##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632110.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	321902
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94517586097632	33.0	31.0	34.0	30.0	34.0
2	32.14564059869153	33.0	31.0	34.0	30.0	34.0
3	32.23016943044777	34.0	31.0	34.0	30.0	34.0
4	35.877810638020264	37.0	35.0	37.0	35.0	37.0
5	35.50882566743916	37.0	35.0	37.0	33.0	37.0
6	35.4977104833148	37.0	35.0	37.0	33.0	37.0
7	35.47508558505383	37.0	35.0	37.0	33.0	37.0
8	35.453013650117114	37.0	35.0	37.0	33.0	37.0
9	37.14997732229064	39.0	37.0	39.0	34.0	39.0
10	36.99311281073122	39.0	37.0	39.0	33.0	39.0
11	37.12342265658492	39.0	37.0	39.0	33.0	39.0
12	37.02810482693491	39.0	37.0	39.0	33.0	39.0
13	37.0928791992594	39.0	37.0	39.0	33.0	39.0
14	38.25339699660145	40.0	38.0	41.0	34.0	41.0
15	38.226071910084436	40.0	38.0	41.0	34.0	41.0
16	38.223810352219	40.0	38.0	41.0	34.0	41.0
17	38.17023504047816	40.0	38.0	41.0	33.0	41.0
18	38.16209902392654	40.0	38.0	41.0	33.0	41.0
19	38.187991997564474	40.0	38.0	41.0	34.0	41.0
20	38.14095283657759	40.0	38.0	41.0	34.0	41.0
21	38.10540785704966	40.0	38.0	41.0	34.0	41.0
22	38.049946878242444	40.0	37.0	41.0	33.0	41.0
23	37.99334269436039	40.0	37.0	41.0	33.0	41.0
24	37.92784760579307	40.0	37.0	41.0	33.0	41.0
25	37.85083659001808	39.0	37.0	41.0	33.0	41.0
26	37.71258954588664	39.0	37.0	40.0	33.0	41.0
27	37.59670023796062	39.0	37.0	40.0	33.0	41.0
28	37.51848388640021	39.0	37.0	40.0	32.0	41.0
29	37.425051723816566	39.0	37.0	40.0	32.0	41.0
30	37.34434082422601	39.0	36.0	40.0	32.0	41.0
31	37.23951699585588	39.0	36.0	40.0	31.0	41.0
32	37.13101502941889	39.0	36.0	40.0	31.0	41.0
33	37.02470938360122	39.0	36.0	40.0	31.0	41.0
34	36.94125541313816	39.0	36.0	40.0	31.0	41.0
35	36.91499276177222	39.0	36.0	40.0	31.0	41.0
36	36.87708992177743	39.0	36.0	40.0	31.0	41.0
37	36.796733167237235	39.0	35.0	40.0	31.0	41.0
38	36.737929556200335	39.0	35.0	40.0	31.0	41.0
39	36.66738634739765	39.0	35.0	40.0	30.0	41.0
40	36.584833272238136	38.0	35.0	40.0	30.0	41.0
41	36.54438307310921	38.0	35.0	40.0	30.0	41.0
42	36.48467856676877	38.0	35.0	40.0	30.0	41.0
43	35.49899969555952	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	2.0
16	2.0
17	8.0
18	14.0
19	32.0
20	58.0
21	104.0
22	192.0
23	325.0
24	565.0
25	776.0
26	1259.0
27	1868.0
28	2598.0
29	3678.0
30	4958.0
31	6660.0
32	8610.0
33	11775.0
34	16494.0
35	24048.0
36	37357.0
37	59894.0
38	91860.0
39	48763.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.896906511919774	17.881529161049016	12.12045902168983	27.101105305341378
2	21.066349385837928	19.508732471373275	31.573895160638955	27.851022982149846
3	21.42018378264192	20.41553019241881	28.17348137010643	29.990804654832836
4	16.609713515293475	14.966045566663148	31.414219234425385	37.01002168361799
5	17.89830445290803	33.11970724009171	31.818690160359363	17.163298146640905
6	38.017160502264666	31.821175388782923	14.300004349149742	15.861659759802674
7	31.724251480264183	27.83020919410255	19.39379065678374	21.051748668849527
8	29.141788494635012	30.935502109337627	18.61777808152792	21.304931314499445
