FastQCFastQC Report
Fri 10 Feb 2017
ERR1632114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23377
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5392.3056850750737907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3691.5784745690208324No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT3011.287590366599649Illumina PCR Primer Index 11 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2981.274757240022244No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT1630.6972665440390128No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC1420.6074346579971768No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC1420.6074346579971768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.5261581896736108No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG790.33793899987166875No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC640.273773366984643Illumina PCR Primer Index 11 (95% over 23bp)
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG600.2566625315481028No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.22671856953415753No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA490.20960773409761732No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.19249689866107714No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT400.1711083543654019No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA380.1625529366471318No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA350.14971981006972668No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA320.1368866834923215No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT310.13260897463318647No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.12405355691491636No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA270.11549813919664628No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC260.11122043033751122No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG260.11122043033751122No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA260.11122043033751122No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGT260.11122043033751122No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA260.11122043033751122No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA250.10694272147837619No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT240.10266501261924114No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC240.10266501261924114No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCT240.10266501261924114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC303.4935516E-430.8333343
GGGAACA303.4935516E-430.83333421
CCGTCTT358.610815E-426.4285737
TACACAT551.8013518E-523.5454545
TTATACA400.00187541223.1252
GCCGTCT400.00187541223.12536
ATACACA400.00187541223.1254
TGGGAAC450.003716351720.55555720
GGTATCA3350.019.3283581
TGCCGTC500.00683551618.535
CATGGGC500.00683551618.516
TCTTATA701.1556158E-418.537
CTCTTAT803.2086115E-416.187536
ACACATC700.0024919815.8571426
GTATCAA4250.015.2352932
ACTGTCT750.003946562814.837
CATGGGG750.003946562814.818
CACATCT800.00605836513.8757
TGCTTCC850.00904892213.05882413
TCTCTTA1100.003560474411.77272735
Produced by FastQC (version 0.11.5)