##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632108.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8886
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.736776952509565	34.0	31.0	34.0	31.0	34.0
2	32.87846049966239	34.0	31.0	34.0	31.0	34.0
3	32.89196488858879	34.0	31.0	34.0	31.0	34.0
4	36.32421787080801	37.0	37.0	37.0	35.0	37.0
5	36.234638757596215	37.0	35.0	37.0	35.0	37.0
6	36.310150799009676	37.0	37.0	37.0	35.0	37.0
7	36.24904343911771	37.0	36.0	37.0	35.0	37.0
8	36.30328606797209	37.0	37.0	37.0	35.0	37.0
9	38.069322529822195	39.0	38.0	39.0	37.0	39.0
10	38.080351114112084	39.0	38.0	39.0	35.0	39.0
11	38.09858203916273	39.0	38.0	39.0	37.0	39.0
12	38.06493360342111	39.0	38.0	39.0	37.0	39.0
13	38.131892865181186	39.0	39.0	39.0	37.0	39.0
14	39.421336934503714	40.0	39.0	41.0	37.0	41.0
15	39.461625028134144	40.0	39.0	41.0	37.0	41.0
16	39.38543776727436	40.0	39.0	41.0	36.0	41.0
17	39.344136844474455	40.0	39.0	41.0	37.0	41.0
18	39.442718883637184	40.0	39.0	41.0	37.0	41.0
19	39.489309025433265	40.0	39.0	41.0	37.0	41.0
20	39.527233851001576	40.0	39.0	41.0	37.0	41.0
21	39.516092730137295	41.0	39.0	41.0	37.0	41.0
22	39.43225298221922	41.0	39.0	41.0	37.0	41.0
23	39.317353139770425	40.0	39.0	41.0	36.0	41.0
24	39.28910645959937	40.0	39.0	41.0	36.0	41.0
25	39.17071798334459	40.0	39.0	41.0	35.0	41.0
26	39.09835696601395	40.0	38.0	41.0	35.0	41.0
27	38.978843124015306	40.0	38.0	41.0	35.0	41.0
28	38.883749718658564	40.0	38.0	41.0	35.0	41.0
29	38.805649336034215	40.0	38.0	41.0	35.0	41.0
30	38.69671393202791	40.0	38.0	41.0	35.0	41.0
31	38.49876209768175	40.0	38.0	41.0	35.0	41.0
32	38.301710555930676	40.0	37.0	41.0	35.0	41.0
33	38.16993022732388	40.0	37.0	41.0	35.0	41.0
34	38.007990096781455	40.0	37.0	41.0	34.0	41.0
35	37.93056493360342	40.0	37.0	41.0	34.0	41.0
36	37.822192212469055	40.0	37.0	41.0	34.0	41.0
37	37.68253432365519	40.0	36.0	41.0	33.0	41.0
38	37.57449921224398	40.0	36.0	41.0	33.0	41.0
39	37.35212694125591	40.0	36.0	41.0	33.0	41.0
40	37.17837047040288	40.0	35.0	41.0	33.0	41.0
41	36.99572361017331	40.0	35.0	41.0	32.0	41.0
42	36.82286743191537	40.0	35.0	41.0	32.0	41.0
43	35.91154625253207	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
23	3.0
24	6.0
25	8.0
26	15.0
27	27.0
28	47.0
29	58.0
30	84.0
31	111.0
32	150.0
33	190.0
34	243.0
35	370.0
36	591.0
37	1176.0
38	2281.0
39	3526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.88453747467927	17.735764123340086	15.923925275714607	25.455773126266035
2	19.029934728786856	20.4704028809363	33.49088453747468	27.00877785280216
3	20.414134593742965	22.270988071123117	32.55683097006527	24.758046365068648
4	14.483457123565158	18.45599819941481	29.529597119063695	37.53094755795633
5	14.01080351114112	35.921674544226875	32.24172856178258	17.825793382849426
6	30.80126040963313	30.947557956335807	18.08462750393878	20.16655413009228
7	26.27729011928877	32.60184559981994	19.446320054017555	21.674544226873735
8	32.34301147873059	29.10195813639433	18.804861580013505	19.75016880486158
9	25.782129191987398	13.436866981769075	18.95115912671618	41.82984469952735
10	20.830519918973668	24.454197614224622	26.69367544451947	28.02160702228224
11	31.510240828269186	23.002475804636507	18.163403106009454	27.323880261084852
12	21.246905244204367	29.88971415710106	24.61174881836597	24.251631780328605
13	33.91852352014404	17.274364168354715	23.857753769975243	24.949358541525996
14	24.79180733738465	23.40760747242854	23.238802610848523	28.561782579338285
15	31.92662615349989	24.65676344812064	20.132793157776277	23.283817240603195
16	27.94283142021157	23.621426963763223	24.89309025433266	23.542651361692553
17	20.346612649110963	31.577762772901192	24.40918298446995	23.66644159351789
18	22.38352464550979	21.92212469052442	26.81746567634481	28.876884987620976
19	23.238802610848523	30.159801935629076	26.952509565608825	19.648885887913572
20	26.558631555255456	21.246905244204367	28.78685572811164	23.40760747242854
21	30.092279990997074	21.111861354940356	25.669592617600724	23.12626603646185
