##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632105.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	296048
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.838874101497055	34.0	31.0	34.0	31.0	34.0
2	33.0198886667027	34.0	33.0	34.0	31.0	34.0
3	33.09991960763119	34.0	33.0	34.0	31.0	34.0
4	36.488055990920394	37.0	37.0	37.0	35.0	37.0
5	36.34980813922067	37.0	37.0	37.0	35.0	37.0
6	36.37601672701724	37.0	37.0	37.0	35.0	37.0
7	36.35357442036427	37.0	37.0	37.0	35.0	37.0
8	36.33806004431714	37.0	37.0	37.0	35.0	37.0
9	38.149773009782194	39.0	39.0	39.0	37.0	39.0
10	38.1218923958277	39.0	38.0	39.0	37.0	39.0
11	38.18706088201913	39.0	39.0	39.0	37.0	39.0
12	38.16730732854132	39.0	39.0	39.0	37.0	39.0
13	38.17696454628979	39.0	39.0	39.0	37.0	39.0
14	39.60831013889639	41.0	39.0	41.0	37.0	41.0
15	39.62418256498946	41.0	39.0	41.0	37.0	41.0
16	39.614224720315626	41.0	39.0	41.0	37.0	41.0
17	39.60200372912501	41.0	39.0	41.0	37.0	41.0
18	39.59478192725504	41.0	39.0	41.0	37.0	41.0
19	39.62911419769767	41.0	39.0	41.0	37.0	41.0
20	39.604922174782466	41.0	39.0	41.0	37.0	41.0
21	39.57208966113603	41.0	39.0	41.0	37.0	41.0
22	39.5496845106199	41.0	39.0	41.0	37.0	41.0
23	39.506796195211585	40.0	39.0	41.0	37.0	41.0
24	39.47276792952494	41.0	39.0	41.0	37.0	41.0
25	39.424444684645735	41.0	39.0	41.0	37.0	41.0
26	39.35188212722261	40.0	39.0	41.0	37.0	41.0
27	39.282815624493324	40.0	39.0	41.0	36.0	41.0
28	39.2377654974869	40.0	39.0	41.0	36.0	41.0
29	39.17621129006107	40.0	39.0	41.0	36.0	41.0
30	39.1126742960601	40.0	39.0	41.0	36.0	41.0
31	39.0552579311463	40.0	39.0	41.0	35.0	41.0
32	38.98310409122845	40.0	39.0	41.0	35.0	41.0
33	38.92473517807923	40.0	38.0	41.0	35.0	41.0
34	38.86349848673188	40.0	38.0	41.0	35.0	41.0
35	38.85610441550019	40.0	38.0	41.0	35.0	41.0
36	38.828065043506456	40.0	38.0	41.0	35.0	41.0
37	38.79792128303519	40.0	38.0	41.0	35.0	41.0
38	38.73817421499216	40.0	38.0	41.0	35.0	41.0
39	38.687935740150245	40.0	38.0	41.0	35.0	41.0
40	38.660960384802465	40.0	38.0	41.0	35.0	41.0
41	38.632917634978114	40.0	38.0	41.0	35.0	41.0
42	38.60932011025239	40.0	38.0	41.0	35.0	41.0
43	37.74689577365832	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	8.0
19	9.0
20	13.0
21	30.0
22	47.0
23	83.0
24	138.0
25	197.0
26	284.0
27	447.0
28	606.0
29	898.0
30	1376.0
31	1867.0
32	2739.0
33	3977.0
34	6081.0
35	9454.0
36	16001.0
37	29295.0
38	75168.0
39	147325.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57181267902502	17.12560125385073	12.69726530832838	27.60532075879587
2	18.980030265362373	20.196724855428847	33.6364373344863	27.18680754472248
3	19.72720639896233	20.682119115819056	30.23428633194617	29.356388153272444
4	15.754202021293844	14.932038047884127	32.28699400097281	37.02676592984921
5	16.1210344268497	35.177403664270656	33.01592984921364	15.685632059665998
6	36.916310868507814	32.996338431605686	15.027292871426257	15.060057828460248
7	31.24358212181808	29.232759552505	19.609657893314598	19.914000432362318
8	28.659541695941197	32.6774712208831	19.288763984218775	19.374223098956925
9	27.294560341566232	14.196008755336972	17.772455817975462	40.73697508512133
10	17.634640328595363	26.429835702318545	31.22399070421013	24.711533264875964
11	37.946886991298705	20.095727719829217	20.339944873804246	21.617440415067826
12	23.621845106198993	23.48774523050316	26.916581094957575	25.973828568340267
13	32.02318542938983	17.58228395395341	23.388774793276763	27.00575582337999
14	24.330176187645247	18.57806842133708	22.721653245419663	34.37010214559801
