##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632120.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1661776
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96817019863086	33.0	31.0	34.0	30.0	34.0
2	32.14758788188059	33.0	31.0	34.0	30.0	34.0
3	32.16707847507727	34.0	31.0	34.0	30.0	34.0
4	35.88245648029578	37.0	35.0	37.0	35.0	37.0
5	35.50017872444902	37.0	35.0	37.0	33.0	37.0
6	35.48724075928404	37.0	35.0	37.0	33.0	37.0
7	35.480177232069785	37.0	35.0	37.0	33.0	37.0
8	35.43495152174541	37.0	35.0	37.0	33.0	37.0
9	37.10775459508381	39.0	37.0	39.0	33.0	39.0
10	36.91016659285006	39.0	37.0	39.0	33.0	39.0
11	37.06076510913625	39.0	37.0	39.0	33.0	39.0
12	37.00051450977749	39.0	37.0	39.0	33.0	39.0
13	37.065797676702516	39.0	37.0	39.0	33.0	39.0
14	38.22350605617123	40.0	38.0	41.0	34.0	41.0
15	38.20345461722879	40.0	38.0	41.0	34.0	41.0
16	38.07388841817429	40.0	38.0	41.0	33.0	41.0
17	38.111710001829366	40.0	38.0	41.0	33.0	41.0
18	38.11988137992124	40.0	38.0	41.0	33.0	41.0
19	38.17703168176698	40.0	38.0	41.0	34.0	41.0
20	38.13861796054342	40.0	38.0	41.0	34.0	41.0
21	38.107939938956875	40.0	38.0	41.0	34.0	41.0
22	38.08083520281915	40.0	38.0	41.0	34.0	41.0
23	38.02412358825738	40.0	37.0	41.0	33.0	41.0
24	37.95175523054852	40.0	37.0	41.0	33.0	41.0
25	37.86361218359153	39.0	37.0	41.0	33.0	41.0
26	37.736061298273654	39.0	37.0	40.0	33.0	41.0
27	37.63231205649859	39.0	37.0	40.0	33.0	41.0
28	37.54007640018872	39.0	37.0	40.0	32.0	41.0
29	37.37675414737004	39.0	37.0	40.0	32.0	41.0
30	37.30744396356669	39.0	36.0	40.0	32.0	41.0
31	37.20186715899134	39.0	36.0	40.0	31.0	41.0
32	37.102525250093876	39.0	36.0	40.0	31.0	41.0
33	36.941446982024054	39.0	36.0	40.0	31.0	41.0
34	36.93197879858657	39.0	36.0	40.0	31.0	41.0
35	36.88599486332695	39.0	36.0	40.0	31.0	41.0
36	36.84647991064981	39.0	36.0	40.0	31.0	41.0
37	36.81160096186249	39.0	36.0	40.0	31.0	41.0
38	36.72564894426204	39.0	35.0	40.0	31.0	41.0
39	36.51787364843396	39.0	35.0	40.0	30.0	41.0
40	36.50987136653797	38.0	35.0	40.0	30.0	41.0
41	36.489209737052406	38.0	35.0	40.0	30.0	41.0
42	36.367544723235866	38.0	35.0	40.0	30.0	41.0
43	35.409185714560806	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	1.0
14	0.0
15	7.0
16	19.0
17	38.0
18	102.0
19	185.0
20	341.0
21	602.0
22	1063.0
23	1777.0
24	2861.0
25	4409.0
26	6488.0
27	9627.0
28	13190.0
29	18342.0
30	25129.0
31	33447.0
32	44982.0
33	61692.0
34	87676.0
35	127024.0
36	198262.0
37	318947.0
38	464051.0
39	241510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.658517152732976	18.19535244220641	12.400106873609921	26.746023531450692
2	20.455825574566006	20.172092989668887	32.67552305485216	26.69655838091295
3	21.3940988436468	21.265802370475924	28.71271458969199	28.627384196185286
4	16.503367481537826	15.604630226937926	32.24676490694293	35.645237384581314
5	16.962815686350023	33.81105516026227	32.55324423989755	16.672884913490147
6	36.18026737658987	32.68352654027979	15.040534945744794	16.095671137385544
7	30.547318050086176	28.501494786300924	20.379220785472892	20.571966378140015
8	27.78033862566315	31.761260242054284	19.487343661239542	20.97105747104303
9	27.04636485302472	13.866309298004062	19.21727116049335	39.87005468847787
10	18.30487382174252	25.543394536929164	32.144344364102025	24.007387277226293
11	36.97772744340994	20.590079529370986	20.319946852042634	22.112246175176438
12	22.69680149430489	24.141219995956135	27.891725479246297	25.27025303049268
