FastQCFastQC Report
Fri 10 Feb 2017
ERR1632106.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632106.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455322
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23980.526660253622711No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19840.4357355893192071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16330.35864728697493203No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8120.17833533191894965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTC358.8611373E-426.428575
TATAATA706.5701897E-926.428572
CCTAGAC509.075355E-625.8999983
GGTATCA8150.023.607361
TATACGT551.8993887E-523.5454544
CGTGTTT400.001929405723.12500218
TATAATC802.7166607E-823.1250023
AACCGCG603.7199265E-521.5833347
GTCTCGC450.003822313420.5555551
ATATAAT450.003822313420.5555551
CGTCCAC450.003822313420.5555558
CTAGCGG1052.2504537E-819.3809529
TCTAGCG1103.8364306E-818.528
TAATACC500.00702851918.4999984
CTATACG500.00702851918.4999983
TCGTGTT500.00702851918.49999817
CGGTCCA1258.538336E-917.76000210
AGGCCCG953.5967169E-617.52631610
TTTCGGA752.0638134E-417.26666630
TAGAACC650.00157795417.0769234