##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632114.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23377
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0875647003465	33.0	31.0	34.0	30.0	34.0
2	32.22915686358387	33.0	31.0	34.0	30.0	34.0
3	32.038969927706724	34.0	31.0	34.0	30.0	34.0
4	35.84133977841468	37.0	35.0	37.0	35.0	37.0
5	35.68678615733413	37.0	35.0	37.0	35.0	37.0
6	35.80241262779655	37.0	35.0	37.0	35.0	37.0
7	35.786285665397614	37.0	35.0	37.0	35.0	37.0
8	35.81340633956453	37.0	35.0	37.0	35.0	37.0
9	37.472986268554564	39.0	37.0	39.0	35.0	39.0
10	37.353424305941736	39.0	37.0	39.0	35.0	39.0
11	37.506052958035674	39.0	37.0	39.0	35.0	39.0
12	37.40971895452795	39.0	37.0	39.0	35.0	39.0
13	37.483509432348036	39.0	37.0	39.0	35.0	39.0
14	38.4124566881978	40.0	38.0	41.0	34.0	41.0
15	38.4416306626171	40.0	38.0	41.0	34.0	41.0
16	38.21598152029773	40.0	38.0	41.0	34.0	41.0
17	38.45989647944561	40.0	38.0	41.0	34.0	41.0
18	38.67583522265475	40.0	38.0	41.0	35.0	41.0
19	38.779141891602855	40.0	38.0	41.0	35.0	41.0
20	38.7838473713479	40.0	38.0	41.0	35.0	41.0
21	38.824143388800955	40.0	38.0	41.0	35.0	41.0
22	38.68456174872738	40.0	38.0	41.0	35.0	41.0
23	38.5984086923044	40.0	38.0	41.0	35.0	41.0
24	38.47867562133721	40.0	38.0	41.0	35.0	41.0
25	38.43316079907601	40.0	38.0	41.0	34.0	41.0
26	38.29007143773795	40.0	38.0	41.0	34.0	41.0
27	38.127689609445184	40.0	37.0	41.0	34.0	41.0
28	38.01480087265261	40.0	37.0	41.0	33.0	41.0
29	37.76348547717842	40.0	37.0	41.0	33.0	41.0
30	37.556444368396285	39.0	36.0	41.0	33.0	41.0
31	37.309706121401376	39.0	35.0	40.0	33.0	41.0
32	37.08474141249947	39.0	35.0	40.0	32.0	41.0
33	36.83077383753262	39.0	35.0	40.0	31.0	41.0
34	36.72785216238183	39.0	35.0	40.0	31.0	41.0
35	36.640586901655475	39.0	35.0	40.0	31.0	41.0
36	36.564400906874276	39.0	35.0	40.0	31.0	41.0
37	36.36168028403987	39.0	35.0	40.0	31.0	41.0
38	36.19450742182487	39.0	35.0	40.0	30.0	41.0
39	35.90251101510031	38.0	35.0	40.0	29.0	41.0
40	35.78038242717201	38.0	35.0	40.0	29.0	41.0
41	35.58005732129871	38.0	35.0	40.0	28.0	41.0
42	35.25751807331993	38.0	35.0	40.0	26.0	41.0
43	34.37070624973264	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	0.0
20	0.0
21	9.0
22	9.0
23	29.0
24	40.0
25	56.0
26	88.0
27	142.0
28	205.0
29	301.0
30	363.0
31	509.0
32	654.0
33	790.0
34	1094.0
35	1500.0
36	2501.0
37	4283.0
38	6277.0
39	4523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21012105916071	15.570860247251572	20.109509346793857	20.109509346793857
2	20.669889207340546	22.62052444710613	32.31809043076528	24.391495914788038
3	18.8818069042221	23.364845788595627	38.94853916242461	18.80480814475767
4	12.482354450956068	19.989733498738076	30.47439791247808	37.05351413782778
5	11.998973349873808	36.835350986011896	33.72117893656158	17.44449672755272
6	28.66492706506395	34.516832784360695	15.506694614364546	21.311545536210804
7	20.83244214398768	36.6043547076186	18.466869144886	24.09633400350772
8	32.81858236728408	29.430636950849127	17.482996107284936	20.267784574581853
9	25.51225563588142	10.955212388244856	17.21350044915943	46.31903152671429
10	21.692261624673826	26.68862557214356	23.59584206698892	28.023270736193695
11	32.6988065192283	22.731744877443642	16.024297386319887	28.54515121700817
12	20.464559182102064	35.70603584720024	23.411900586046112	20.41750438465158
13	37.65239337810669	17.748214056551312	23.63861915558027	20.96077340976173
14	24.00222440860675	23.488899345510543	26.6201822303974	25.88869401548531
15	30.414509988450185	25.27270393976986	22.804465928048938	21.50832014373102
16	21.486931599435344	23.75839500363605	27.84788467296916	26.906788723959448
17	17.008170423920948	34.23878170851692	23.711340206185564	25.041707661376567
