##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632119.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	254336
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.175614148213388	31.0	31.0	34.0	30.0	34.0
2	31.345126132360342	31.0	31.0	34.0	30.0	34.0
3	31.38611521766482	31.0	31.0	34.0	28.0	34.0
4	35.27846234901862	37.0	35.0	37.0	33.0	37.0
5	34.32730718419728	35.0	35.0	37.0	30.0	37.0
6	34.26512172873679	35.0	35.0	37.0	30.0	37.0
7	34.257328887770505	35.0	35.0	37.0	30.0	37.0
8	34.188097634625066	35.0	35.0	37.0	30.0	37.0
9	35.75802481756417	37.0	35.0	39.0	30.0	39.0
10	35.37633288248616	37.0	34.0	39.0	30.0	39.0
11	35.61888604051334	37.0	35.0	39.0	30.0	39.0
12	35.4815676899849	37.0	35.0	39.0	30.0	39.0
13	35.62561336185204	37.0	35.0	39.0	30.0	39.0
14	36.55023276295923	38.0	36.0	40.0	31.0	41.0
15	36.51278623553095	38.0	36.0	40.0	31.0	41.0
16	36.3896381165073	38.0	35.0	40.0	31.0	41.0
17	36.37685581278309	38.0	35.0	40.0	31.0	41.0
18	36.380186053095116	38.0	35.0	40.0	31.0	41.0
19	36.44315000629089	38.0	35.0	40.0	30.0	41.0
20	36.36894108580775	38.0	35.0	40.0	30.0	41.0
21	36.326591595369905	38.0	35.0	40.0	30.0	41.0
22	36.2795907775541	38.0	35.0	40.0	30.0	41.0
23	36.211413248616005	38.0	35.0	40.0	30.0	41.0
24	36.09573949421238	38.0	34.0	40.0	30.0	41.0
25	35.97033451811777	38.0	34.0	40.0	29.0	41.0
26	35.791688946904884	38.0	34.0	40.0	29.0	41.0
27	35.67142284222446	38.0	34.0	40.0	28.0	41.0
28	35.515432341469555	38.0	34.0	40.0	27.0	41.0
29	35.3226086751384	38.0	34.0	40.0	27.0	41.0
30	35.20858234776044	38.0	34.0	40.0	27.0	41.0
31	35.09004230624056	38.0	33.0	40.0	27.0	40.0
32	34.947439607448416	38.0	33.0	40.0	26.0	40.0
33	34.74814025541016	37.0	33.0	39.0	26.0	40.0
34	34.71937122546552	37.0	33.0	39.0	26.0	40.0
35	34.65674933945647	37.0	33.0	39.0	25.0	40.0
36	34.63789632611978	37.0	33.0	39.0	25.0	40.0
37	34.57924163311525	37.0	33.0	39.0	25.0	40.0
38	34.503727352793156	37.0	33.0	39.0	25.0	40.0
39	34.31249213638651	37.0	33.0	39.0	25.0	40.0
40	34.26771672118772	37.0	32.0	39.0	25.0	40.0
41	34.251934448917964	37.0	32.0	39.0	25.0	40.0
42	34.102278088827376	37.0	32.0	39.0	24.0	40.0
43	33.06026280196276	36.0	30.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	2.0
15	7.0
16	18.0
17	29.0
18	54.0
19	109.0
20	169.0
21	289.0
22	519.0
23	746.0
24	1124.0
25	1751.0
26	2560.0
27	3606.0
28	5040.0
29	6621.0
30	8744.0
31	11440.0
32	14986.0
33	19352.0
34	24740.0
35	31366.0
36	37302.0
37	39876.0
38	33562.0
39	10322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57478296426774	18.01711122294917	12.070646703573226	27.337459109209867
2	20.062437091092097	20.21577755410166	32.04776358832411	27.67402176648213
3	20.781564544539506	21.27972445898339	28.644391670860593	29.29431932561651
4	16.011889783593354	15.387125691997989	33.05705837946653	35.54392614494212
5	16.799823855057877	34.21418910417715	32.91197471061903	16.074012330145948
6	37.0926648213387	33.639358958228485	14.550830397584297	14.717145822848515
7	31.8574641419225	28.768636763965777	19.637408782083543	19.73649031202818
8	29.601786612984398	31.897175390035233	18.628900352289886	19.872137644690486
9	27.848593985908405	13.212836562657273	17.506762707599396	41.431806743834926
10	17.711609838953198	25.663295797684953	31.518542400603927	25.106551962757926
11	39.49460556114746	19.682624559637645	19.816699169602416	21.00607070961248
