##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632118.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1105194
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.268159255298166	33.0	31.0	34.0	30.0	34.0
2	32.43055698818488	34.0	31.0	34.0	31.0	34.0
3	32.44783359301625	34.0	31.0	34.0	31.0	34.0
4	36.061065297133354	37.0	35.0	37.0	35.0	37.0
5	35.86815255964111	37.0	35.0	37.0	35.0	37.0
6	35.883846636880044	37.0	35.0	37.0	35.0	37.0
7	35.86792002128133	37.0	35.0	37.0	35.0	37.0
8	35.84035653468984	37.0	35.0	37.0	35.0	37.0
9	37.55690765603143	39.0	37.0	39.0	35.0	39.0
10	37.41590888115571	39.0	37.0	39.0	35.0	39.0
11	37.55380865259855	39.0	37.0	39.0	35.0	39.0
12	37.49009133238147	39.0	37.0	39.0	35.0	39.0
13	37.54392170062451	39.0	37.0	39.0	35.0	39.0
14	38.780875574785966	40.0	38.0	41.0	36.0	41.0
15	38.77828417454311	40.0	38.0	41.0	35.0	41.0
16	38.65968689659915	40.0	38.0	41.0	35.0	41.0
17	38.707296637513416	40.0	38.0	41.0	35.0	41.0
18	38.734762403704686	40.0	38.0	41.0	35.0	41.0
19	38.78348778585479	40.0	38.0	41.0	35.0	41.0
20	38.757076133239956	40.0	38.0	41.0	35.0	41.0
21	38.72312191343782	40.0	38.0	41.0	35.0	41.0
22	38.694810141929835	40.0	38.0	41.0	35.0	41.0
23	38.64091100747923	40.0	38.0	41.0	35.0	41.0
24	38.57325139296811	40.0	38.0	41.0	34.0	41.0
25	38.48774423313916	40.0	38.0	41.0	34.0	41.0
26	38.37405559566918	40.0	38.0	41.0	34.0	41.0
27	38.28719211287792	40.0	38.0	41.0	34.0	41.0
28	38.19954777170343	40.0	38.0	41.0	34.0	41.0
29	38.03433786285485	40.0	38.0	41.0	33.0	41.0
30	37.96709627450022	40.0	37.0	41.0	33.0	41.0
31	37.85908989733929	40.0	37.0	41.0	33.0	41.0
32	37.75553070320686	40.0	37.0	41.0	33.0	41.0
33	37.590991264882	40.0	37.0	41.0	33.0	41.0
34	37.57549896217316	40.0	37.0	41.0	33.0	41.0
35	37.532128296027665	39.0	37.0	41.0	33.0	41.0
36	37.50212722834181	39.0	37.0	41.0	33.0	41.0
37	37.457978418268645	39.0	36.0	41.0	33.0	41.0
38	37.380512380631814	39.0	36.0	41.0	32.0	41.0
39	37.179121493602025	39.0	36.0	41.0	32.0	41.0
40	37.16711636147138	39.0	36.0	41.0	31.0	41.0
41	37.14704024813743	39.0	36.0	41.0	32.0	41.0
42	37.00393053165327	39.0	36.0	40.0	31.0	41.0
43	36.07349207469458	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	1.0
14	2.0
15	9.0
16	8.0
17	18.0
18	38.0
19	95.0
20	151.0
21	317.0
22	530.0
23	899.0
24	1328.0
25	2017.0
26	3082.0
27	4732.0
28	6666.0
29	9243.0
30	12776.0
31	16755.0
32	22720.0
33	30684.0
34	43822.0
35	63026.0
36	98809.0
37	173537.0
38	332938.0
39	280985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48114448685027	17.41685170205412	12.309422599109297	26.79258121198631
2	20.047249623143088	19.993865330430676	32.379292685266115	27.57959236116012
3	20.391985479472382	20.95740657296366	29.62312498982079	29.027482957743167
4	15.44597600059356	15.22881955566172	33.07292656311924	36.252277880625485
5	16.541982674534967	34.283121334353964	32.795237759162646	16.37965823194842
6	36.1709346956281	33.61418900211184	14.862458536691294	15.35241776556876
7	30.95791327133517	29.366608939245054	19.758612515087847	19.916865274331926
8	29.588198994927588	31.009397445154423	18.947714156971536	20.454689402946453
9	27.783357491987832	13.130907333916037	18.0932940280168	40.99244114607933
10	18.082436205770207	25.62373664714068	31.157968646228625	25.13585850086048
11	38.72424207876627	19.917860574704534	19.712104843131613	21.645792503397594
12	23.471263868605874	24.381058891018228	27.378632167746115	24.769045072629783
