FastQCFastQC Report
Fri 10 Feb 2017
ERR1632111.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632111.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455324
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5460.11991461025555429No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA5140.11288664774973425No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG5100.11200815243650675No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC4730.10388207078915233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTC250.00549290229.5999988
ATAGGAC451.3214983E-424.6666663
GGTATCA2750.023.5454561
TCGTTCT450.003822313420.55555530
AGGACCG450.003822313420.5555555
CGGTCCA555.13654E-420.18181810
GACTGAG902.146413E-618.57
CGCGGTT500.00702851918.49999810
CGGACCA500.00702851918.4999989
CTTATAC3450.017.69565237
ATCCTAC1054.781559E-717.61904725
ACCTCTA752.0638134E-417.26666625
CGGCCTT1301.3866156E-817.07692324
TATACTT650.00157795417.0769235
TCTATGG650.00157795417.0769232
ATTGTTT1107.782164E-716.81818217
GTATCAA3950.015.924052
GCTTAGG1403.4566256E-815.8571421
ATCTCGC1302.578745E-715.65384611
AATTGTT957.044716E-515.57894816
Produced by FastQC (version 0.11.5)