12	24.002107448414694	23.22832788122798	27.3818885254152	25.387676144942123
13	32.85496351283342	17.62432372924006	22.188364997483646	27.332347760442875
14	24.348892803220938	18.19050390035229	22.819813160543532	34.64079013588324
15	27.047291771514846	25.307074106693506	20.09546426774031	27.550169854051333
16	27.33392048314041	23.960037116255663	21.758618520382488	26.94742388022144
17	26.242450931051835	24.13421615500755	23.202771137393054	26.420561776547558
18	26.648606567689985	23.225968797181682	23.518888399597383	26.606536235530953
19	27.377170357322594	23.25624370910921	23.17957347760443	26.187012455963764
20	27.40901799194766	23.041567060895822	22.965290010065427	26.58412493709109
21	27.843875817815807	23.068696527428283	22.475780070457976	26.611647584297938
22	28.093152365374934	23.15283719174635	22.41405070457977	26.339959738298944
23	27.397615752390536	22.81391545042778	22.671584046300953	27.116884750880725
24	27.048078132863612	23.038421615500752	22.501729994967288	27.411770256668344
25	27.313868268746855	22.34327818319074	23.019155762455963	27.323697785606445
26	26.841265098137896	22.82610405133367	23.059653371917463	27.272977478610972
27	26.67337695017615	23.05611474584801	22.785213261197786	27.485295042778056
28	26.686745093105184	23.16384625062909	23.117057750377455	27.032350905888276
29	26.556209109209867	23.170530322093608	23.17524849018621	27.09801207851032
30	26.51767740312028	23.430422747861098	22.938946904881732	27.112952944136893
31	27.000110090588826	23.20670294413689	22.544979869149472	27.24820709612481
32	26.263289506794163	23.183898465022647	23.07616696024157	27.476645067941618
33	26.12882171615501	22.96332410669351	23.785464896829392	27.122389280322096
34	26.725276799194763	22.351534977352795	23.706042400603923	27.217145822848515
35	26.47246162556618	22.79582913940614	24.00957788122798	26.7221313537997
36	26.333668847508807	22.561886638147964	23.86763965777554	27.23680485656769
37	25.934983643683946	21.827425138399597	24.56396263210871	27.67362858580775
38	25.432105561147463	22.055469929542024	25.097508807247102	27.414915702063414
39	25.27129466532461	21.567139531957725	25.693963890286863	27.4676019124308
40	24.899345747357827	21.195976975339708	26.90024219929542	27.004435078007045
41	24.423597131353798	20.946700427780574	27.362622672370406	27.26707976849522
42	24.076025415198792	20.970684448917968	27.785291897332666	27.167998238550577
43	23.18861663311525	20.324688600905887	28.543737418218416	27.942957347760444
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.5
2	3.0
3	10.0
4	17.0
5	17.0
6	24.0
7	31.0
8	27.0
9	23.0
10	33.5
11	44.0
12	44.0
13	78.5
14	113.0
15	217.5
16	322.0
17	348.0
18	374.0
19	374.0
20	408.5
21	443.0
22	526.0
23	609.0
24	808.5
25	1008.0
26	1008.0
27	1307.0
28	1606.0
29	2047.5
30	2489.0
31	3022.5
32	3556.0
33	3556.0
34	4500.5
35	5445.0
36	6454.5
37	7464.0
38	8545.0
39	9626.0
40	9626.0
41	10714.0
42	11802.0
43	13026.5
44	14251.0
45	15093.5
46	15936.0
47	15936.0
48	17169.5
49	18403.0
50	19057.5
51	19712.0
52	20297.5
53	20883.0
54	20883.0
55	20836.0
56	20789.0
57	20496.0
58	20203.0
59	19750.5
60	19298.0
61	19298.0
62	17986.0
63	16674.0
64	14847.0
65	13020.0
66	11596.0
67	10172.0
68	10172.0
69	8642.5
70	7113.0
71	6081.0
72	5049.0
73	4109.0
74	3169.0
75	3169.0
