##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632106.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455322
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.197769929851844	33.0	31.0	34.0	30.0	34.0
2	32.37811922112263	34.0	31.0	34.0	31.0	34.0
3	32.457401575149014	34.0	31.0	34.0	31.0	34.0
4	36.05267920284985	37.0	35.0	37.0	35.0	37.0
5	35.755889238824395	37.0	35.0	37.0	35.0	37.0
6	35.774678139865856	37.0	35.0	37.0	35.0	37.0
7	35.74678798740232	37.0	35.0	37.0	35.0	37.0
8	35.73035126789393	37.0	35.0	37.0	33.0	37.0
9	37.42984964486671	39.0	37.0	39.0	35.0	39.0
10	37.30251777862699	39.0	37.0	39.0	34.0	39.0
11	37.410237150851486	39.0	37.0	39.0	35.0	39.0
12	37.36546663679769	39.0	37.0	39.0	35.0	39.0
13	37.402438274451924	39.0	37.0	39.0	35.0	39.0
14	38.60116357215333	40.0	38.0	41.0	35.0	41.0
15	38.58790701964763	40.0	38.0	41.0	35.0	41.0
16	38.57629765308946	40.0	38.0	41.0	35.0	41.0
17	38.57331295215254	40.0	38.0	41.0	35.0	41.0
18	38.571591093775396	40.0	38.0	41.0	35.0	41.0
19	38.62697168157919	40.0	38.0	41.0	35.0	41.0
20	38.62179732145602	40.0	38.0	41.0	35.0	41.0
21	38.5877510860446	40.0	38.0	41.0	35.0	41.0
22	38.53344665972652	40.0	38.0	41.0	34.0	41.0
23	38.46274943885865	40.0	38.0	41.0	34.0	41.0
24	38.406707780427915	40.0	38.0	41.0	34.0	41.0
25	38.33985618968554	40.0	38.0	41.0	34.0	41.0
26	38.23135495319796	40.0	38.0	41.0	34.0	41.0
27	38.12968624402072	40.0	38.0	41.0	34.0	41.0
28	38.04183413057133	40.0	38.0	41.0	34.0	41.0
29	37.94311058986827	40.0	37.0	41.0	33.0	41.0
30	37.83747545692938	40.0	37.0	41.0	33.0	41.0
31	37.74494094289316	40.0	37.0	41.0	33.0	41.0
32	37.61123117266462	39.0	37.0	41.0	33.0	41.0
33	37.50611215798929	39.0	37.0	41.0	33.0	41.0
34	37.42254492425141	39.0	36.0	40.0	33.0	41.0
35	37.35295680858821	39.0	36.0	40.0	32.0	41.0
36	37.297003000074675	39.0	36.0	40.0	32.0	41.0
37	37.21146133944769	39.0	36.0	40.0	32.0	41.0
38	37.14376638950018	39.0	36.0	40.0	32.0	41.0
39	37.07348425949109	39.0	36.0	40.0	31.0	41.0
40	36.96347420067556	39.0	36.0	40.0	31.0	41.0
41	36.89224548780863	39.0	36.0	40.0	31.0	41.0
42	36.803343567848685	39.0	35.0	40.0	31.0	41.0
43	35.90146094412306	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	2.0
15	3.0
16	6.0
17	8.0
18	20.0
19	30.0
20	72.0
21	115.0
22	214.0
23	354.0
24	636.0
25	924.0
26	1447.0
27	2067.0
28	2879.0
29	4147.0
30	5652.0
31	7481.0
32	9728.0
33	13416.0
34	18764.0
35	27510.0
36	44416.0
37	79483.0
38	139336.0
39	96610.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.660626984859064	20.304312113185834	13.260505751973328	24.774555149981772
2	19.109772864039076	22.06943657455603	33.957506995049656	24.86328356635524
3	19.78402097856023	23.405194565604123	31.068782092672876	25.742002363162776
4	14.398601429318154	16.933510790166082	35.649276775556636	33.018611004959126
5	14.672473546193682	36.156610047395034	34.11519759642626	15.055718809985022
6	32.657547845261156	36.08127874339478	16.52654604873035	14.734627362613711
7	27.63187370695903	31.282037766679405	21.922946837622607	19.163141688738957
8	26.85681781244921	32.45812853321386	21.100232363030997	19.584821291305932
9	26.191135064855203	14.147570290915002	21.34094113616298	38.320353508066816
