FastQCFastQC Report
Fri 10 Feb 2017
ERR1632117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5737
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT1432.4925919470106326RNA PCR Primer, Index 39 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT691.2027191912149207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT621.0807042008018126No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT470.8192435070594387No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC460.8018127941432804RNA PCR Primer, Index 39 (95% over 21bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC360.6275056649816977RNA PCR Primer, Index 39 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC280.4880599616524316No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG230.4009063970716402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT220.38347568415548194No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.31375283249084884No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG140.2440299808262158No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.20916855499389925No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA120.20916855499389925No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG80.13944570332926615No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT80.13944570332926615No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1220149904131079No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAA70.1220149904131079No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT70.1220149904131079No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC60.10458427749694962No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA60.10458427749694962No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC60.10458427749694962No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC60.10458427749694962No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTA60.10458427749694962No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT60.10458427749694962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA400.001706705923.1251
CCGTCTT450.003385036720.55555537
CTCCGAG450.003385036720.5555552
AGCCCAC500.006231613518.57
ATCTCGT500.006231613518.527
GCCCACG500.006231613518.58
CCACGAG500.006231613518.510
GAGCCCA500.006231613518.56
CCCACGA500.006231613518.59
TCTACAT500.006231613518.522
CTCTACA500.006231613518.521
TACATCT500.006231613518.524
ACGAGAC500.006231613518.512
ACCTCTC500.006231613518.517
CGAGCCC500.006231613518.55
CCGAGCC500.006231613518.54
AGACCTC500.006231613518.515
TCTCTAC500.006231613518.520
GCCGTCT500.006231613518.536
CGAGACC500.006231613518.513
Produced by FastQC (version 0.11.5)