9	27.45742493056893	13.297214680244299	18.149933830793223	41.09542655839355
10	18.718429832681995	24.814073848562607	31.041745624444705	25.42575069431069
11	39.326254574373564	19.466794241725744	19.23659995899373	21.97035122490696
12	23.68391622294984	23.260495430286234	26.855999652068018	26.1995886946959
13	33.13896776037428	17.55627489111593	21.788929549987262	27.51582779852253
14	24.606557275195556	18.83741014345981	22.324185621710956	34.23184695963368
15	27.759380184031162	25.153618181931147	19.77962236954104	27.30737926449665
16	27.834247690290837	23.408987828593798	21.641058458785594	27.11570602232978
17	26.367341613286033	23.8324086212574	22.90666103348224	26.893588731974326
18	26.855378344962133	22.617753229243682	23.492242980782972	27.034625445011212
19	27.301166193437755	23.040242061248453	23.275406800827582	26.38318494448621
20	27.436921796074582	22.49939422557176	23.11262433908457	26.951059639269094
21	28.094264714105538	22.63080067846736	22.59041571658455	26.684518890842558
22	28.12967921914123	22.65658492336177	22.40806208100602	26.80567377649098
23	27.518623680499033	22.481065665948023	22.62614087517319	27.374169778379752
24	27.18125392200111	23.10237277183739	22.371404961758547	27.344968344402954
25	27.483519829016284	22.249007461898344	22.606880354890617	27.66059235419476
26	27.21325123795441	22.75816863517468	22.86161626830526	27.16696385856565
27	27.259849270896115	22.591347677243384	22.824027188398954	27.324775863461547
28	26.970941466657557	22.568359314325477	23.276960068592302	27.183739150424664
29	26.66339444924232	22.89609881268212	23.503737162241926	26.93676957583364
30	26.705022025336906	23.0405527148014	23.23160464986238	27.022820609999314
31	27.238414175742925	22.825891109716622	22.69510596392691	27.240588750613544
32	26.514902050934758	22.832725487881405	23.20706301917975	27.44530944200409
33	26.262651365943672	22.445651160912327	23.787053202527478	27.504644270616524
34	26.876192133009425	22.40526619902952	23.41209436412324	27.306447303837817
35	26.554044398605786	22.30119725879305	24.18313648253195	26.96162186006921
36	26.292474107026365	22.285043274039925	23.95635938888233	27.46612323005138
37	26.00014911370541	21.630806891538416	24.759088169691395	27.60995582506477
38	25.378220700710152	21.47765468993669	25.858180440009694	27.285944169343463
39	25.276015681791353	21.005461289460765	26.170076607166155	27.548446421581723
40	24.874961944939763	21.124441600238583	27.002317475504967	26.998278979316687
41	24.426067560934694	21.148361923815322	27.41766127579201	27.007909239457973
42	24.121005771943015	21.146498002497655	27.554348839087673	27.178147386471657
43	23.480438145771075	20.672440680704067	28.0147374045517	27.832383768973166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	6.5
2	6.0
3	14.5
4	23.0
5	23.0
6	26.0
7	29.0
8	30.5
9	32.0
10	51.5
11	71.0
12	71.0
13	144.5
14	218.0
15	306.5
16	395.0
17	402.0
18	409.0
19	409.0
20	542.5
21	676.0
22	691.0
23	706.0
24	914.5
25	1123.0
26	1123.0
27	1433.5
28	1744.0
29	2330.5