15	27.23274604118251	25.81878614278766	20.48282710911744	26.465640706912392
16	27.72692266119008	23.922134248500242	21.41037939793547	26.94056369237421
17	26.42037777657677	24.683497270712856	22.710168621304653	26.18595633140572
18	25.670161595416957	23.200629627627954	24.218707777117224	26.910500999837865
19	27.443522671999137	23.416473004377668	23.872480138355943	25.267524185267256
20	27.460749608171646	22.775698535372644	23.710006485434796	26.053545371020913
21	27.239501702426633	23.853564286872402	22.203156244933254	26.70377776576771
22	27.237137220991194	23.281697562557426	22.42507971680268	27.05608549964871
23	26.12718207858185	23.771820785818516	22.976679457385288	27.124317678214343
24	27.760363184348485	23.16583797222072	22.74090687996541	26.332891963465382
25	27.23882613630222	22.582824406852943	23.143544290115116	27.03480516672972
26	27.023996108739123	22.68686159001243	23.183064908393234	27.10607739285521
27	26.56697562557423	22.698008431065233	23.42761984543047	27.307396097930063
28	26.077527968437554	23.162797924660865	23.601578122466627	27.158095984434954
29	26.067056693509162	23.9859752472572	23.36411662973572	26.582851429497918
30	25.548559693022753	24.63789655731503	23.109765983894505	26.70377776576771
31	26.77369885964438	23.441131167918716	23.461060368588875	26.324109603848022
32	25.327987353402147	23.647854401988866	23.888693725341838	27.135464519267146
33	25.89140950116197	22.875006755661246	23.941050099983787	27.292533643192996
34	26.14812462843863	23.015186726476788	24.068394314435494	26.768294330649084
35	25.784670053504836	22.793938820731775	24.355847700372912	27.065543425390477
36	25.392166135221316	23.18509160676647	24.770307517699834	26.65243474031238
37	26.511579203372428	21.23946116845917	24.788547803058965	27.460411825109443
38	24.886167108036535	21.865373182727126	26.32985191590553	26.91860779333081
39	24.892247203156245	21.137450683672917	26.859833540506944	27.110468572663894
40	24.09271469491434	21.070569637356105	28.147462573636705	26.68925309409285
41	23.23305680159974	21.180349132573095	28.931457061017134	26.65513700481003
42	23.589080149165	20.244690050262122	28.9517240447495	27.21450575582338
43	21.967721450575585	20.204831648921797	29.965073231367885	27.862373669134733
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	4.5
4	8.0
5	8.0
6	14.0
7	20.0
8	20.0
9	20.0
10	26.0
11	32.0
12	32.0
13	61.0
14	90.0
15	177.5
16	265.0
17	297.5
18	330.0
19	330.0
20	393.5
21	457.0
22	586.0
23	715.0
24	918.0
25	1121.0
26	1121.0
27	1401.5
28	1682.0
29	2424.5
30	3167.0
31	3766.5
32	4366.0
33	4366.0
34	5261.0
35	6156.0
36	7010.0
37	7864.0
38	9923.0
39	11982.0
40	11982.0
41	13889.5
42	15797.0
43	15649.0
44	15501.0
45	17439.0
46	19377.0
47	19377.0
48	21086.0
49	22795.0
50	25111.5
51	27428.0
52	29838.5
53	32249.0
54	32249.0
55	28293.0
56	24337.0
57	23176.0
58	22015.0
59	20119.5
60	18224.0
61	18224.0
62	17288.5
63	16353.0
64	14774.5
65	13196.0
66	11531.5
67	9867.0
68	9867.0
69	8519.5
70	7172.0
71	6252.0
72	5332.0
73	4325.5
74	3319.0
75	3319.0
76	2664.5
77	2010.0
78	1677.5
79	1345.0
80	1038.0
81	731.0
82	731.0
83	559.0
84	387.0
85	282.0
86	177.0
87	133.0
88	89.0
89	89.0
90	74.0
91	59.0
92	33.0
93	7.0
94	4.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	296048.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.56884204645894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.9800455021854	58.09907345363797
2	5.476089041118229	7.071694566566154
3	1.5349583796930117	2.9733145549885984
4	0.7169900499419173	1.8518086913432943
