATACGG650.001580334917.0769232
CTTATAC11200.016.187537
TCTTATA19350.015.5839837
AATACTG2800.014.5357145
GGTTAAG1304.446545E-614.2307691
GTATCAA19300.013.9948182
CGCTAAC800.0063001813.87511
TCGTTCG950.00124548213.6315799
TACGCTA950.00124548213.6315799
GTTCGCG950.00124548213.63157930
TATACGC950.00124548213.6315797
TATCGGA950.00124548213.63157917
GTTATCG950.00124548213.63157915
TAAGACT2457.2759576E-1213.5918364
CGAATTA1501.3063836E-613.56666715
CTCTTAT30300.013.55445437
GTATAGA1805.16211E-813.3611111
TTATGAC1307.001482E-512.8076933