##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632107.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	457118
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86286691838869	34.0	31.0	34.0	31.0	34.0
2	33.027443679750085	34.0	33.0	34.0	31.0	34.0
3	33.10767460480664	34.0	33.0	34.0	31.0	34.0
4	36.46834734138669	37.0	37.0	37.0	35.0	37.0
5	36.34666322481285	37.0	37.0	37.0	35.0	37.0
6	36.37166552181275	37.0	37.0	37.0	35.0	37.0
7	36.35559308537402	37.0	37.0	37.0	35.0	37.0
8	36.339767849876836	37.0	37.0	37.0	35.0	37.0
9	38.14676298023705	39.0	39.0	39.0	37.0	39.0
10	38.1170266758255	39.0	38.0	39.0	37.0	39.0
11	38.1852147585525	39.0	39.0	39.0	37.0	39.0
12	38.15016472770707	39.0	38.0	39.0	37.0	39.0
13	38.17359850191854	39.0	39.0	39.0	37.0	39.0
14	39.584724294383506	41.0	39.0	41.0	37.0	41.0
15	39.60684768484286	41.0	39.0	41.0	37.0	41.0
16	39.597543741440944	41.0	39.0	41.0	37.0	41.0
17	39.59118652076707	41.0	39.0	41.0	37.0	41.0
18	39.585997488613444	41.0	39.0	41.0	37.0	41.0
19	39.60282027835264	41.0	39.0	41.0	37.0	41.0
20	39.58477679723835	41.0	39.0	41.0	37.0	41.0
21	39.560796118288934	41.0	39.0	41.0	37.0	41.0
22	39.52111927336049	41.0	39.0	41.0	37.0	41.0
23	39.48160868747238	41.0	39.0	41.0	37.0	41.0
24	39.42756356126864	41.0	39.0	41.0	37.0	41.0
25	39.37334780078667	40.0	39.0	41.0	37.0	41.0
26	39.30953714358218	40.0	39.0	41.0	36.0	41.0
27	39.229977817543826	40.0	39.0	41.0	36.0	41.0
28	39.16291417095805	40.0	39.0	41.0	36.0	41.0
29	39.115252516855605	40.0	39.0	41.0	36.0	41.0
30	39.057575943191914	40.0	39.0	41.0	35.0	41.0
31	38.99517411259237	40.0	39.0	41.0	35.0	41.0
32	38.911407995309744	40.0	38.0	41.0	35.0	41.0
33	38.83951408607843	40.0	38.0	41.0	35.0	41.0
34	38.76904869202263	40.0	38.0	41.0	35.0	41.0
35	38.75163962040436	40.0	38.0	41.0	35.0	41.0
36	38.718328746625595	40.0	38.0	41.0	35.0	41.0
37	38.65897208160694	40.0	38.0	41.0	35.0	41.0
38	38.623007626039666	40.0	38.0	41.0	35.0	41.0
39	38.57267707681605	40.0	38.0	41.0	35.0	41.0
40	38.50639441019605	40.0	38.0	41.0	35.0	41.0
41	38.456577951426105	40.0	38.0	41.0	34.0	41.0
42	38.397520990203844	40.0	38.0	41.0	34.0	41.0
43	37.518918528694996	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	1.0
17	2.0
18	4.0
19	7.0
20	30.0
21	37.0
22	66.0
23	126.0
24	240.0
25	358.0
26	569.0
27	790.0
28	1158.0
29	1616.0
30	2396.0
31	3323.0
32	4825.0
33	6598.0
34	9706.0
35	15211.0
36	24676.0
37	46010.0
38	113711.0
39	225655.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.975118022042444	17.70133751022712	12.033435568059012	27.290108899671417
2	20.678905665495563	19.51662371641458	32.28575553795738	27.518715080132484
3	21.184683167147213	19.977336267659553	28.29685114128081	30.541129423912427
4	16.159066149221864	14.70101811786016	31.782603179047857	37.357312553870116
5	17.597863133807902	33.325968349529006	31.879952222402093	17.196216294261
6	37.54894797404609	32.054305452859	14.487944031956737	15.908802541138176
7	31.831387081672567	27.81776259084088	19.51793628778565	20.83291403970091
