##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632115.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	428153
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.991874400039237	31.0	31.0	33.0	28.0	34.0
2	31.16129514449274	31.0	31.0	34.0	28.0	34.0
3	31.199213832438403	31.0	31.0	34.0	28.0	34.0
4	35.065616730467845	37.0	35.0	37.0	32.0	37.0
5	33.939645407132495	35.0	33.0	37.0	28.0	37.0
6	33.89153176551373	35.0	33.0	37.0	29.0	37.0
7	33.90057292603345	35.0	33.0	37.0	29.0	37.0
8	33.83893608126067	35.0	33.0	37.0	29.0	37.0
9	35.340523130749986	37.0	34.0	39.0	30.0	39.0
10	34.96458976113679	37.0	33.0	39.0	29.0	39.0
11	35.20272893101298	37.0	34.0	39.0	30.0	39.0
12	35.113571550357	37.0	34.0	39.0	29.0	39.0
13	35.242686609693266	37.0	34.0	39.0	30.0	39.0
14	36.112053401470966	38.0	34.0	40.0	30.0	41.0
15	36.045133398574805	38.0	34.0	40.0	30.0	41.0
16	35.930349664722655	38.0	34.0	40.0	29.0	41.0
17	35.937066889639915	38.0	34.0	40.0	29.0	41.0
18	35.93521941922631	38.0	34.0	40.0	29.0	41.0
19	35.997178578685656	38.0	34.0	40.0	29.0	41.0
20	35.96003998570605	38.0	34.0	40.0	29.0	41.0
21	35.89211566893143	38.0	34.0	40.0	29.0	41.0
22	35.87479709356235	38.0	34.0	40.0	29.0	41.0
23	35.7943515518985	38.0	34.0	40.0	29.0	41.0
24	35.69264491898924	38.0	34.0	40.0	28.0	41.0
25	35.577858849523416	38.0	34.0	40.0	27.0	41.0
26	35.38683834984223	38.0	34.0	40.0	27.0	41.0
27	35.25649709332879	38.0	34.0	40.0	27.0	41.0
28	35.11985435113149	38.0	33.0	40.0	27.0	41.0
29	34.92797201000577	37.0	33.0	39.0	26.0	40.0
30	34.829684715510574	37.0	33.0	39.0	26.0	40.0
31	34.695412621189156	37.0	33.0	39.0	25.0	40.0
32	34.56242044315934	37.0	33.0	39.0	25.0	40.0
33	34.377701429162	37.0	33.0	39.0	25.0	40.0
34	34.34473891342581	37.0	32.0	39.0	25.0	40.0
35	34.28046983204602	37.0	32.0	39.0	25.0	40.0
36	34.23770941696076	37.0	32.0	39.0	25.0	40.0
37	34.194977029239546	37.0	32.0	39.0	24.0	40.0
38	34.10035197698019	37.0	32.0	39.0	24.0	40.0
39	33.89962233126943	37.0	32.0	39.0	24.0	40.0
40	33.861026315359226	36.0	31.0	39.0	24.0	40.0
41	33.822647511520415	36.0	31.0	39.0	24.0	40.0
42	33.68317634116776	36.0	31.0	39.0	24.0	40.0
43	32.742423853155294	35.0	30.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	6.0
14	4.0
15	21.0
16	35.0
17	86.0
18	128.0
19	208.0
20	425.0
21	655.0
22	1078.0
23	1726.0
24	2550.0
25	3799.0
26	5399.0
27	7449.0
28	10044.0
29	13441.0
30	17598.0
31	22620.0
32	28378.0
33	35313.0
34	43001.0
35	51498.0
36	57768.0
37	58458.0
38	47762.0
39	18699.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.874983942656016	19.619388396204158	12.749180783504963	25.756446877634865
2	21.28281245255785	21.09596335889273	31.559279042772094	26.061945145777326
3	22.12153132174713	22.019698565699645	28.844595273185053	27.01417483936817
4	16.92829432469234	15.924447568976511	33.02604442804324	34.1212136782879
5	16.727431549002343	34.481598867694494	32.821911793214106	15.969057790089055
6	35.944860832459426	33.57935130665906	14.898412483387949	15.57737537749356
7	30.00913224945288	29.086097726747212	20.544758532580644	20.36001149121926
