FastQCFastQC Report
Fri 10 Feb 2017
ERR1632113.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632113.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307955
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT6190.20100339335292497RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT3720.12079686967251708No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC3440.11170463217028462No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC3090.10033933529249403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG200.001839844937.010
AAGACTG352.3826753E-531.7142855
TGTATAC250.005490173629.68
GTATACG250.005490173629.69
GTCCATA250.005490173629.61
TAAGCTT250.005490173629.64
GTTACAC405.9262333E-527.753
GGTATCA1550.027.4516121
CCGTCTT905.456968E-1226.72222137
GCCGTCT1059.749783E-1021.14285936
TTTATGT450.003819546720.5555554
TACCAAT500.007023476518.523
TCGTGTA500.007023476518.513
GTATCAA2400.018.52
GCCTACA609.215899E-418.51
TATGCCG1258.507413E-917.7633
TGCCGTC1258.507413E-917.7635
TACAGAG650.001576385817.0769235
ATGCCGT1301.38152245E-817.07692334
ATAAAAC700.002586719615.8571423
Produced by FastQC (version 0.11.5)