13	33.1492932462536	17.823567627041044	22.42429835847824	26.602840768227114
14	24.19620446727	18.98797858113598	23.59296195961976	33.22285499197426
15	27.222098563691084	25.55379417550222	20.743055065445525	26.481052195361176
16	26.90794557335635	24.187699173176835	22.297623765601333	26.60673148786548
17	25.30134980826896	24.55514597437192	23.827490920146147	26.316013297212976
18	25.795018793080672	22.959769958939336	24.944941793024572	26.300269454955423
19	26.38867022441309	23.46773507637573	24.593057870382935	25.550536828828243
20	26.857637663613808	23.026636047607933	24.225430105483742	25.890296183294513
21	27.844613705829026	23.289485827827512	23.34685132203034	25.519049144313126
22	27.70337153477127	23.166882918293076	23.316087492331665	25.813658054603987
23	26.94585746936737	23.09775478332311	23.469635195268886	26.486752552040638
24	26.363425787689764	23.308034607498772	23.695025488737723	26.633514116073737
25	26.25882876671426	22.932082512210524	24.0155122087163	26.793576512358918
26	26.28172067528416	23.13159499599165	23.87010787246402	26.716576456260167
27	26.03497666473035	23.33165037088511	23.93471191483124	26.69866104955329
28	25.990912002779602	23.307944125646717	24.417613559248423	26.283530312325258
29	25.646990483118802	23.738185332167927	24.504747582777323	26.11007660193595
30	25.651876503129767	24.03976134506702	24.209324335817964	26.09903781598525
31	26.27439164526771	23.852916320573584	23.567355595488213	26.305336438670494
32	25.214306266592107	23.790755288211844	24.386487802141524	26.60845064305452
33	25.08057408925492	23.465201584518194	25.047367249550756	26.406857076676133
34	25.76253580819295	23.072781792155947	24.932364815588937	26.232317584062166
35	25.351205308751222	23.196560965767095	25.488285314614444	25.963948410867232
36	25.236655284049682	23.096669001098448	24.96819562900269	26.69848008584918
37	24.81301925272848	22.360418170927456	25.7616309896724	27.06493158667166
38	24.325955443116772	22.311015079705463	26.590082827087368	26.77294665009039
39	24.368210468026426	21.72252111394018	27.18762497805815	26.721643439975246
40	23.942221908551804	21.512874662728894	28.267887809742	26.277015618977302
41	23.309029907871377	21.206593593522946	28.895379453742965	26.58899704486271
42	22.903309283257055	21.22224695392845	29.262011918269554	26.612431844544936
43	21.98871872268579	20.682341742716666	29.97654710394736	27.352392430650184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	19.5
2	28.0
3	131.0
4	234.0
5	234.0
6	336.5
7	439.0
8	449.5
9	460.0
10	741.0
11	1022.0
12	1022.0
13	1898.0
14	2774.0
15	4796.0
16	6818.0
17	6173.0
18	5528.0
19	5528.0
20	6002.5
21	6477.0
22	5703.5
23	4930.0
24	5892.5
25	6855.0
26	6855.0
27	8080.5
28	9306.0
29	11171.5
30	13037.0
31	15394.5
32	17752.0
33	17752.0
34	21206.0
35	24660.0
36	28857.5
37	33055.0
38	37018.5
39	40982.0
40	40982.0
41	45891.5
42	50801.0
43	55173.5
44	59546.0
45	64264.0
46	68982.0
47	68982.0
48	74476.0
49	79970.0
50	82496.5
51	85023.0
52	86886.5
53	88750.0
54	88750.0
55	88539.5
56	88329.0
57	88373.5
58	88418.0
59	84511.5
60	80605.0
61	80605.0
62	74272.0
63	67939.0
64	60682.0
65	53425.0
66	45708.0
67	37991.0
68	37991.0
69	32899.5
70	27808.0
71	24234.0
72	20660.0
73	16545.0
74	12430.0
75	12430.0
76	10121.0
77	7812.0
78	6412.0
79	5012.0
80	4132.0
81	3252.0
82	3252.0
83	2604.5
84	1957.0
85	1516.5
86	1076.0
87	851.0
88	626.0
89	626.0
90	466.5
91	307.0
92	196.0
93	85.0
94	50.5
95	16.0
96	16.0