8	28.328658213302255	31.03750294871226	19.59393020719229	21.039908630793196
9	27.043136448886262	13.797170637599177	19.683851333518625	39.47584157999594
10	18.190927075134354	25.88467206816255	32.41061022578378	23.51379063091932
11	36.987478775110766	20.942279979353177	20.366551209497537	21.70369003603852
12	23.123743147893393	24.908852676496487	27.393712060875437	24.573692114734687
13	32.276546001079055	19.052301396930538	23.041529546680742	25.62962305530967
14	24.053550950244425	20.50949076615135	23.566809061246797	31.87014922235743
15	26.881044860131777	26.823588763829754	20.864737605482155	25.430628770556318
16	26.4125207577665	25.104343540743614	22.887612605774105	25.595523095715784
17	24.957433440849417	25.267602936333507	24.315606804109745	25.45935681870733
18	25.000642293759473	24.11357622158434	25.35705693992568	25.52872454473051
19	26.036019834031293	24.409264912309382	25.282550863826714	24.272164389832607
20	26.651921159024926	24.027392077131307	24.254880848668584	25.065805915175183
21	26.487026833865464	24.19905968193613	24.233393202897094	25.08052028130131
22	26.761695001553182	24.179206965734213	24.329153363400465	24.729944669312136
23	26.096745789472454	23.852221051820262	24.134129621887503	25.91690353681978
24	25.750140720723667	24.376566320917988	24.49428124992701	25.37901170843133
25	26.085301282485467	24.08344680523084	24.106102257837737	25.725149654445957
26	25.833755690138805	24.51226547519228	24.166594651911815	25.4873841827571
27	25.748272229787016	23.95078394872861	24.387543705170813	25.91340011631356
28	25.562123820223142	24.099095416825296	24.665481731997673	25.67329903095389
29	25.364297342305203	24.48867577711706	25.151756498261136	24.9952703823166
30	25.71417227019313	24.33312390664085	24.420942980663455	25.531760842502564
31	25.619112793791004	24.68533444819959	24.484238111142513	25.21131464686689
32	25.314782332483947	24.154683022190664	24.50736068648357	26.023173958841816
33	24.729944669312136	24.494981934028257	24.855367123434846	25.919706273224758
34	26.239451784759186	23.40331610428982	24.635819438378338	25.721412672572658
35	25.38368293577296	23.753424593544832	25.027268289606752	25.835624181075456
36	25.02493267593594	24.202329541075272	25.0153566598856	25.75738112310319
37	25.393258951823295	22.999021377871927	25.858980317783598	25.748739352521177
38	25.04198265573288	23.122108218323824	25.908728888971932	25.927180236971363
39	24.732513844350034	23.037559003440357	26.673875927530577	25.556051224679027
40	24.272631512566768	22.47280761783755	27.319205984776467	25.935354884819212
41	23.483427653198742	22.82852157990251	27.665811053525257	26.02223971337349
42	23.70998217926769	22.668532043451755	27.79497048952127	25.826515287759282
43	23.509820087678936	22.529329468671246	27.83210674688721	26.12874369676261
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	24.5
2	26.0
3	54.0
4	82.0
5	82.0
6	93.0
7	104.0
8	127.0
9	150.0
10	191.5
11	233.0
12	233.0
13	401.0
14	569.0
15	864.5
16	1160.0
17	1162.0
18	1164.0
19	1164.0
20	1671.0
21	2178.0
22	2407.0
23	2636.0
24	3363.0