76	2570.5
77	1972.0
78	1581.0
79	1190.0
80	959.0
81	728.0
82	728.0
83	575.0
84	422.0
85	317.5
86	213.0
87	149.5
88	86.0
89	86.0
90	69.0
91	52.0
92	36.0
93	20.0
94	11.0
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	254336.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.14077769707613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.2403451470988	77.61174375723614
2	4.781790435972239	8.046871321342898
3	1.2567070657834636	3.1722092955729373
4	0.5629783145970454	1.8947773286713834
5	0.3331752659779266	1.401681299440647
6	0.20139172018076046	1.0167153574656675
7	0.13388692104001731	0.7885744761841857
8	0.09127515673929838	0.6143970137973669
9	0.06923422062224494	0.5242879049765972
>10	0.3203131654363836	4.385863216049237
>50	0.007965472178001084	0.43446092247478785
>100	9.371143738824738E-4	0.10841810678817237
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.931806743834927E-4	0.0	3.931806743834927E-4	0.0
3	0.0	3.931806743834927E-4	0.0	3.931806743834927E-4	0.0
4	0.0	3.931806743834927E-4	0.0	3.931806743834927E-4	0.0
5	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
6	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
7	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
8	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
9	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
10	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
11	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
12	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
13	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
14	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
15	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
16	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
17	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
18	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
19	0.0	7.863613487669854E-4	0.0	3.931806743834927E-4	0.0
20	0.0	7.863613487669854E-4	0.0	7.863613487669854E-4	0.0
21	0.0	7.863613487669854E-4	0.0	0.0015727226975339708	0.0
22	0.0	7.863613487669854E-4	0.0	0.002752264720684449	0.0
23	0.0	7.863613487669854E-4	0.0	0.002752264720684449	0.0
24	0.0	7.863613487669854E-4	0.0	0.003538626069451434	0.0
25	0.0	7.863613487669854E-4	0.0	0.003931806743834927	0.0
26	0.0	7.863613487669854E-4	0.0	0.005897710115752391	0.0
27	0.0	7.863613487669854E-4	0.0	0.018479491696024156	0.0
28	0.0	7.863613487669854E-4	0.0	0.080602038248616	0.0
29	0.0	7.863613487669854E-4	0.0	0.18715400100654253	0.0
30	0.0	7.863613487669854E-4	0.0	0.3275195017614494	0.0
31	0.0	7.863613487669854E-4	0.0	0.7073320332159033	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCAATA	25	0.0054883966	29.6	34
CTAATAC	65	2.669227E-6	22.76923	3
GGTATCA	100	5.293259E-10	22.2	1
CCTACTC	45	0.0038177425	20.555555	3
TTATAGA	45	0.0038177425	20.555555	4
CTTGCAC	45	0.0038177425	20.555555	3
GTATAGA	45	0.0038177425	20.555555	1
TTTGACA	45	0.0038177425	20.555555	4
TAAGCAG	45	0.0038177425	20.555555	5
CGAAGAG	45	0.0038177425	20.555555	18
CTATACT	50	0.0070201927	18.5	4
TAAAACT	50	0.0070201927	18.5	4
CTATTAT	50	0.0070201927	18.5	1
TATACAG	50	0.0070201927	18.5	5
CGTCCAC	50	0.0070201927	18.5	10
GATTAAG	50	0.0070201927	18.5	1
TTTTAAG	60	9.209881E-4	18.5	2
GGGGCGC	60	9.209881E-4	18.5	16
TCTTATA	385	0.0	18.259739	37
CTTATAC	185	0.0	18.0	37
>>END_MODULE