FastQCFastQC Report
Fri 10 Feb 2017
ERR1632118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105194
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37380.33822116298134086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT24960.22584270272911364No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23120.20919404195100585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAC250.005496253729.5999983
GGTATCA15350.020.48861
CGAAATA609.2365104E-418.524
ATACCGT902.1520627E-618.56
TATACAC4200.017.6190537
TAGACCA953.606132E-617.5263164
TTAGGGT752.0671762E-417.2666664
ACTCTAT650.001579882317.0769236
GCGCATT650.001579882317.07692311
GACGGAC1655.4569682E-1116.8181827
ACGGACC1554.0017767E-1016.7096778
CTATATC803.3824178E-416.18752
ATGTGCG803.3824178E-416.18757
GAATATG1151.2420132E-616.0869561
TCTTATA17050.015.95014737
GCATTAC1403.472087E-815.8571431
TAGGGAC700.002592408615.8571434
TCGTTTA1302.5884947E-715.65384630
GTTCAAA2600.015.6538461
CGGACCA1902.7284841E-1115.5789489
Produced by FastQC (version 0.11.5)