FastQCFastQC Report
Fri 10 Feb 2017
ERR1632112.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632112.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2606201
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58320.22377399133835033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57310.21989861871743585No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42250.16211335963726511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21700.024.0414751
GTATCAA31700.016.5741332
CGAATTA803.384624E-416.187515
CTTATAC26050.016.1209237
TCTTATA45000.015.99222237
TATATCG800.006301447313.8755
TATACTG2950.013.7966095
CAAGACG5850.013.2820524
ACGGACC4800.013.1041668
CTCTTAT69900.012.99499337
GACGGAC5000.012.957
GCCGTTA1000.001833564312.94999915
TAGGACG1153.5798785E-412.8695644
AAGACGG5600.012.5535715
CGAAAGC5450.012.22018319
ATCAACG43550.012.1492544
GCGAAAG5350.012.10280418
TATACAC11050.012.05429837
ATAGGAC6450.012.0465123
TCTCTTA104650.011.98566637
Produced by FastQC (version 0.11.5)