8	28.38741856588452	30.968808928985514	19.172948779089865	21.470823726040102
9	27.51083965190607	13.442262173005656	18.653389278041992	40.393508897046274
10	18.663014801429828	25.053924807161387	31.127630064884777	25.155430326524
11	38.53643916887981	19.903175985194192	19.571314190209094	21.989070655716905
12	23.992710853652667	23.539654968738922	26.770768160518728	25.696866017089683
13	32.569270954108134	17.730432842285797	22.528099965435622	27.17219623817045
14	24.614650921643864	19.24842163292629	22.644262531775166	33.49266491365468
15	27.933487633390065	25.22871556140865	20.051496550124913	26.786300255076366
16	27.227542997650495	23.98374161595037	21.879033422442344	26.90968196395679
17	26.113388665508687	24.04499494660022	23.23645098202215	26.605165405868945
18	26.34965151230098	22.79980223924676	24.013274471799402	26.837271776652855
19	26.722859305474735	23.292454027187727	23.537248587891966	26.44743807944557
20	27.171977476275273	22.892994806592608	23.447774972764144	26.48725274436797
21	27.625908408769728	22.95118547070997	23.187667079397443	26.23523904112286
22	27.798949067855567	22.966061279582078	22.581259105963884	26.65373054659847
23	26.731828543177034	23.041534133418505	23.160540604395365	27.066096719009096
24	26.763330256082675	23.24738907678105	22.911589567682743	27.07769109945353
25	26.97465424682467	22.749487003355807	23.16863479451695	27.10722395530257
26	27.15228890570925	23.073035846324146	22.9209963291754	26.853678918791207
27	26.62244759558801	23.04109660962815	23.332049930214954	27.004405864568888
28	26.247270945357652	22.85908671284001	23.777230386902286	27.116411954900048
29	25.9727247669092	23.312361359648932	23.721446103631884	26.993467769809982
30	25.721148587454444	23.55693715845799	23.92380085667158	26.798113397415985
31	27.007906054891734	23.15113384290271	22.970655279380818	26.870304822824743
32	25.950848577391394	23.334237549166737	23.26642136166154	27.448492511780326
33	25.94144181589874	22.833710332999356	23.985054187321435	27.23979366378047
34	26.76245520850196	22.4675029204713	23.847234193359267	26.92280767766747
35	26.01691466973517	22.834147856789713	24.510301497643937	26.63863597583119
36	25.920878197752	22.295774832756532	24.627776635354547	27.155570334136918
37	25.700147445517352	21.91775427788886	25.088270424704344	27.293827851889446
38	25.106427662004123	21.469948678459392	26.229113708057877	27.19450995147861
39	25.08148880595382	21.297564305059087	26.671231498212716	26.949715390774376
40	24.446423024251942	21.48548077301703	27.233230806925125	26.834865395805895
41	24.38516969360209	21.074864695767833	27.819293924107125	26.720671686522955
42	23.97958513994198	21.378287444379787	28.040243438236956	26.601883977441272
43	22.799364715456402	20.940763654023687	28.406013326974655	27.853858303545252
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	8.5
2	10.0
3	24.5
4	39.0
5	39.0
6	50.5
7	62.0
8	58.5
9	55.0
10	85.5
11	116.0
12	116.0
13	204.5
14	293.0
15	406.5
16	520.0
17	549.0
18	578.0
19	578.0
