FastQCFastQC Report
Fri 10 Feb 2017
ERR1632119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254336
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCAATA250.005488396629.634
CTAATAC652.669227E-622.769233
GGTATCA1005.293259E-1022.21
CCTACTC450.003817742520.5555553
TTATAGA450.003817742520.5555554
CTTGCAC450.003817742520.5555553
GTATAGA450.003817742520.5555551
TTTGACA450.003817742520.5555554
TAAGCAG450.003817742520.5555555
CGAAGAG450.003817742520.55555518
CTATACT500.007020192718.54
TAAAACT500.007020192718.54
CTATTAT500.007020192718.51
TATACAG500.007020192718.55
CGTCCAC500.007020192718.510
GATTAAG500.007020192718.51
TTTTAAG609.209881E-418.52
GGGGCGC609.209881E-418.516
TCTTATA3850.018.25973937
CTTATAC1850.018.037