FastQCFastQC Report
Fri 10 Feb 2017
ERR1632108.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632108.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8886
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT2052.3069997749268514RNA PCR Primer, Index 17 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1191.3391852352014404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT880.9903218546027459No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC850.9565608822867432No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT720.8102633355840648No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC630.7089804186360568No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT540.6076975016880486No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC520.5851901868107134RNA PCR Primer, Index 17 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG470.5289218996173757No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.337609723160027No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC200.22507314877335136No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG200.22507314877335136No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA190.21381949133468378No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA180.2025658338960162No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC160.18005851901868106No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA160.18005851901868106No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA160.18005851901868106No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT150.1688048615800135No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA130.14629754670267836No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1350438892640108No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG120.1350438892640108No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA120.1350438892640108No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT120.1350438892640108No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1350438892640108No Hit
GGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT110.12379023182534324No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT110.12379023182534324No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC110.12379023182534324No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.11253657438667568No Hit
GTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAA100.11253657438667568No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT100.11253657438667568No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG100.11253657438667568No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC100.11253657438667568No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT100.11253657438667568No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC100.11253657438667568No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG90.1012829169480081No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.1012829169480081No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTA90.1012829169480081No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT90.1012829169480081No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGA90.1012829169480081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA909.458745E-1124.6666661
AACGCAG951.8553692E-1023.3684227
CAACGCA1003.5470293E-1022.26
TCAACGC1056.511982E-1021.1428575
AGTACAT704.226067E-621.14285714
GAGTACA704.226067E-621.14285713
ATCAACG1056.511982E-1021.1428574
GTATCAA1101.1659722E-920.1818182
ACGCAGA1152.0299922E-919.3043488
CGCAGAG1152.0299922E-919.3043489
TATCAAC1152.0299922E-919.3043483
CAGAGTA1203.448804E-918.511
AGAGTAC1203.448804E-918.512
GCAGAGT1203.448804E-918.510
ACATGGG852.2635555E-517.41176417
GTACATG852.2635555E-517.41176415
TACATGG852.2635555E-517.41176416
CTGTCTC751.7908853E-417.26666636
TGTCTCT802.9333244E-416.187537
Produced by FastQC (version 0.11.5)