13	31.77473979645873	18.667858965347918	23.198313130048813	26.35908810814454
14	24.017256236700977	19.8878188155323	23.742851022039073	32.35207392572765
15	26.715995416951504	25.944591810207875	21.00541829945793	26.333994473382695
16	26.289223096253643	24.763686561847084	23.019347974696952	25.927742367202317
17	24.969550649425678	25.137563666823926	24.432354300459266	25.46053138329113
18	25.222894060330635	24.045117994242304	25.032555530950017	25.69943241447704
19	25.906319503952396	24.1614393275628	25.07702602516825	24.85521514331655
20	26.227903159029857	23.80994791115048	24.708143576510913	25.25400535330875
21	26.277789545642733	23.888598704037125	24.21030271227891	25.62330903804123
22	26.52156487998382	23.951723938725795	24.081103590375598	25.445607590914783
23	26.097199622572475	23.818192102906767	24.232447694514782	25.852160580005968
24	25.85799770847575	24.144830590885896	24.168901223750975	25.828270476887376
25	26.153645256641088	23.572792000847286	24.17100740412667	26.10255533838496
26	25.824840411704102	24.22191679263631	24.206571764184822	25.74667103147476
27	25.82429882246464	23.934092200152126	24.10818305234881	26.13342592503442
28	25.590272094433907	24.050714416383435	24.576838274231907	25.782175214950755
29	25.320380123434205	24.107761816273673	24.925441214700417	25.646416845591702
30	25.50452046485206	24.507153792087504	24.53706155342236	25.451264189638074
31	25.86190918631633	24.025440251875104	24.366701649319765	25.74594891248881
32	25.324893367096408	24.000767853188396	24.67414380758899	26.000194972126206
33	25.192264180009822	23.970198149449747	24.890779503374702	25.94675816716573
34	25.921363649493074	23.453221132090004	24.514374981947025	26.111040236469897
35	25.371650571436827	23.50196416364179	25.272479564032697	25.853905700888685
36	25.12853717949952	23.353087299371275	25.225120593870653	26.29325492725855
37	25.286982120333906	22.4004318271536	25.98797912594718	26.324606926565313
38	24.62191053427177	22.63638420581354	26.584750291254654	26.156954968660035
39	24.375186547404702	22.470236662462327	27.098598126341937	26.05597866379103
40	23.979946755760103	22.28272643244336	27.864946900183902	25.872379911612637
41	23.50569511173588	22.239278940121892	28.144286594583146	26.110739353559083
42	23.443231819450997	22.508448792135642	27.91579611211138	26.132523276301985
43	23.008034777250362	22.375097486063105	28.25603450765928	26.360833229027257
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	86.0
1	83.5
2	81.0
3	215.0
4	349.0
5	349.0
6	496.5
7	644.0
8	668.0
9	692.0
10	987.5
11	1283.0
12	1283.0
13	2249.5
14	3216.0
15	4521.5
16	5827.0
17	5747.5
18	5668.0
19	5668.0
20	7536.5
21	9405.0
22	9820.0
23	10235.0
24	12503.5
25	14772.0
26	14772.0
27	16981.0
28	19190.0
29	22412.0
30	25634.0
31	30843.5
32	36053.0
33	36053.0
34	41915.0
35	47777.0
36	54569.5
37	61362.0
38	66307.0
39	71252.0
40	71252.0
41	76662.5
42	82073.0
43	87423.0
44	92773.0
45	97610.0
46	102447.0
47	102447.0
48	106270.5
49	110094.0
50	115112.0
51	120130.0
52	125844.5
53	131559.0
54	131559.0
55	128776.0
56	125993.0
57	123651.0
58	121309.0
59	114522.5
60	107736.0
61	107736.0
62	101216.0
63	94696.0
64	85363.5
65	76031.0
66	67281.5
67	58532.0
68	58532.0
69	50556.0
70	42580.0
71	36862.5
72	31145.0
73	25809.0
74	20473.0
75	20473.0
76	16452.5
77	12432.0
78	10351.5
79	8271.0
80	6526.5
81	4782.0
82	4782.0
83	3663.5
84	2545.0
85	1988.5
86	1432.0
87	1081.5
88	731.0
89	731.0
90	549.0
91	367.0
92	226.5
93	86.0
94	52.5
95	19.0
96	19.0
97	15.5