22	26.73869007427414	23.160027008777853	24.54422687373396	25.557056043214043
23	25.602070672968715	24.701778077875307	24.55548053117263	25.140670717983344
24	24.904343911771328	25.174431690299347	26.81746567634481	23.103758721584512
25	22.75489534098582	27.50393878010353	25.016880486158	24.724285392752645
26	25.050641458474004	25.38825118163403	25.62457798784605	23.936529372045914
27	23.160027008777853	25.93968039612874	25.725860904794057	25.174431690299347
28	21.92212469052442	24.431690299347288	28.831870357866308	24.814314652261984
29	20.988071123115013	25.106909745667345	28.39297771775827	25.512041413459375
30	22.11343686698177	25.883412108935406	30.497411658789108	21.50573936529372
31	22.214719783929777	25.48953409858204	26.232275489534096	26.063470627954082
32	23.40760747242854	25.25320729237002	29.32703128516768	22.01215395003376
33	21.190636957011026	25.106909745667345	30.587440918298448	23.115012379023185
34	23.790231825343238	26.22102183209543	26.69367544451947	23.295070898041864
35	21.14562232725636	25.917173081251406	30.756245779878462	22.180958811613774
36	22.57483682196714	25.75962187711006	27.368894890839524	24.296646410083277
37	20.71798334458699	25.331982894440692	28.550528921899616	25.3995048390727
38	21.359441818591044	22.642358766599145	30.373621426963766	25.62457798784605
39	23.722709880711232	22.38352464550979	29.788431240153052	24.10533423362593
40	20.312851676794956	21.888363718208417	32.230474904343914	25.568309700652712
41	20.189061444969614	22.66486608147648	30.114787305874408	27.0312851676795
42	18.613549403556156	22.451046590141797	34.15485032635607	24.78055367994598
43	18.242178708080125	23.475129417060543	33.513391852352015	24.769300022507316
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	6.0
4	12.0
5	12.0
6	21.0
7	30.0
8	23.5
9	17.0
10	30.5
11	44.0
12	44.0
13	68.0
14	92.0
15	136.0
16	180.0
17	161.0
18	142.0
19	142.0
20	181.5
21	221.0
22	147.5
23	74.0
24	64.5
25	55.0
26	55.0
27	59.0
28	63.0
29	86.0
30	109.0
31	112.5
32	116.0
33	116.0
34	117.5
35	119.0
36	133.0
37	147.0
38	184.5
39	222.0
40	222.0
41	261.5
42	301.0
43	365.5
44	430.0
45	545.0
46	660.0
47	660.0
48	838.0
49	1016.0
50	1038.5
51	1061.0
52	937.0
53	813.0
54	813.0
55	822.0
56	831.0
57	749.5
58	668.0
59	510.0
60	352.0
61	352.0
62	315.0
63	278.0
64	259.0
65	240.0
66	218.0
67	196.0
68	196.0
69	170.5
70	145.0
71	113.5
72	82.0
73	73.5
74	65.0
75	65.0
76	64.0
77	63.0
78	41.5
79	20.0
80	17.0
81	14.0
82	14.0
83	10.0
84	6.0
85	3.5
86	1.0
87	1.0
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	8886.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.63583164528471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.685909834846	68.59104208867882
2	5.728314238952537	8.665316227774026
3	1.3688439220354114	3.1060094530722484
4	0.7290581758666865	2.2057168579788433
5	0.34221098050885285	1.29417060544677
6	0.2529385508108912	1.1478730587440917
7	0.17854485939592324	0.9453072248480756
8	0.1339086445469424	0.8102633355840648
9	0.074393691414968	0.5064145847400405
>10	0.3868471953578337	4.422687373396354
>50	0.08927242969796162	4.659014179608373
>100	0.029757476565987203	3.6461850101282915
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	205	2.3069997749268514	RNA PCR Primer, Index 17 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	119	1.3391852352014404	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	88	0.9903218546027459	No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	85	0.9565608822867432	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	72	0.8102633355840648	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	63	0.7089804186360568	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	54	0.6076975016880486	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	52	0.5851901868107134	RNA PCR Primer, Index 17 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	47	0.5289218996173757	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30	