FastQCFastQC Report
Fri 10 Feb 2017
ERR1632110.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632110.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321902
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT3480.10810743642475039RNA PCR Primer, Index 26 (100% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGT250.005490539629.66
GGCCATA250.005490539629.611
TCGTTTA453.9964834E-628.77777730
CGTTTAT453.9964834E-628.77777731
GTCCTAT601.3324152E-624.6666661
TCCTATT551.897147E-523.5454542
ATGGTCG502.6962504E-422.236
CCTATTC705.083115E-621.1428593
GTACAGA450.003819917320.5555551
TATGGTC555.1320455E-420.18181835
GTTTATG656.882419E-519.92307732
ATCGCCG656.882419E-519.92307716
CTATTCC759.231833E-619.7333344
CTTATAC2850.018.8245637
ATTCCAT701.2157401E-418.56
GGTATCA2100.018.51
TTTATGG701.2157401E-418.533
GTCTTGG500.00702415318.51
TCCATTA752.0614469E-417.2666668
GCCGCAT752.0614469E-417.26666611
Produced by FastQC (version 0.11.5)