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1661776.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.02619402607621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.07805604194249	57.69484485654698
2	8.157523796886814	10.93535545564939
3	2.347576433780132	4.720473406247735
4	1.0186833137481872	2.7311386175364905
5	0.5701606639398712	1.9107849643635118
6	0.3665676188950932	1.4741779408643565
7	0.25749459569409155	1.2081217912160758
8	0.18198022415039894	0.9757953450250783
9	0.13677854229246486	0.8250970602868758
>10	0.7797712098993977	9.645055223376794
>50	0.06929288448439284	3.2255240783598427
>100	0.03548273568309201	4.116612117465716
>500	3.611077734452217E-4	0.15429845800630673
>1k	2.7083083008391627E-4	0.38272068505485884
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2497	0.15026092566025745	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2300	0.13840613897420592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	6.0176582162698224E-5	0.0	0.0	0.0
5	0.0	1.2035316432539645E-4	0.0	0.0	0.0
6	0.0	1.2035316432539645E-4	0.0	0.0	0.0
7	0.0	1.2035316432539645E-4	0.0	0.0	0.0
8	0.0	1.2035316432539645E-4	0.0	0.0	0.0
9	0.0	1.2035316432539645E-4	0.0	0.0	0.0
10	0.0	1.2035316432539645E-4	0.0	0.0	0.0
11	0.0	1.2035316432539645E-4	0.0	0.0	0.0
12	0.0	1.2035316432539645E-4	0.0	6.0176582162698224E-5	0.0
13	0.0	1.2035316432539645E-4	0.0	6.0176582162698224E-5	0.0
14	0.0	1.2035316432539645E-4	0.0	6.0176582162698224E-5	0.0
15	0.0	1.2035316432539645E-4	0.0	2.407063286507929E-4	0.0
16	0.0	1.2035316432539645E-4	0.0	6.017658216269823E-4	6.0176582162698224E-5
17	0.0	1.2035316432539645E-4	0.0	0.0011433550610912661	6.0176582162698224E-5
18	0.0	1.2035316432539645E-4	0.0	0.0012637082254166627	6.0176582162698224E-5
19	0.0	1.2035316432539645E-4	0.0	0.0015044145540674555	6.0176582162698224E-5
20	0.0	1.2035316432539645E-4	0.0	0.0017451208827182485	6.0176582162698224E-5
21	0.0	1.2035316432539645E-4	0.0	0.0020460037935317397	6.0176582162698224E-5
22	0.0	1.2035316432539645E-4	0.0	0.003189358854623006	6.0176582162698224E-5
23	0.0	1.2035316432539645E-4	0.0	0.004633596826527763	6.0176582162698224E-5
24	0.0	1.2035316432539645E-4	0.0	0.006860130366547597	6.0176582162698224E-5
25	0.0	1.2035316432539645E-4	0.0	0.008785780995753941	6.0176582162698224E-5
26	0.0	1.2035316432539645E-4	0.0	0.01384061389742059	1.2035316432539645E-4
27	0.0	1.2035316432539645E-4	0.0	0.037369657523035595	1.2035316432539645E-4
28	0.0	1.2035316432539645E-4	0.0	0.09664359095329335	1.2035316432539645E-4
29	0.0	1.2035316432539645E-4	0.0	0.17372979270370978	1.2035316432539645E-4
30	0.0	1.2035316432539645E-4	0.0	0.2800016368030348	1.2035316432539645E-4
31	0.0	1.2035316432539645E-4	0.0	0.5468246021124388	1.2035316432539645E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	45	1.3235591E-4	24.666666	1
GGTATCA	1360	0.0	19.860294	1
TATACGG	65	0.0015803349	17.076923	2
CTTATAC	1120	0.0	16.1875	37
TCTTATA	1935	0.0	15.58398	37
AATACTG	280	0.0	14.535714	5
GGTTAAG	130	4.446545E-6	14.230769	1
GTATCAA	1930	0.0	13.994818	2
CGCTAAC	80	0.00630018	13.875	11
TCGTTCG	95	0.001245482	13.631579	9
TACGCTA	95	0.001245482	13.631579	9
GTTCGCG	95	0.001245482	13.631579	30
TATACGC	95	0.001245482	13.631579	7
TATCGGA	95	0.001245482	13.631579	17
GTTATCG	95	0.001245482	13.631579	15
TAAGACT	245	7.2759576E-12	13.591836	4
CGAATTA	150	1.3063836E-6	13.566667	15
CTCTTAT	3030	0.0	13.554454	37
GTATAGA	180	5.16211E-8	13.361111	1
TTATGAC	130	7.001482E-5	12.807693	3
>>END_MODULE