97	10.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1105194.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.24136519627393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.7835157833555	55.966336810422234
2	7.922554634216265	10.337565603566715
3	2.308698981259028	4.518680200937574
4	1.085064024439073	2.83164233119073
5	0.6582702985458447	2.1473226472644864
6	0.4366981550753424	1.7094470289485681
7	0.30357464633779657	1.3863937056237723
8	0.2241562236231102	1.169940643713038
9	0.1779023077170022	1.0445930484322357
>10	1.003227667361863	12.212235768098656
>50	0.0712944036515005	3.1651946388454957
>100	0.024486366759830635	2.646442604256653
>500	1.3912691447893433E-4	0.08849909090247152
>1k	4.17380743436803E-4	0.775705877797458
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3738	0.33822116298134086	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2496	0.22584270272911364	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2312	0.20919404195100585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.048185205493334E-5	0.0
3	0.0	0.0	0.0	9.048185205493334E-5	0.0
4	0.0	0.0	0.0	9.048185205493334E-5	0.0
5	0.0	1.8096370410986668E-4	0.0	1.8096370410986668E-4	0.0
6	0.0	1.8096370410986668E-4	0.0	1.8096370410986668E-4	0.0
7	0.0	1.8096370410986668E-4	0.0	1.8096370410986668E-4	0.0
8	0.0	1.8096370410986668E-4	0.0	1.8096370410986668E-4	0.0
9	0.0	1.8096370410986668E-4	0.0	3.6192740821973336E-4	0.0
10	0.0	1.8096370410986668E-4	0.0	3.6192740821973336E-4	0.0
11	0.0	1.8096370410986668E-4	0.0	3.6192740821973336E-4	0.0
12	0.0	1.8096370410986668E-4	0.0	4.524092602746667E-4	0.0
13	0.0	1.8096370410986668E-4	0.0	5.428911123296E-4	0.0
14	0.0	1.8096370410986668E-4	0.0	5.428911123296E-4	0.0
15	0.0	1.8096370410986668E-4	0.0	6.333729643845334E-4	0.0
16	0.0	1.8096370410986668E-4	0.0	9.953003726042668E-4	0.0
17	0.0	1.8096370410986668E-4	0.0	0.0012667459287690668	0.0
18	0.0	1.8096370410986668E-4	0.0	0.0012667459287690668	0.0
19	0.0	1.8096370410986668E-4	0.0	0.0014477096328789334	0.0
20	0.0	1.8096370410986668E-4	0.0	0.0017191551890437336	0.0
21	0.0	1.8096370410986668E-4	0.0	0.0021715644493184	0.0
22	0.0	1.8096370410986668E-4	0.0	0.002623973709593067	0.0
23	0.0	2.714455561648E-4	0.0	0.0038002377863072005	0.0
24	0.0	2.714455561648E-4	0.0	0.006605175200010134	0.0
25	0.0	2.714455561648E-4	0.0	0.0086862577972736	0.0
26	0.0	2.714455561648E-4	0.0	0.011762640767141334	0.0
27	0.0	2.714455561648E-4	0.0	0.0331163578521056	0.0
28	0.0	3.6192740821973336E-4	0.0	0.10785436764948055	0.0
29	0.0	3.6192740821973336E-4	0.0	0.22756185791815736	0.0
30	0.0	3.6192740821973336E-4	0.0	0.3962200301485531	0.0
31	0.0	3.6192740821973336E-4	0.0	0.8276375007464752	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAAC	25	0.0054962537	29.599998	3
GGTATCA	1535	0.0	20.4886	1
CGAAATA	60	9.2365104E-4	18.5	24
ATACCGT	90	2.1520627E-6	18.5	6
TATACAC	420	0.0	17.61905	37
TAGACCA	95	3.606132E-6	17.526316	4
TTAGGGT	75	2.0671762E-4	17.266666	4
ACTCTAT	65	0.0015798823	17.076923	6
GCGCATT	65	0.0015798823	17.076923	11
GACGGAC	165	5.4569682E-11	16.818182	7
ACGGACC	155	4.0017767E-10	16.709677	8
CTATATC	80	3.3824178E-4	16.1875	2
ATGTGCG	80	3.3824178E-4	16.1875	7
GAATATG	115	1.2420132E-6	16.086956	1
TCTTATA	1705	0.0	15.950147	37
GCATTAC	140	3.472087E-8	15.857143	1
TAGGGAC	70	0.0025924086	15.857143	4
TCGTTTA	130	2.5884947E-7	15.653846	30
GTTCAAA	260	0.0	15.653846	1
CGGACCA	190	2.7284841E-11	15.578948	9
>>END_MODULE