10	16.846539372136643	26.76479502418069	33.48948656115891	22.899179042523752
11	34.704011666469	22.52647576879659	21.688167933901724	21.081344630832685
12	21.399800580687952	26.644221012821696	29.27159241152415	22.6843859949662
13	30.1573392017078	20.103575052380513	25.05677300899144	24.682312736920245
14	22.292136114661712	22.31980883858017	25.826557908469173	29.561497138288946
15	25.286280917680237	28.322374056162452	22.94486099946851	23.446484026688804
16	24.063190445443006	26.96882645688106	24.93641862242545	24.031564475250484
17	22.35604692942577	27.0920359657561	26.627309903760416	23.92460720105771
18	22.606858443035918	25.1329828121637	28.42493883449515	23.835219910305234
19	22.866235323573207	26.277008358919623	28.05794580538608	22.798810512121094
20	24.008723496778103	25.377425206776742	27.59321974339039	23.02063155305476
21	24.820895981305537	25.682923293844794	26.634118272343528	22.862062452506137
22	24.421618107624933	25.60583499150052	26.819042348052584	23.15350455282196
23	23.38389096068277	25.272224930928	27.189549373849715	24.15433473453951
24	23.144060686722803	26.098673027000675	27.280913287739228	23.476352998537298
25	23.25936370304971	25.761329344947093	27.08215285007094	23.89715410193226
26	23.287256051761172	26.098892651793676	27.06524174100966	23.548609555435494
27	23.245746965883484	25.700493277285087	27.182960630059604	23.87079912677182
28	22.763890170033516	25.827656032434188	27.753106592697037	23.65534720483526
29	22.28664549483662	26.09691602865664	28.20311779356148	23.41332068294526
30	22.458611707758465	26.726799934991064	27.949670782435287	22.864917574815184
31	23.28000843359205	26.601833427771993	27.008358919621717	23.109799219014235
32	22.268197012224316	26.305120332424085	27.32637561989098	24.10030703546062
33	22.058674959698852	25.94559454627714	28.106702509432886	23.889027984591124
34	22.828679483969587	25.520181322229103	27.974927633630703	23.676211560170604
35	22.725895080843888	25.874655738136966	28.26241648767246	23.137032693346686
36	22.408976504539645	26.272396238266545	27.67667716473177	23.64195009246204
37	22.691413988342315	25.528307439570234	28.072660666517322	23.707617905570125
38	22.210435691664358	25.02229191648987	29.035935008631252	23.731337383214516
39	22.14762300086532	24.596000193269816	29.34802183948942	23.908354966375445
40	21.39716508317191	24.58941144947971	30.193138042967394	23.820285424380987
41	21.02665805737478	24.534724876021805	30.49534175814039	23.943275308463022
42	21.27505369826189	24.343431681315643	30.656765980998063	23.724748639424405
43	20.490553937652916	23.73990275014166	30.898792502888067	24.870750809317364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	36.0
2	61.0
3	177.5
4	294.0
5	294.0
6	395.0
7	496.0
8	492.0
9	488.0
10	734.5
11	981.0
12	981.0
13	1704.0
14	2427.0
15	3648.5
16	4870.0
17	4582.0
18	4294.0
19	4294.0
20	4943.5
21	5593.0
22	5216.0
23	4839.0
24	5668.5
25	6498.0
26	6498.0
27	7640.5
28	8783.0
29	10381.0
30	11979.0
31	13759.5
32	15540.0
33	15540.0
34	17580.5
35	19621.0
36	21563.0
37	23505.0
38	24770.0
39	26035.0
40	26035.0
41	27060.5
42	28086.0
43	28423.0
44	28760.0
45	29507.0
46	30254.0
47	30254.0
48	31016.5
49	31779.0
50	31737.5
51	31696.0
52	31579.5
53	31463.0
54	31463.0