18	18.997305043418745	20.58861273901698	30.119348077169867	30.294734140394404
19	22.641912991401806	26.851178508790692	31.1117765324892	19.395131967318306
20	25.92291568635839	20.828164435128546	31.975873722034475	21.27304615647859
21	32.44642169653933	19.27107841040339	26.975232065705608	21.30726782735167
22	30.423065406168455	23.548787269538433	25.798862129443474	20.229285194849638
23	24.7893228386876	23.450399965778328	27.835051546391753	23.92522564914232
24	22.175642725756084	27.142062711211874	30.50861958335116	20.173674979680882
25	21.563930358899775	25.60636523078239	27.659665483167217	25.170038927150618
26	22.62052444710613	22.582025067373916	29.14403045728708	25.653420028232876
27	22.53497026992343	25.388202078966504	27.826496128673483	24.250331522436582
28	20.254951448004448	23.454677674637463	32.62180775976387	23.668563117594214
29	22.928519484963854	23.403345168327842	32.668862557214354	20.999272789493947
30	22.40663900414938	23.980835864311075	32.202592291568635	21.40993283997091
31	22.56919194079651	24.583992813449115	29.182529837019295	23.66428540873508
32	21.914702485348847	25.353980408093424	32.33520126620182	20.396115840355904
33	18.586644992941782	24.79787825640587	34.722162809599176	21.893313941053172
34	20.490225435256875	26.50896180005989	30.607006887111265	22.393805877571975
35	19.01013816999615	26.222355306497843	32.63036317748214	22.13714334602387
36	19.134191726911066	26.74851349617145	29.06703169782265	25.050263079094837
37	18.12893014501433	27.753775078068188	30.448731659323265	23.668563117594214
38	18.911750866236044	24.549771142576034	30.812336912349746	25.72614107883817
39	18.84758523334902	24.50271634512555	32.322368139624416	24.327330281901013
40	18.252983701929246	23.06968387731531	34.35427984771356	24.323052573041878
41	17.67121529708688	24.12200025666253	34.2858365059674	23.920947940283185
42	17.59421653762245	22.94135261154126	35.7744791889464	23.68995166188989
43	16.182572614107883	24.194721307267827	35.44937331565214	24.17333276297215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	10.5
4	21.0
5	21.0
6	37.0
7	53.0
8	62.0
9	71.0
10	105.5
11	140.0
12	140.0
13	248.5
14	357.0
15	651.0
16	945.0
17	785.5
18	626.0
19	626.0
20	662.5
21	699.0
22	486.0
23	273.0
24	265.5
25	258.0
26	258.0
27	267.5
28	277.0
29	280.5
30	284.0
31	304.0
32	324.0
33	324.0
34	298.5
35	273.0
36	328.0
37	383.0
38	461.5
39	540.0
40	540.0
41	708.0
42	876.0
43	1081.0
44	1286.0
45	1705.0
46	2124.0
47	2124.0
48	2526.0
49	2928.0
50	2549.0
51	2170.0
52	2014.5
53	1859.0
54	1859.0
55	1474.0
56	1089.0
57	1332.0
58	1575.0
59	1337.5
60	1100.0
61	1100.0
62	909.0
63	718.0
64	649.0
65	580.0
66	523.0
67	466.0
68	466.0
69	437.0
70	408.0
71	348.5
72	289.0
73	229.0
74	169.0
75	169.0
76	141.5
77	114.0
78	80.5
79	47.0
80	40.0
81	33.0
82	33.0
83	22.5
84	12.0
85	9.5
86	7.0
87	4.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	23377.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.9866107712709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.3673342841198	60.96162895153356
2	6.634835989334656	9.154296958549
3	2.108265641470825	4.363263036317748
4	0.9549203199603149	2.635068657227189
5	0.4712593786817139	1.6255293664713182
6	0.3472437527128418	1.4373101766693759
7	0.1736218763564209	0.8384309363904693
8	0.1426179698642029	0.7870984300808487
9	0.06820859428287963	0.4234931770543697
>10	0.657282817635022	7.793985541344056
>50	0.024803125193774415	1.095093467938572
>100	0.043405469089105225	6.579116225349703
>500	0.006200781298443604	2.3056850750737907
