##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632117.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5737
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93533205508105	33.0	31.0	34.0	30.0	34.0
2	32.059787345302425	33.0	31.0	34.0	30.0	34.0
3	32.04253093951542	34.0	31.0	34.0	30.0	34.0
4	35.78595084538958	37.0	35.0	37.0	35.0	37.0
5	35.49119748997734	37.0	35.0	37.0	33.0	37.0
6	35.557957120446225	37.0	35.0	37.0	33.0	37.0
7	35.436813665678926	37.0	35.0	37.0	33.0	37.0
8	35.55150775666725	37.0	35.0	37.0	33.0	37.0
9	37.187554470977865	39.0	37.0	39.0	34.0	39.0
10	37.08140142931846	39.0	37.0	39.0	33.0	39.0
11	37.14850967404567	39.0	37.0	39.0	33.0	39.0
12	37.06484225204811	39.0	37.0	39.0	33.0	39.0
13	37.115042705246644	39.0	37.0	39.0	33.0	39.0
14	38.160188251699495	40.0	38.0	41.0	34.0	41.0
15	38.18703154959038	40.0	38.0	41.0	34.0	41.0
16	37.96775318110511	40.0	37.0	41.0	33.0	41.0
17	38.09063970716402	40.0	37.0	41.0	34.0	41.0
18	38.252745337284296	40.0	38.0	41.0	34.0	41.0
19	38.302422869095345	40.0	38.0	41.0	34.0	41.0
20	38.273836499912846	40.0	38.0	41.0	34.0	41.0
21	38.305386090291094	40.0	38.0	41.0	34.0	41.0
22	38.217535297193656	40.0	38.0	41.0	34.0	41.0
23	38.17395851490326	40.0	38.0	41.0	34.0	41.0
24	37.99755970019174	40.0	37.0	41.0	33.0	41.0
25	37.875370402649466	40.0	37.0	41.0	33.0	41.0
26	37.75004357678229	40.0	37.0	41.0	33.0	41.0
27	37.55813142757539	39.0	37.0	41.0	32.0	41.0
28	37.51106850270176	39.0	37.0	40.0	32.0	41.0
29	37.35506362210214	39.0	36.0	40.0	32.0	41.0
30	37.25030503747603	39.0	36.0	40.0	32.0	41.0
31	36.99668816454593	39.0	35.0	40.0	31.0	41.0
32	36.83841729126721	39.0	35.0	40.0	31.0	41.0
33	36.58410318982046	39.0	35.0	40.0	30.0	41.0
34	36.50880251002266	38.0	35.0	40.0	30.0	41.0
35	36.46452849921562	38.0	35.0	40.0	30.0	41.0
36	36.370925570855846	38.0	35.0	40.0	30.0	41.0
37	36.2776712567544	38.0	35.0	40.0	30.0	41.0
38	36.22485619661844	38.0	35.0	40.0	30.0	41.0
39	35.98553250827959	38.0	35.0	40.0	29.0	41.0
40	35.66881645459299	38.0	35.0	40.0	28.0	41.0
41	35.650165591772705	38.0	35.0	40.0	27.0	41.0
42	35.480041833711	38.0	35.0	40.0	27.0	41.0
43	34.47707861251525	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	1.0
18	0.0
19	0.0
20	1.0
21	2.0
22	8.0
23	10.0
24	14.0
25	20.0
26	32.0
27	43.0
28	53.0
29	77.0
30	100.0
31	138.0
32	191.0
33	222.0
34	287.0
35	407.0
36	609.0
37	1080.0
38	1541.0
39	900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.759456161757015	18.86003137528325	18.02335715530765	23.357155307652082
2	18.110510719888442	22.241589681017953	31.793620359072687	27.85427924002092
3	20.3416419731567	21.77096043228168	34.0421823252571	23.845215269304514
4	14.223461739585149	17.83161931322991	29.8239497995468	38.12096914763814
5	14.72895241415374	34.96601010981349	32.246818894892805	18.05821858113997
6	32.97890883737145	32.75230956946139	15.530765208297021	18.73801638487014
7	24.78647376677706	33.344953808610775	19.13892278194178	22.729649642670385
8	32.49084887571902	30.102841206205333	18.284817849050025	19.12149206902562
9	24.263552379292314	13.61338678751961	18.354540700714658	43.76852013247342
10	20.010458427749693	26.494683632560573	26.686421474638312	26.80843646505142
11	34.37336587066411	21.596653303120096	17.535297193655218	26.494683632560573
12	20.481087676485966	30.678054732438557	24.542443785950844	24.298413805124632
13	34.39079658358027	16.5417465574342	23.49660101098135	25.570855848004182