20	732.5
21	887.0
22	992.5
23	1098.0
24	1411.5
25	1725.0
26	1725.0
27	2199.0
28	2673.0
29	3422.5
30	4172.0
31	5202.0
32	6232.0
33	6232.0
34	7826.0
35	9420.0
36	11305.0
37	13190.0
38	15101.5
39	17013.0
40	17013.0
41	19008.0
42	21003.0
43	23225.5
44	25448.0
45	28129.5
46	30811.0
47	30811.0
48	32389.0
49	33967.0
50	35378.5
51	36790.0
52	37353.5
53	37917.0
54	37917.0
55	37423.5
56	36930.0
57	36846.0
58	36762.0
59	35663.0
60	34564.0
61	34564.0
62	32015.0
63	29466.0
64	26681.0
65	23896.0
66	21013.5
67	18131.0
68	18131.0
69	15209.0
70	12287.0
71	10499.5
72	8712.0
73	6764.0
74	4816.0
75	4816.0
76	3841.0
77	2866.0
78	2366.0
79	1866.0
80	1598.0
81	1330.0
82	1330.0
83	1009.5
84	689.0
85	564.0
86	439.0
87	325.0
88	211.0
89	211.0
90	161.0
91	111.0
92	62.5
93	14.0
94	8.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	457118.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.02230650731201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.21447086561001	59.32529469093558
2	6.900106139944493	9.387222695685796
3	2.162604601627534	4.413160591980944
4	1.1242482134872849	3.0589582627252017
5	0.6269300283086847	2.132261327212557
6	0.39075130568058375	1.5947883049882223
7	0.2788389329780952	1.3277087155645473
8	0.1807976918914716	0.9838620810924992
9	0.14193079349299734	0.8689013944026002
>10	0.8924802932290069	11.60279785564287
>50	0.06593493500892889	3.1316562662495175
>100	0.020906198740880306	2.1733878135197195
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.187618951780503E-4	0.0
13	0.0	0.0	0.0	2.187618951780503E-4	0.0
14	0.0	0.0	0.0	2.187618951780503E-4	0.0
15	0.0	0.0	0.0	4.375237903561006E-4	0.0
16	0.0	0.0	0.0	0.0010938094758902515	0.0
17	0.0	0.0	0.0	0.0026251427421366035	0.0
18	0.0	0.0	0.0	0.002843904637314654	0.0
19	0.0	0.0	0.0	0.003500190322848805	0.0
20	0.0	0.0	0.0	0.003718952218026855	0.0
21	0.0	0.0	0.0	0.004156476008382956	0.0
22	0.0	0.0	0.0	0.005250285484273207	0.0
23	0.0	0.0	0.0	0.00809419012158786	0.0
24	0.0	0.0	0.0	0.011156856654080565	0.0
25	0.0	0.0	0.0	0.01334447560586107	0.0
26	0.0	0.0	0.0	0.018375999194956225	0.0
27	0.0	0.0	0.0	0.03631447459955635	0.0
28	0.0	0.0	0.0	0.11769389960579106	0.0
29	0.0	0.0	0.0	0.22313713308161132	0.0
30	0.0	0.0	0.0	0.3482689371234561	0.0
31	0.0	0.0	0.0	0.6982879694083366	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACACA	45	1.3215093E-4	24.666666	4
ATTGAAC	40	0.0019294176	23.125	3
GGTATCA	235	0.0	22.042555	1
TCTAGAC	120	1.0913936E-11	21.583332	3
CGATGCG	45	0.003822336	20.555555	33
TATACTG	45	0.003822336	20.555555	5
CTAGACA	125	4.0927262E-10	19.24	4
CTTATAC	315	0.0	18.79365	37
TAGAAGT	80	1.6132015E-5	18.5	4
TTATACT	50	0.0070285588	18.5	4
CCGACCT	50	0.0070285588	18.5	36
CTCTAGT	120	5.149559E-9	18.5	27
TCTTATA	590	0.0	17.87288	37
GTTCTAG	145	1.546141E-10	17.862068	1
CGGGAGT	125	8.538336E-9	17.76	4
GTGCATT	85	2.7173104E-5	17.411764	11
CGGTCCA	85	2.7173104E-5	17.411764	10
AGACATC	75	2.0638356E-4	17.266666	6
AACCTCG	65	0.0015779661	17.076923	9
AATCGAA	120	1.0371332E-7	16.958332	7
>>END_MODULE