##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632109.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233478
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.597011281576854	34.0	31.0	34.0	31.0	34.0
2	32.77308782840353	34.0	31.0	34.0	31.0	34.0
3	32.842816025492766	34.0	31.0	34.0	31.0	34.0
4	36.30193851240802	37.0	37.0	37.0	35.0	37.0
5	36.142711518858306	37.0	35.0	37.0	35.0	37.0
6	36.16165120482444	37.0	36.0	37.0	35.0	37.0
7	36.14002604099744	37.0	35.0	37.0	35.0	37.0
8	36.134385252571974	37.0	35.0	37.0	35.0	37.0
9	37.89264941450586	39.0	38.0	39.0	35.0	39.0
10	37.8254053915144	39.0	37.0	39.0	35.0	39.0
11	37.9083125604982	39.0	38.0	39.0	35.0	39.0
12	37.87052313280052	39.0	38.0	39.0	35.0	39.0
13	37.89745500646742	39.0	38.0	39.0	35.0	39.0
14	39.248768620598085	40.0	39.0	41.0	36.0	41.0
15	39.25340717326686	40.0	39.0	41.0	36.0	41.0
16	39.238013859978246	40.0	39.0	41.0	36.0	41.0
17	39.218307506488834	40.0	39.0	41.0	36.0	41.0
18	39.21091923007735	40.0	39.0	41.0	36.0	41.0
19	39.2403438439596	40.0	39.0	41.0	36.0	41.0
20	39.22884811416922	40.0	39.0	41.0	36.0	41.0
21	39.20167638921012	40.0	39.0	41.0	36.0	41.0
22	39.152185644900165	40.0	39.0	41.0	36.0	41.0
23	39.10624127326772	40.0	39.0	41.0	36.0	41.0
24	39.06884160391986	40.0	39.0	41.0	36.0	41.0
25	39.001700374339336	40.0	38.0	41.0	36.0	41.0
26	38.9183691825354	40.0	38.0	41.0	35.0	41.0
27	38.84074302503876	40.0	38.0	41.0	35.0	41.0
28	38.763982045417556	40.0	38.0	41.0	35.0	41.0
29	38.70895758915187	40.0	38.0	41.0	35.0	41.0
30	38.640587121698836	40.0	38.0	41.0	35.0	41.0
31	38.5694626474443	40.0	38.0	41.0	35.0	41.0
32	38.47356067809387	40.0	38.0	41.0	34.0	41.0
33	38.410162841895165	40.0	38.0	41.0	34.0	41.0
34	38.3322711347536	40.0	38.0	41.0	34.0	41.0
35	38.30503944697145	40.0	38.0	41.0	34.0	41.0
36	38.256949262885584	40.0	38.0	41.0	34.0	41.0
37	38.191448444821354	40.0	38.0	41.0	34.0	41.0
38	38.15546646793274	40.0	38.0	41.0	34.0	41.0
39	38.089610155989	40.0	38.0	41.0	34.0	41.0
40	38.02202777135319	40.0	37.0	41.0	33.0	41.0
41	37.95289920249445	40.0	37.0	41.0	33.0	41.0
42	37.90600399181079	40.0	37.0	41.0	33.0	41.0
43	37.02622088590788	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	13.0
20	11.0
21	39.0
22	73.0
23	99.0
24	187.0
25	253.0
26	412.0
27	582.0
28	849.0
29	1309.0
30	1715.0
31	2471.0
32	3251.0
33	4505.0
34	6621.0
35	9657.0
36	15619.0
37	28723.0
38	68340.0
39	88743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.55304568310505	18.085215737671216	12.559641593640514	26.802096985583223
2	20.639631999588826	19.965906852037453	32.42703809352487	26.96742305484885
3	21.13304037211215	20.927025244348503	28.976605932893033	28.963328450646316
4	16.368994080812755	15.425436229537686	32.32938435312963	35.876185336519924
5	16.966052476036285	33.810466082457445	32.56152613950779	16.661955301998475
6	36.56704271922837	32.34523167064991	14.823666469645962	16.264059140475762
7	30.59860029638767	28.420664902046443	20.279855061290572	20.700879740275315