14	23.862645982220673	21.823252571030157	24.59473592469932	29.719365522049852
15	29.33588983789437	26.63412933588984	19.400383475684155	24.629597350531636
16	27.784556388356286	23.880076695136832	24.19382952762768	24.141537388879204
17	21.004009063970717	31.56702109116263	23.723200278891408	23.70576956597525
18	21.997559700191736	21.718668293533206	27.383649991284642	28.900122014990416
19	23.27000174307129	26.63412933588984	26.442391493812096	23.653477427226775
20	24.07181453721457	21.526930451455463	30.538609029109292	23.862645982220673
21	27.523095694613907	20.7599790831445	25.30939515426181	26.407530067979778
22	26.16350008715356	23.3920167334844	27.139620010458426	23.30486316890361
23	23.531462436813666	23.49660101098135	24.873627331357852	28.098309220847135
24	22.95624891058044	24.681889489280113	29.12672128290047	23.235140317238976
25	22.328743245598744	26.268084364650512	25.483702283423394	25.919470106327346
26	23.914938120969147	23.601185288478298	25.989192957991982	26.494683632560573
27	21.87554470977863	27.10475858462611	26.442391493812096	24.577305211783163
28	21.85811399686247	22.415896810179536	29.858811225379117	25.867177967578876
29	21.56179187728778	23.880076695136832	28.743245598744988	25.8148858288304
30	22.311312532682585	24.21126024054384	30.88722328743246	22.59020393934112
31	22.346173958514903	24.751612340944746	26.407530067979778	26.494683632560573
32	23.025971762245074	25.78002440299808	29.196444134565102	21.997559700191736
33	20.707686944396027	24.681889489280113	30.608331880773925	24.00209168554994
34	23.147986752658184	26.930451455464528	26.63412933588984	23.28743245598745
35	22.032421126024055	24.838765905525538	30.224856196618443	22.903956771831968
36	23.914938120969147	25.431410144674913	24.943350183022485	25.710301551333448
37	22.29388181976643	25.10022659926791	27.993724943350184	24.61216663761548
38	22.450758236011854	23.339724594735927	28.202893498344082	26.00662367090814
39	23.70576956597525	22.311312532682585	30.277148335366917	23.70576956597525
40	21.422346173958516	21.126024054383823	32.07251176573122	25.379118005926443
41	20.951716925222243	22.48561966184417	29.283597699145897	27.27906571378769
42	21.05630120271919	21.021439776886876	33.31009238277846	24.61216663761548
43	19.086630643193306	22.747080355586544	32.543141014467494	25.62314798675266
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	2.5
4	5.0
5	5.0
6	4.5
7	4.0
8	7.0
9	10.0
10	12.0
11	14.0
12	14.0
13	36.5
14	59.0
15	103.5
16	148.0
17	110.5
18	73.0
19	73.0
20	91.5
21	110.0
22	77.5
23	45.0
24	41.5
25	38.0
26	38.0
27	44.5
28	51.0
29	67.5
30	84.0
31	85.5
32	87.0
33	87.0
34	93.5
35	100.0
36	111.0
37	122.0
38	149.5
39	177.0
40	177.0
41	210.0
42	243.0
43	273.5
44	304.0
45	365.5
46	427.0
47	427.0
48	496.0
49	565.0
50	568.0
51	571.0
52	493.5
53	416.0
54	416.0
55	490.5
56	565.0
57	498.0
58	431.0
59	351.5
60	272.0
61	272.0
62	243.5
63	215.0
64	185.5
65	156.0
66	154.5
67	153.0
68	153.0
69	130.0
70	107.0
71	83.0
72	59.0
73	49.5
74	40.0
75	40.0
76	40.5
77	41.0
78	29.5
79	18.0
80	16.0
81	14.0
82	14.0
83	10.0
84	6.0
85	4.5
86	3.0
87	2.5
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5737.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.19034338504446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.53416149068323	79.58863517517867
2	3.706004140786749	6.240195223984661
3	0.8695652173913043	2.196269827435942
