FastQCFastQC Report
Fri 10 Feb 2017
ERR1632116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2907794
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44010.15135184954642592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36000.12380519390300687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30290.10416831453672441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16400.025.0426851
CTTATAC18350.017.44141837
GTATCAA24400.016.8319682
GTATGTA2301.8189894E-1214.4782611
TCTTATA28350.013.76895937
TTAGAGT2355.638867E-1113.3829794
TAGAGTG3450.012.8695655
CAACGCA38400.012.863284
CTCTTAT44350.012.84780137
ATCAACG38500.012.829872
CGTCTAA1307.004988E-512.8076931
TCAACGC38500.012.7818183
GTATTAA3350.012.7014911
TATCAAC38550.012.669261
GTATTAT1901.0476106E-712.6578951
GTATTAG3850.012.4935061
GCTATAC1653.8086073E-612.3333323
AACGCAG42100.011.9524955
CTAACGT1552.7230397E-511.9354844
ATACCGT1257.224536E-411.846
Produced by FastQC (version 0.11.5)