30	2917.0
31	3549.5
32	4182.0
33	4182.0
34	5235.0
35	6288.0
36	7475.5
37	8663.0
38	9933.5
39	11204.0
40	11204.0
41	12647.5
42	14091.0
43	15341.5
44	16592.0
45	18202.5
46	19813.0
47	19813.0
48	21411.0
49	23009.0
50	23964.0
51	24919.0
52	25757.5
53	26596.0
54	26596.0
55	26750.5
56	26905.0
57	26947.5
58	26990.0
59	25978.5
60	24967.0
61	24967.0
62	23101.0
63	21235.0
64	19315.0
65	17395.0
66	15373.0
67	13351.0
68	13351.0
69	11530.5
70	9710.0
71	8380.0
72	7050.0
73	5699.5
74	4349.0
75	4349.0
76	3461.0
77	2573.0
78	2096.0
79	1619.0
80	1325.5
81	1032.0
82	1032.0
83	772.5
84	513.0
85	395.5
86	278.0
87	213.5
88	149.0
89	149.0
90	102.5
91	56.0
92	34.5
93	13.0
94	7.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	321902.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.94972978910616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.21974214945605	73.84213478430406
2	5.487087765296865	8.883565438597833
3	1.4205349209252665	3.4497575401466882
4	0.6150082668241196	1.9913901206991595
5	0.31809436368994665	1.2874826394069423
6	0.18829726246059447	0.9145567509728116
7	0.1478363199502027	0.8377117107089327
8	0.10098878210181872	0.6540011698298517
9	0.06856184326408514	0.49950564174636664
>10	0.40848340702761604	5.9033142640762355
>50	0.02036879859376582	1.1118840386675692
>100	0.0049961204097080675	0.6246959008436082
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT	348	0.10810743642475039	RNA PCR Primer, Index 26 (100% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	3.1065355294468503E-4
17	0.0	0.0	0.0	0.0	3.1065355294468503E-4
18	0.0	0.0	0.0	0.0	3.1065355294468503E-4
19	0.0	0.0	0.0	0.0	3.1065355294468503E-4
20	0.0	0.0	0.0	0.0	3.1065355294468503E-4
21	0.0	0.0	0.0	0.0	3.1065355294468503E-4
22	0.0	0.0	0.0	3.1065355294468503E-4	3.1065355294468503E-4
23	0.0	0.0	0.0	0.0012426142117787401	3.1065355294468503E-4
24	0.0	0.0	0.0	0.00310653552944685	3.1065355294468503E-4
25	0.0	0.0	0.0	0.0043491497412255905	3.1065355294468503E-4
26	0.0	0.0	0.0	0.00745568527067244	3.1065355294468503E-4
27	0.0	0.0	0.0	0.02609489844735354	3.1065355294468503E-4
28	0.0	0.0	0.0	0.09723456207168642	3.1065355294468503E-4
29	0.0	0.0	0.0	0.18173232847264073	3.1065355294468503E-4
30	0.0	0.0	0.0	0.3218370808506937	3.1065355294468503E-4
31	0.0	0.0	0.0	0.7241334319140608	3.1065355294468503E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGATAGT	25	0.0054905396	29.6	6
GGCCATA	25	0.0054905396	29.6	11
TCGTTTA	45	3.9964834E-6	28.777777	30
CGTTTAT	45	3.9964834E-6	28.777777	31
GTCCTAT	60	1.3324152E-6	24.666666	1
TCCTATT	55	1.897147E-5	23.545454	2
ATGGTCG	50	2.6962504E-4	22.2	36
CCTATTC	70	5.083115E-6	21.142859	3
GTACAGA	45	0.0038199173	20.555555	1
TATGGTC	55	5.1320455E-4	20.181818	35
GTTTATG	65	6.882419E-5	19.923077	32
ATCGCCG	65	6.882419E-5	19.923077	16
CTATTCC	75	9.231833E-6	19.733334	4
CTTATAC	285	0.0	18.82456	37
ATTCCAT	70	1.2157401E-4	18.5	6
GGTATCA	210	0.0	18.5	1
TTTATGG	70	1.2157401E-4	18.5	33
GTCTTGG	50	0.007024153	18.5	1
TCCATTA	75	2.0614469E-4	17.266666	8
GCCGCAT	75	2.0614469E-4	17.266666	11
>>END_MODULE