##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632111.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455324
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.852373255088686	34.0	31.0	34.0	31.0	34.0
2	33.017594064885664	34.0	33.0	34.0	31.0	34.0
3	33.096419253103285	34.0	33.0	34.0	31.0	34.0
4	36.46519840816649	37.0	37.0	37.0	35.0	37.0
5	36.342938215424624	37.0	37.0	37.0	35.0	37.0
6	36.37800115961382	37.0	37.0	37.0	35.0	37.0
7	36.35622106456062	37.0	37.0	37.0	35.0	37.0
8	36.34075295833297	37.0	37.0	37.0	35.0	37.0
9	38.14174961126582	39.0	39.0	39.0	37.0	39.0
10	38.12615851569432	39.0	38.0	39.0	37.0	39.0
11	38.18709753933463	39.0	39.0	39.0	37.0	39.0
12	38.16645948818863	39.0	39.0	39.0	37.0	39.0
13	38.16908618917518	39.0	39.0	39.0	37.0	39.0
14	39.60649339810772	41.0	39.0	41.0	37.0	41.0
15	39.62701504862471	41.0	39.0	41.0	37.0	41.0
16	39.61326659697271	41.0	39.0	41.0	37.0	41.0
17	39.60287838989379	41.0	39.0	41.0	37.0	41.0
18	39.594113202906065	41.0	39.0	41.0	37.0	41.0
19	39.62629468246787	41.0	39.0	41.0	37.0	41.0
20	39.608169127917705	41.0	39.0	41.0	37.0	41.0
21	39.5828486967522	41.0	39.0	41.0	37.0	41.0
22	39.55485544359621	41.0	39.0	41.0	37.0	41.0
23	39.507010392599554	41.0	39.0	41.0	37.0	41.0
24	39.467229928578334	41.0	39.0	41.0	37.0	41.0
25	39.41844049511996	41.0	39.0	41.0	37.0	41.0
26	39.34876703182788	40.0	39.0	41.0	36.0	41.0
27	39.28254825135508	40.0	39.0	41.0	36.0	41.0
28	39.21976878003356	40.0	39.0	41.0	36.0	41.0
29	39.17769324700653	40.0	39.0	41.0	36.0	41.0
30	39.11271314492537	40.0	39.0	41.0	36.0	41.0
31	39.051236042905714	40.0	39.0	41.0	35.0	41.0
32	38.981230947632895	40.0	39.0	41.0	35.0	41.0
33	38.919740668183536	40.0	38.0	41.0	35.0	41.0
34	38.84540019853994	40.0	38.0	41.0	35.0	41.0
35	38.8284254728501	40.0	38.0	41.0	35.0	41.0
36	38.80547039031547	40.0	38.0	41.0	35.0	41.0
37	38.753847809471935	40.0	38.0	41.0	35.0	41.0
38	38.70265788757017	40.0	38.0	41.0	35.0	41.0
39	38.65728799711854	40.0	38.0	41.0	35.0	41.0
40	38.60677890908452	40.0	38.0	41.0	35.0	41.0
41	38.56805483567745	40.0	38.0	41.0	35.0	41.0
42	38.53331913099244	40.0	38.0	41.0	35.0	41.0
43	37.69274626419868	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	4.0
19	6.0
20	20.0
21	29.0
22	72.0
23	143.0
24	236.0
25	356.0
26	551.0
27	769.0
28	1111.0
29	1629.0
30	2241.0
31	3301.0
32	4568.0
33	6352.0
34	9566.0
35	14552.0
36	23490.0
37	43250.0
38	111052.0
39	232021.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.23476030255379	18.148175804482083	12.82800818757632	26.78905570538781
2	19.432755576249	20.359568131704016	34.12449157083747	26.083184721209513
3	20.653424813978617	21.366543384491045	29.43684057945551	28.54319122207483
4	16.066361535961203	15.607347734799834	32.403080004568174	35.92321072467078
5	16.34176981665803	34.464469257056514	33.06085337034727	16.13290755593819
6	35.88126257346417	32.65872214071738	15.378938953360684	16.081076332457766
7	29.865107044653914	28.959817624372974	20.60554681940772	20.56952851156539
8	26.72382742838067	32.90118684716817	20.011464363837618	20.36352136061354
9	26.57052999622247	14.598835115214662	19.409914698105084	39.420720190457786