4	0.2691511387163561	0.9063970716402301
5	0.12422360248447205	0.522921387484748
6	0.12422360248447205	0.6275056649816977
7	0.062111801242236024	0.36604497123932367
8	0.041407867494824016	0.2788914066585323
9	0.0	0.0
>10	0.2070393374741201	4.497123932368834
>50	0.041407867494824016	2.2834233920167333
>100	0.020703933747412008	2.4925919470106326
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	143	2.4925919470106326	RNA PCR Primer, Index 39 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	69	1.2027191912149207	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	62	1.0807042008018126	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	47	0.8192435070594387	No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC	46	0.8018127941432804	RNA PCR Primer, Index 39 (95% over 21bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC	36	0.6275056649816977	RNA PCR Primer, Index 39 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC	28	0.4880599616524316	No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG	23	0.4009063970716402	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.38347568415548194	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.31375283249084884	No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG	14	0.2440299808262158	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.20916855499389925	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	12	0.20916855499389925	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	8	0.13944570332926615	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	8	0.13944570332926615	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.1220149904131079	No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAA	7	0.1220149904131079	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	7	0.1220149904131079	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC	6	0.10458427749694962	No Hit
CTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCATA	6	0.10458427749694962	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC	6	0.10458427749694962	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	6	0.10458427749694962	No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTA	6	0.10458427749694962	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	6	0.10458427749694962	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.017430712916158272	0.0
23	0.0	0.0	0.0	0.034861425832316544	0.0
24	0.0	0.0	0.0	0.05229213874847481	0.0
25	0.0	0.0	0.0	0.06972285166463309	0.0
26	0.0	0.0	0.0	0.06972285166463309	0.0
27	0.0	0.0	0.0	0.08715356458079135	0.0
28	0.0	0.0	0.0	0.15687641624542445	0.0
29	0.0	0.0	0.0	0.2440299808262158	0.0
30	0.0	0.0	0.0	0.5403521004009064	0.0
31	0.0	0.0	0.0	0.9238277845563884	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCCGA	40	0.0017067059	23.125	1
CCGTCTT	45	0.0033850367	20.555555	37
CTCCGAG	45	0.0033850367	20.555555	2
AGCCCAC	50	0.0062316135	18.5	7
ATCTCGT	50	0.0062316135	18.5	27
GCCCACG	50	0.0062316135	18.5	8
CCACGAG	50	0.0062316135	18.5	10
GAGCCCA	50	0.0062316135	18.5	6
CCCACGA	50	0.0062316135	18.5	9
TCTACAT	50	0.0062316135	18.5	22
CTCTACA	50	0.0062316135	18.5	21
TACATCT	50	0.0062316135	18.5	24
ACGAGAC	50	0.0062316135	18.5	12
ACCTCTC	50	0.0062316135	18.5	17
CGAGCCC	50	0.0062316135	18.5	5
CCGAGCC	50	0.0062316135	18.5	4
AGACCTC	50	0.0062316135	18.5	15
TCTCTAC	50	0.0062316135	18.5	20
GCCGTCT	50	0.0062316135	18.5	36
CGAGACC	50	0.0062316135	18.5	13
>>END_MODULE