FastQCFastQC Report
Fri 10 Feb 2017
ERR1632109.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632109.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences233478
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG358.848152E-426.4285733
TTAACTG358.848152E-426.428575
CCGTCTT551.8942521E-523.54545437
GTATTAG400.001926605223.1250021
GACCTTT400.001926605223.1250026
GTGTTGA450.003816818820.5555551
CGTATGC450.003816818820.55555531
AGTGACC450.003816818820.5555558
ACTGTCC450.003816818820.5555558
GGTATCA2000.020.351
TATACAC656.8720634E-519.92307737
TTGCCGC500.007018510318.512
TAGGACC609.2067895E-418.54
GCCGTCT609.2067895E-418.536
TGCCGTC650.001574840617.07692335
AGGACCG803.3681438E-416.18755
CACGAGA803.3681438E-416.187511
GTATCAA2550.015.9607842
CACTCTG750.00409139314.7999995
CCACTCT750.00409139314.7999994
Produced by FastQC (version 0.11.5)