FastQCFastQC Report
Fri 10 Feb 2017
ERR1632107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457118
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACA451.3215093E-424.6666664
ATTGAAC400.001929417623.1253
GGTATCA2350.022.0425551
TCTAGAC1201.0913936E-1121.5833323
CGATGCG450.00382233620.55555533
TATACTG450.00382233620.5555555
CTAGACA1254.0927262E-1019.244
CTTATAC3150.018.7936537
TAGAAGT801.6132015E-518.54
TTATACT500.007028558818.54
CCGACCT500.007028558818.536
CTCTAGT1205.149559E-918.527
TCTTATA5900.017.8728837
GTTCTAG1451.546141E-1017.8620681
CGGGAGT1258.538336E-917.764
GTGCATT852.7173104E-517.41176411
CGGTCCA852.7173104E-517.41176410
AGACATC752.0638356E-417.2666666
AACCTCG650.001577966117.0769239
AATCGAA1201.0371332E-716.9583327