25	4090.0
26	4090.0
27	4631.0
28	5172.0
29	6261.5
30	7351.0
31	8892.5
32	10434.0
33	10434.0
34	11803.5
35	13173.0
36	15135.0
37	17097.0
38	18110.0
39	19123.0
40	19123.0
41	20488.5
42	21854.0
43	23162.0
44	24470.0
45	25414.5
46	26359.0
47	26359.0
48	27563.0
49	28767.0
50	29391.5
51	30016.0
52	31250.0
53	32484.0
54	32484.0
55	31808.5
56	31133.0
57	30606.5
58	30080.0
59	29270.0
60	28460.0
61	28460.0
62	26720.5
63	24981.0
64	22739.5
65	20498.0
66	17966.0
67	15434.0
68	15434.0
69	13752.5
70	12071.0
71	10016.0
72	7961.0
73	5962.5
74	3964.0
75	3964.0
76	3031.5
77	2099.0
78	1637.0
79	1175.0
80	942.0
81	709.0
82	709.0
83	555.5
84	402.0
85	323.5
86	245.0
87	186.5
88	128.0
89	128.0
90	105.0
91	82.0
92	46.0
93	10.0
94	7.0
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	428153.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.8919871909378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.87630896996627	67.88923045559393
2	4.453909980873798	6.5821651851263345
3	1.1789598066337175	2.6134704879122745
4	0.6009338245871696	1.776167778759856
5	0.3986886844276827	1.4729949581451085
6	0.27193366822775406	1.205623147768196
7	0.20136919454736266	1.0415698960900228
8	0.1504962803209264	0.8896375374206144
9	0.10657166570887594	0.7087312941729451
>10	0.668692000543636	9.367498089033523
>50	0.06735453772622402	3.449127624900021
>100	0.02446367635404531	2.8770119065979793
>500	3.17710082520069E-4	0.1267716384792255
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	540	0.12612313822395266	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	477	0.11140877209782483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.335613670813938E-4	0.0
16	0.0	0.0	0.0	4.671227341627876E-4	0.0
17	0.0	0.0	0.0	9.342454683255751E-4	0.0
18	0.0	0.0	0.0	9.342454683255751E-4	0.0
19	0.0	0.0	0.0	0.001167806835406969	0.0
20	0.0	0.0	0.0	0.0014013682024883627	0.0
21	0.0	0.0	0.0	0.0018684909366511503	0.0
22	0.0	0.0	0.0	0.0028027364049767254	0.0
23	0.0	0.0	0.0	0.004204104607465089	0.0
24	0.0	0.0	0.0	0.005605472809953451	0.0
25	0.0	0.0	0.0	0.006773279645360421	0.0
26	0.0	0.0	0.0	0.009576016050337146	0.0
27	0.0	0.0	0.0	0.021721207138569624	0.0
28	0.0	0.0	0.0	0.057923219036185664	0.0
29	0.0	0.0	0.0	0.11748136764194109	0.0
30	0.0	0.0	0.0	0.1882504618676034	0.0
31	0.0	0.0	0.0	0.3804714669755905	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	280	0.0	29.732141	1
CGCTCTT	25	0.0054925405	29.599998	28
CAATACG	25	0.0054925405	29.599998	4
TATGCCG	40	0.0019292189	23.125002	33
GCGTTAT	40	0.0019292189	23.125002	1
ATTCCGT	40	0.0019292189	23.125002	29
TATACTG	40	0.0019292189	23.125002	5
AACGTCT	45	0.003821947	20.555555	6
ATACGAA	45	0.003821947	20.555555	6
ACGAACG	45	0.003821947	20.555555	15
AGAGTAA	100	1.2840246E-8	20.349998	33
TAATAGG	55	5.135853E-4	20.181818	2
ATGCGTT	55	5.135853E-4	20.181818	36
CCGGAGT	170	0.0	19.588236	24
AACCGAA	60	9.2239154E-4	18.5	7
TTCCGTG	50	0.0070278514	18.499998	30
TACGAAT	50	0.0070278514	18.499998	7
CTCGATT	50	0.0070278514	18.499998	25
GTATCAA	450	0.0	18.088888	2
CGGAGTC	160	3.274181E-11	17.34375	25
>>END_MODULE