FastQCFastQC Report
Fri 10 Feb 2017
ERR1632105.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632105.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296048
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC6640.22428795330486948No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA6280.21212776306544887No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG5090.17193157866291953No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG3930.1327487434470086No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC3850.13004647894935956No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT3600.12160190239420635No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA3550.11991298708317569No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG3550.11991298708317569No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC3420.11552180727449601No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA3390.11450845808787764No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA3300.11146841052802248No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA3290.11113062746581634No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC3170.1070772307193428No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA3150.10640166459493054No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA3050.10302383397286927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGT200.001839728937.04
TCTAATA400.001927819223.1250022
GGTATCA1200.023.1249981
AAATCTC2450.022.653069
CCTTTCA2500.022.19999927
AGTACGG502.6955403E-422.19999936
CTTTCAC2550.021.76470628
TTCCTCG2550.021.76470619
GAAATCT2650.021.641518
ATCTCGC2600.021.34615311
GGGAAAT2600.021.3461536
TCTCGCC2600.021.34615312
CGGCCTT2700.021.24074224
TATTAAT450.003819199720.5555572
AATCTCG2700.020.55555510
CTAGATT555.1307055E-420.1818184
GGAAATC2750.020.1818187
TAGGACA1101.7389539E-920.1818184
GTACGGG555.1307055E-420.18181837
CTCGCCT2850.020.12280813
Produced by FastQC (version 0.11.5)