10	17.9347014433678	26.270084599098663	32.32269768340786	23.472516274125677
11	36.51312032750305	20.994720243167503	20.81353058481433	21.678628844515114
12	22.255800265305584	24.541864694151858	27.75496130228145	25.44737373826111
13	30.76314887860073	19.163716386573075	23.908469573314825	26.164665161511362
14	23.836872205286785	20.43951120520772	23.329760785726208	32.39385580377929
15	26.386045980444695	26.97200235436744	21.318445766091838	25.323505899096027
16	26.375504036685964	25.4631866538992	22.74753801688468	25.41377129253015
17	25.079723449675395	25.565970605546816	24.079336911737574	25.274969033040207
18	24.380880428002914	24.47048694995212	25.51545712503624	25.633175497008725
19	25.743426658818773	24.80673103108995	25.092900879373808	24.356941430717466
20	25.94723757148756	24.01432825855874	25.26113273185688	24.777301438096828
21	25.715973680280417	24.82078695610159	23.97413709797858	25.489102265639414
22	25.972274687914542	24.146761427027787	24.024650578489165	25.856313306568506
23	25.088069155151054	24.778399557238362	24.425903312805826	25.707627974804755
24	26.176305224411628	24.703947079442333	24.028823431226996	25.090924264919046
25	25.86114503079126	23.938777661621174	24.420851964754767	25.779225342832795
26	25.75726296000211	24.494425947237573	24.290395410740484	25.457915682019838
27	25.90682678707909	23.85048888264181	24.152691270392072	26.089993059887025
28	24.99780376171693	24.405258672944978	24.73996538728466	25.85697217805343
29	24.899851534292065	24.749409211901856	24.916103697586774	25.43463555621931
30	24.73974576345635	25.49371436603386	24.676933348560585	25.089606521949204
31	25.618899948168778	24.45730952025371	24.64772337939577	25.276067152181742
32	24.63871880243519	24.296764501761384	24.921155045637832	26.1433616501656
33	24.902706644060054	24.0417812370971	24.86229585965159	26.19321625919126
34	25.38609869016349	23.86059157874393	24.885136737795506	25.86817299329708
35	25.21018000368968	23.757588003268	25.235436743944973	25.79679524909735
36	24.66683065245847	24.020477725751334	25.588811483690733	25.723880138099464
37	25.511284272298408	22.43018158498124	25.771538508842056	26.28699563387829
38	24.204742117700803	22.81935500874103	27.320545369890453	25.65535750366772
39	24.12589716333863	22.40031274433151	27.697200235436743	25.77658985689311
40	23.53840342261774	22.16619374335638	28.528915673234884	25.766487160790994
41	22.818915761084416	22.546142966327274	28.909304143862393	25.72563712872592
42	23.314167493916422	22.182006658994474	28.478182568895992	26.02564327819311
43	22.189693492985217	22.562395129621983	28.9158928587116	26.332018518681206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	17.5
2	19.0
3	37.5
4	56.0
5	56.0
6	67.5
7	79.0
8	87.0
9	95.0
10	154.0
11	213.0
12	213.0
13	339.5
14	466.0
15	756.0
16	1046.0
17	1124.5
18	1203.0
19	1203.0
20	1487.0
21	1771.0
22	2135.5
23	2500.0
24	3077.5
25	3655.0
26	3655.0
27	4187.0
28	4719.0
29	6525.0
30	8331.0
31	9296.0
32	10261.0
33	10261.0
34	11574.5
35	12888.0
36	14521.0
37	16154.0
38	19151.0
39	22148.0
40	22148.0
41	24435.5
42	26723.0
43	26508.5
44	26294.0
45	28393.0
46	30492.0
47	30492.0