0.337609723160027	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	20	0.22507314877335136	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	20	0.22507314877335136	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	19	0.21381949133468378	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	18	0.2025658338960162	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	16	0.18005851901868106	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	16	0.18005851901868106	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	16	0.18005851901868106	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	15	0.1688048615800135	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	13	0.14629754670267836	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.1350438892640108	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	12	0.1350438892640108	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	12	0.1350438892640108	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGACTGTCTCT	12	0.1350438892640108	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12	0.1350438892640108	No Hit
GGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCCACACT	11	0.12379023182534324	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	11	0.12379023182534324	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	11	0.12379023182534324	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10	0.11253657438667568	No Hit
GTTTACTGGGACTTCAATCAAGAGCTTGCACCCCATCATTTAA	10	0.11253657438667568	No Hit
GTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAAT	10	0.11253657438667568	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	10	0.11253657438667568	No Hit
TTCCTCGCCTTATTGATATGCCCATAGAGAGGCCCATGTACTC	10	0.11253657438667568	No Hit
GTCGGTGATTCCTCGCCTTATTGATATGCCCATAGAGCTGTCT	10	0.11253657438667568	No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC	10	0.11253657438667568	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG	9	0.1012829169480081	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9	0.1012829169480081	No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTA	9	0.1012829169480081	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	9	0.1012829169480081	No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGA	9	0.1012829169480081	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.022507314877335136	0.0
10	0.0	0.0	0.0	0.022507314877335136	0.0
11	0.0	0.0	0.0	0.022507314877335136	0.0
12	0.0	0.0	0.0	0.022507314877335136	0.0
13	0.0	0.0	0.0	0.022507314877335136	0.0
14	0.0	0.0	0.0	0.022507314877335136	0.0
15	0.0	0.0	0.0	0.022507314877335136	0.0
16	0.0	0.0	0.0	0.022507314877335136	0.0
17	0.0	0.0	0.0	0.022507314877335136	0.0
18	0.0	0.0	0.0	0.022507314877335136	0.0
19	0.0	0.0	0.0	0.022507314877335136	0.0
20	0.0	0.0	0.0	0.022507314877335136	0.0
21	0.0	0.0	0.0	0.022507314877335136	0.0
22	0.0	0.0	0.0	0.022507314877335136	0.0
23	0.0	0.0	0.0	0.022507314877335136	0.0
24	0.0	0.0	0.0	0.05626828719333783	0.0
25	0.0	0.0	0.0	0.05626828719333783	0.0
26	0.0	0.0	0.0	0.0675219446320054	0.0
27	0.0	0.0	0.0	0.09002925950934054	0.0
28	0.0	0.0	0.0	0.1688048615800135	0.0
29	0.0	0.0	0.0	0.2363268062120189	0.0
30	0.0	0.0	0.0	0.4839072698627054	0.0
31	0.0	0.0	0.0	0.9565608822867432	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	90	9.458745E-11	24.666666	1
AACGCAG	95	1.8553692E-10	23.368422	7
CAACGCA	100	3.5470293E-10	22.2	6
TCAACGC	105	6.511982E-10	21.142857	5
AGTACAT	70	4.226067E-6	21.142857	14
GAGTACA	70	4.226067E-6	21.142857	13
ATCAACG	105	6.511982E-10	21.142857	4
GTATCAA	110	1.1659722E-9	20.181818	2
ACGCAGA	115	2.0299922E-9	19.304348	8
CGCAGAG	115	2.0299922E-9	19.304348	9
TATCAAC	115	2.0299922E-9	19.304348	3
CAGAGTA	120	3.448804E-9	18.5	11
AGAGTAC	120	3.448804E-9	18.5	12
GCAGAGT	120	3.448804E-9	18.5	10
ACATGGG	85	2.2635555E-5	17.411764	17
GTACATG	85	2.2635555E-5	17.411764	15
TACATGG	85	2.2635555E-5	17.411764	16
CTGTCTC	75	1.7908853E-4	17.266666	36
TGTCTCT	80	2.9333244E-4	16.1875	37
>>END_MODULE