8	28.137126410197105	31.119848551041212	19.44080384447357	21.302221194288112
9	26.900607337736314	13.801728642527348	19.365422009782506	39.932242009953825
10	18.38759968819332	25.175819563299324	32.01586444975544	24.420716298751916
11	37.33499516014357	20.29099101414266	19.921363040629096	22.45265078508468
12	22.839410993755298	24.339766487634808	27.311780981505752	25.50904153710414
13	32.07111590813695	18.484825122709633	22.82827504090321	26.6157839282502
14	24.43356547511971	20.3595199547709	22.961906475128277	32.24500809498111
15	27.460831427372174	25.83755214624076	20.67989275220792	26.021723674179153
16	26.816659385466725	24.39116319310599	22.52289294922862	26.269284472198667
17	25.33472104438105	24.713249214058713	23.938443879080683	26.013585862479548
18	25.329581373833935	23.231739178851967	24.96380815323071	26.474871294083385
19	26.077403438439596	24.308500158473176	24.321777640719898	25.292318762367334
20	26.701016798156573	23.276282990260324	24.318779499567412	25.703920712015695
21	27.40258182783817	23.525557011795545	23.248871414009027	25.822989746357262
22	26.948577596176087	23.61507294049118	23.565817764414636	25.870531698918096
23	26.264573107530474	23.31782866051619	23.68831324578761	26.72928498616572
24	26.411482024002265	23.86948663257352	23.58680475248203	26.13222659094219
25	26.437608682616776	23.54054771755797	23.615929552249035	26.405914047576218
26	26.393493177087347	23.76326677459975	23.629635340374683	26.21360470793822
27	26.440178517890338	23.164923461739438	23.961144090663787	26.433753929706437
28	25.81827838168907	23.503285106091365	24.13203813635546	26.546398375864104
29	25.234925774591183	23.751274209989806	24.67684321434996	26.33695680106905
30	25.374553491121222	24.30293218204713	24.53507396842529	25.78744035840636
31	26.271426001593294	24.064365807485075	23.515705976580232	26.148502214341395
32	25.392114032157203	23.71615312791783	24.270380935248717	26.621351904676242
33	25.25334292738502	23.534979741131927	24.68926408483883	26.522413246644224
34	26.046565415156884	23.191906732111807	24.46697333367598	26.29455451905533
35	25.68250541806937	23.217605084847396	24.92012095358021	26.17976854350303
36	25.25248631562717	23.770547974541497	24.682839496654932	26.2941262131764
37	25.88166765177019	22.80172007640977	25.100009422729336	26.21660284909071
38	24.788202742870848	22.639820454175556	26.063697650313948	26.508279152639645
39	24.827606883732088	22.32844208019599	26.500141340940047	26.343809695131874
40	24.25667514712307	22.119428811279864	27.1541644180608	26.469731623536262
41	23.578666940782426	22.225220363374707	27.852303000710986	26.343809695131874
42	24.145315618602183	22.248348880836737	27.524649003332218	26.081686497228862
43	23.018442851146574	21.8226128371838	28.105003469277616	27.053940842392006
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	4.0
3	18.0
4	32.0
5	32.0
6	37.0
7	42.0
8	41.5
9	41.0
10	67.5
11	94.0
12	94.0
13	166.0