48	32094.0
49	33696.0
50	36062.0
51	38428.0
52	40786.5
53	43145.0
54	43145.0
55	38282.5
56	33420.0
57	31803.0
58	30186.0
59	27846.5
60	25507.0
61	25507.0
62	23609.5
63	21712.0
64	19623.0
65	17534.0
66	15585.5
67	13637.0
68	13637.0
69	11699.0
70	9761.0
71	8626.5
72	7492.0
73	6053.5
74	4615.0
75	4615.0
76	3713.0
77	2811.0
78	2327.5
79	1844.0
80	1461.0
81	1078.0
82	1078.0
83	846.0
84	614.0
85	496.0
86	378.0
87	298.0
88	218.0
89	218.0
90	156.5
91	95.0
92	56.0
93	17.0
94	11.5
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	455324.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.45244883268883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.32328773814984	58.215570813433324
2	5.3399836181270155	6.8835004182945605
3	1.4139064637428072	2.7338920202577386
4	0.6896363076748235	1.7779499533430405
5	0.39542722426868837	1.27431264696149
6	0.2578682348496251	0.9972143527332747
7	0.19669328221640214	0.8874154595450398
8	0.1550870350715788	0.799659135405148
9	0.11460458889976662	0.664789176184819
>10	0.909460991336186	11.884516706069778
>50	0.13387227826778564	5.961277674911501
>100	0.06915031160817488	7.575204900718234
>500	0.0010219257873129292	0.34469674214206386
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	546	0.11991461025555429	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	514	0.11288664774973425	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	510	0.11200815243650675	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	473	0.10388207078915233	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.1962382830687598E-4	0.0
12	0.0	0.0	0.0	2.1962382830687598E-4	0.0
13	0.0	0.0	0.0	2.1962382830687598E-4	0.0
14	0.0	0.0	0.0	2.1962382830687598E-4	0.0
15	0.0	0.0	0.0	2.1962382830687598E-4	0.0
16	0.0	0.0	0.0	2.1962382830687598E-4	0.0
17	0.0	0.0	0.0	4.3924765661375195E-4	0.0
18	0.0	0.0	0.0	6.58871484920628E-4	0.0
19	0.0	0.0	0.0	8.784953132275039E-4	0.0
20	0.0	0.0	0.0	0.00109811914153438	0.0
21	0.0	0.0	0.0	0.00109811914153438	0.0
22	0.0	0.0	0.0	0.00109811914153438	0.0
23	0.0	0.0	0.0	0.0015373667981481319	0.0
24	0.0	0.0	0.0	0.0024158621113756357	0.0
25	0.0	0.0	0.0	0.00329435742460314	0.0
26	0.0	0.0	0.0	0.007906457819047535	0.0
27	0.0	0.0	0.0	0.02679410705343887	0.0
28	0.0	0.0	0.0	0.08894765046428477	0.0
29	0.0	0.0	0.0	0.17394207201904577	0.0
30	0.0	0.0	0.0	0.26925881350423	0.0
31	0.0	0.0	0.0	0.5943020793984064	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGGTC	25	0.005492902	29.599998	8
ATAGGAC	45	1.3214983E-4	24.666666	3
GGTATCA	275	0.0	23.545456	1
TCGTTCT	45	0.0038223134	20.555555	30
AGGACCG	45	0.0038223134	20.555555	5
CGGTCCA	55	5.13654E-4	20.181818	10
GACTGAG	90	2.146413E-6	18.5	7
CGCGGTT	50	0.007028519	18.499998	10
CGGACCA	50	0.007028519	18.499998	9
CTTATAC	345	0.0	17.695652	37
ATCCTAC	105	4.781559E-7	17.619047	25
ACCTCTA	75	2.0638134E-4	17.266666	25
CGGCCTT	130	1.3866156E-8	17.076923	24
TATACTT	65	0.001577954	17.076923	5
TCTATGG	65	0.001577954	17.076923	2
ATTGTTT	110	7.782164E-7	16.818182	17
GTATCAA	395	0.0	15.92405	2
GCTTAGG	140	3.4566256E-8	15.857142	1
ATCTCGC	130	2.578745E-7	15.653846	11
AATTGTT	95	7.044716E-5	15.578948	16
>>END_MODULE