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	539	2.3056850750737907	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	369	1.5784745690208324	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	301	1.287590366599649	Illumina PCR Primer Index 11 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	298	1.274757240022244	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	163	0.6972665440390128	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	142	0.6074346579971768	No Hit
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	142	0.6074346579971768	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	123	0.5261581896736108	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	79	0.33793899987166875	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	64	0.273773366984643	Illumina PCR Primer Index 11 (95% over 23bp)
CACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCG	60	0.2566625315481028	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.22671856953415753	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	49	0.20960773409761732	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.19249689866107714	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCT	40	0.1711083543654019	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	38	0.1625529366471318	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	35	0.14971981006972668	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.1368866834923215	No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCCTGTCT	31	0.13260897463318647	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29	0.12405355691491636	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	27	0.11549813919664628	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATAC	26	0.11122043033751122	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	26	0.11122043033751122	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	26	0.11122043033751122	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGT	26	0.11122043033751122	No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA	26	0.11122043033751122	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	25	0.10694272147837619	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCT	24	0.10266501261924114	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATAC	24	0.10266501261924114	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCT	24	0.10266501261924114	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.012833126577405141	0.0
18	0.0	0.0	0.0	0.01711083543654019	0.0
19	0.0	0.0	0.0	0.021388544295675237	0.0
20	0.0	0.0	0.0	0.025666253154810283	0.0
21	0.0	0.0	0.0	0.05561021516875561	0.0
22	0.0	0.0	0.0	0.0812764683235659	0.0
23	0.0	0.0	0.0	0.11549813919664628	0.0
24	0.0	0.0	0.0	0.17538606322453693	0.0
25	0.0	0.0	0.004277708859135048	0.19677460752021217	0.0
26	0.0	0.0	0.004277708859135048	0.2181631518158874	0.0
27	0.0	0.0	0.004277708859135048	0.24810711382983275	0.0
28	0.0	0.0	0.004277708859135048	0.32082816443512857	0.0
29	0.0	0.0	0.004277708859135048	0.5817684048423665	0.0
30	0.0	0.0	0.004277708859135048	1.0694272147837618	0.0
31	0.0	0.0	0.004277708859135048	2.066133378962228	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	30	3.4935516E-4	30.833334	3
GGGAACA	30	3.4935516E-4	30.833334	21
CCGTCTT	35	8.610815E-4	26.42857	37
TACACAT	55	1.8013518E-5	23.545454	5
TTATACA	40	0.001875412	23.125	2
GCCGTCT	40	0.001875412	23.125	36
ATACACA	40	0.001875412	23.125	4
TGGGAAC	45	0.0037163517	20.555557	20
GGTATCA	335	0.0	19.328358	1
TGCCGTC	50	0.006835516	18.5	35
CATGGGC	50	0.006835516	18.5	16
TCTTATA	70	1.1556158E-4	18.5	37
CTCTTAT	80	3.2086115E-4	16.1875	36
ACACATC	70	0.00249198	15.857142	6
GTATCAA	425	0.0	15.235293	2
ACTGTCT	75	0.0039465628	14.8	37
CATGGGG	75	0.0039465628	14.8	18
CACATCT	80	0.006058365	13.875	7
TGCTTCC	85	0.009048922	13.058824	13
TCTCTTA	110	0.0035604744	11.772727	35
>>END_MODULE