FastQCFastQC Report
Fri 10 Feb 2017
ERR1632115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428153
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5400.12612313822395266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.11140877209782483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2800.029.7321411
CGCTCTT250.005492540529.59999828
CAATACG250.005492540529.5999984
TATGCCG400.001929218923.12500233
GCGTTAT400.001929218923.1250021
ATTCCGT400.001929218923.12500229
TATACTG400.001929218923.1250025
AACGTCT450.00382194720.5555556
ATACGAA450.00382194720.5555556
ACGAACG450.00382194720.55555515
AGAGTAA1001.2840246E-820.34999833
TAATAGG555.135853E-420.1818182
ATGCGTT555.135853E-420.18181836
CCGGAGT1700.019.58823624
AACCGAA609.2239154E-418.57
TTCCGTG500.007027851418.49999830
TACGAAT500.007027851418.4999987
CTCGATT500.007027851418.49999825
GTATCAA4500.018.0888882
CGGAGTC1603.274181E-1117.3437525
Produced by FastQC (version 0.11.5)