55	30317.0
56	29171.0
57	28242.5
58	27314.0
59	24980.0
60	22646.0
61	22646.0
62	20247.0
63	17848.0
64	15505.0
65	13162.0
66	11277.5
67	9393.0
68	9393.0
69	7788.0
70	6183.0
71	5396.5
72	4610.0
73	3505.0
74	2400.0
75	2400.0
76	1873.5
77	1347.0
78	1127.5
79	908.0
80	796.0
81	684.0
82	684.0
83	585.0
84	486.0
85	420.5
86	355.0
87	311.0
88	267.0
89	267.0
90	214.5
91	162.0
92	94.0
93	26.0
94	14.5
95	3.0
96	3.0
97	3.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	455322.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.84698480947615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.46724300313306	69.2089433242964
2	4.60759620470173	6.897293662830209
3	1.034567190765254	2.3230270443476817
4	0.4752156090384026	1.4227382188369313
5	0.25611980837192433	0.9584897703309682
6	0.17205183582457673	0.7726536685442749
7	0.12913146001858672	0.6765570298505645
8	0.10129057815249727	0.6065035491458416
9	0.08021564755237882	0.5403509418451672
>10	0.5805288565197276	8.433744598072304
>50	0.06755093417680155	3.478077151444952
>100	0.027314072910252098	3.1808731960078696
>500	2.936997087123878E-4	0.1784982056087345
>1k	8.810991261371633E-4	1.322249638838101
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2398	0.526660253622711	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1984	0.4357355893192071	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1633	0.35864728697493203	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	812	0.17833533191894965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.196247930036326E-4	0.0
10	0.0	0.0	0.0	4.392495860072652E-4	0.0
11	0.0	0.0	0.0	4.392495860072652E-4	0.0
12	0.0	0.0	0.0	4.392495860072652E-4	0.0
13	0.0	0.0	0.0	4.392495860072652E-4	0.0
14	0.0	0.0	0.0	4.392495860072652E-4	0.0
15	0.0	0.0	0.0	4.392495860072652E-4	0.0
16	0.0	0.0	0.0	8.784991720145304E-4	0.0
17	0.0	0.0	0.0	0.001098123965018163	0.0
18	0.0	0.0	0.0	0.0013177487580217956	0.0
19	0.0	0.0	0.0	0.001537373551025428	0.0
20	0.0	0.0	0.0	0.001537373551025428	0.0
21	0.0	0.0	0.0	0.0017569983440290607	0.0
22	0.0	0.0	0.0	0.002855122309047224	0.0
23	0.0	0.0	0.0	0.0041728710670690195	0.0
24	0.0	0.0	0.0	0.007247618169119876	0.0
25	0.0	0.0	0.0	0.010102740478167099	0.0
26	0.0	0.0	0.0	0.016471859475272445	0.0
27	0.0	0.0	0.0	0.03294371895054489	0.0
28	0.0	0.0	0.0	0.09092466430350389	0.0
29	0.0	0.0	0.0	0.1691110906127971	0.0
30	0.0	0.0	0.0	0.2975915945199222	0.0
31	0.0	0.0	0.0	0.58222532625263	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGTC	35	8.8611373E-4	26.42857	5
TATAATA	70	6.5701897E-9	26.42857	2
CCTAGAC	50	9.075355E-6	25.899998	3
GGTATCA	815	0.0	23.60736	1
TATACGT	55	1.8993887E-5	23.545454	4
CGTGTTT	40	0.0019294057	23.125002	18
TATAATC	80	2.7166607E-8	23.125002	3
AACCGCG	60	3.7199265E-5	21.583334	7
GTCTCGC	45	0.0038223134	20.555555	1
ATATAAT	45	0.0038223134	20.555555	1
CGTCCAC	45	0.0038223134	20.555555	8
CTAGCGG	105	2.2504537E-8	19.38095	29
TCTAGCG	110	3.8364306E-8	18.5	28
TAATACC	50	0.007028519	18.499998	4
CTATACG	50	0.007028519	18.499998	3
TCGTGTT	50	0.007028519	18.499998	17
CGGTCCA	125	8.538336E-9	17.760002	10
AGGCCCG	95	3.5967169E-6	17.526316	10
TTTCGGA	75	2.0638134E-4	17.266666	30
TAGAACC	65	0.001577954	17.076923	4
>>END_MODULE