14	238.0
15	319.5
16	401.0
17	408.5
18	416.0
19	416.0
20	604.0
21	792.0
22	886.5
23	981.0
24	1240.0
25	1499.0
26	1499.0
27	1873.0
28	2247.0
29	2753.0
30	3259.0
31	4030.5
32	4802.0
33	4802.0
34	5646.0
35	6490.0
36	7428.5
37	8367.0
38	9174.5
39	9982.0
40	9982.0
41	10612.5
42	11243.0
43	11986.0
44	12729.0
45	13584.0
46	14439.0
47	14439.0
48	15306.5
49	16174.0
50	16834.0
51	17494.0
52	18434.5
53	19375.0
54	19375.0
55	19000.0
56	18625.0
57	18396.0
58	18167.0
59	17037.0
60	15907.0
61	15907.0
62	14737.0
63	13567.0
64	12213.5
65	10860.0
66	9575.0
67	8290.0
68	8290.0
69	7054.5
70	5819.0
71	5063.0
72	4307.0
73	3483.0
74	2659.0
75	2659.0
76	2104.0
77	1549.0
78	1337.0
79	1125.0
80	906.5
81	688.0
82	688.0
83	533.5
84	379.0
85	290.5
86	202.0
87	158.0
88	114.0
89	114.0
90	84.0
91	54.0
92	34.5
93	15.0
94	9.5
95	4.0
96	4.0
97	3.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	233478.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.00735921341965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.7664780784259	73.29234456506015
2	4.2439172209987674	6.706013847029343
3	0.9506823704288905	2.2533271061502185
4	0.5028371103205879	1.5891132880374643
5	0.2896567045389758	1.144250565204311
6	0.2165061719995563	1.0263348541854818
7	0.1545073529789451	0.8545052556545498
8	0.12300589454030725	0.7774696716251255
9	0.08641105538731711	0.6144398363696817
>10	0.6263795110344744	9.196484231437028
>50	0.032020455225076086	1.6945717890488603
>100	0.007598074121204495	0.8511449901978713
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	4.2830587892649417E-4	0.0
16	0.0	0.0	0.0	4.2830587892649417E-4	0.0
17	0.0	0.0	0.0	0.0012849176367794824	0.0
18	0.0	0.0	0.0	0.0012849176367794824	0.0
19	0.0	0.0	0.0	0.0012849176367794824	0.0
20	0.0	0.0	0.0	0.002141529394632471	0.0
21	0.0	0.0	0.0	0.002141529394632471	0.0
22	0.0	0.0	0.0	0.002569835273558965	0.0
23	0.0	0.0	0.0	0.005996282304970918	0.0
24	0.0	0.0	0.0	0.008137811699603389	0.0
25	0.0	0.0	0.0	0.009851035215309365	0.0
26	0.0	0.0	0.0	0.016703929278133273	0.0
27	0.0	0.0	0.0	0.03811922322445798	0.0
28	0.0	0.0	0.0	0.09636882275846119	0.0
29	0.0	0.0	0.0	0.16189962223421478	0.0
30	0.0	0.0	0.0	0.25998166850838195	0.0
31	0.0	0.0	0.0	0.4899819254919093	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	35	8.848152E-4	26.42857	33
TTAACTG	35	8.848152E-4	26.42857	5
CCGTCTT	55	1.8942521E-5	23.545454	37
GTATTAG	40	0.0019266052	23.125002	1
GACCTTT	40	0.0019266052	23.125002	6
GTGTTGA	45	0.0038168188	20.555555	1
CGTATGC	45	0.0038168188	20.555555	31
AGTGACC	45	0.0038168188	20.555555	8
ACTGTCC	45	0.0038168188	20.555555	8
GGTATCA	200	0.0	20.35	1
TATACAC	65	6.8720634E-5	19.923077	37
TTGCCGC	50	0.0070185103	18.5	12
TAGGACC	60	9.2067895E-4	18.5	4
GCCGTCT	60	9.2067895E-4	18.5	36
TGCCGTC	65	0.0015748406	17.076923	35
AGGACCG	80	3.3681438E-4	16.1875	5
CACGAGA	80	3.3681438E-4	16.1875	11
GTATCAA	255	0.0	15.960784	2
CACTCTG	75	0.004091393	14.799999	5
CCACTCT	75	0.004091393	14.799999	4
>>END_MODULE