5	0.43840024950166273	1.4153498231600525
6	0.28473626868603386	1.1031054694611968
7	0.2135384715572374	0.965155228857507
8	0.15944376963149487	0.8236079661302385
9	0.12233179042525914	0.7108939847906168
>10	0.8880423626793352	11.40654201492855
>50	0.1126163407762543	4.994341382597891
>100	0.07123638300265388	7.975997347179932
>500	0.0015713908015291297	0.6091155163580145
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	664	0.22428795330486948	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	628	0.21212776306544887	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	509	0.17193157866291953	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	393	0.1327487434470086	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	385	0.13004647894935956	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	360	0.12160190239420635	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	355	0.11991298708317569	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	355	0.11991298708317569	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	342	0.11552180727449601	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	339	0.11450845808787764	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	330	0.11146841052802248	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	329	0.11113062746581634	No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC	317	0.1070772307193428	No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA	315	0.10640166459493054	No Hit
TTGTAATGCTGGTACAAGGCAGCTGGCAACGTTCCCTTCAAGA	305	0.10302383397286927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.3778306220612873E-4	0.0
3	0.0	0.0	0.0	3.3778306220612873E-4	0.0
4	0.0	0.0	0.0	3.3778306220612873E-4	0.0
5	0.0	0.0	0.0	3.3778306220612873E-4	0.0
6	0.0	0.0	0.0	3.3778306220612873E-4	0.0
7	0.0	0.0	0.0	3.3778306220612873E-4	0.0
8	0.0	0.0	0.0	3.3778306220612873E-4	0.0
9	0.0	0.0	0.0	0.0010133491866183863	0.0
10	0.0	0.0	0.0	0.001351132248824515	0.0
11	0.0	0.0	0.0	0.001351132248824515	0.0
12	0.0	0.0	0.0	0.001351132248824515	0.0
13	0.0	0.0	0.0	0.001351132248824515	0.0
14	0.0	0.0	0.0	0.001351132248824515	0.0
15	0.0	0.0	0.0	0.0016889153110306438	0.0
16	0.0	0.0	0.0	0.0016889153110306438	0.0
17	0.0	0.0	0.0	0.0020266983732367726	0.0
18	0.0	3.3778306220612873E-4	0.0	0.0020266983732367726	0.0
19	0.0	3.3778306220612873E-4	0.0	0.0023644814354429012	0.0
20	0.0	3.3778306220612873E-4	0.0	0.00270226449764903	0.0
21	0.0	3.3778306220612873E-4	0.0	0.00270226449764903	0.0
22	0.0	3.3778306220612873E-4	0.0	0.00270226449764903	0.0
23	0.0	3.3778306220612873E-4	0.0	0.0033778306220612875	0.0
24	0.0	3.3778306220612873E-4	0.0	0.0047289628708858025	0.0
25	0.0	3.3778306220612873E-4	0.0	0.006417878181916446	0.0
26	0.0	3.3778306220612873E-4	0.0	0.008444576555153219	0.0
27	0.0	3.3778306220612873E-4	0.0	0.022631465167810626	0.0
28	0.0	3.3778306220612873E-4	0.0	0.10471274928389991	0.0
29	0.0	3.3778306220612873E-4	0.0	0.2364481435442901	0.0
30	0.0	3.3778306220612873E-4	0.0	0.3918283521591093	0.0
31	0.0	3.3778306220612873E-4	0.0	0.8654002053721018	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGGT	20	0.0018397289	37.0	4
TCTAATA	40	0.0019278192	23.125002	2
GGTATCA	120	0.0	23.124998	1
AAATCTC	245	0.0	22.65306	9
CCTTTCA	250	0.0	22.199999	27
AGTACGG	50	2.6955403E-4	22.199999	36
CTTTCAC	255	0.0	21.764706	28
TTCCTCG	255	0.0	21.764706	19
GAAATCT	265	0.0	21.64151	8
ATCTCGC	260	0.0	21.346153	11
GGGAAAT	260	0.0	21.346153	6
TCTCGCC	260	0.0	21.346153	12
CGGCCTT	270	0.0	21.240742	24
TATTAAT	45	0.0038191997	20.555557	2
AATCTCG	270	0.0	20.555555	10
CTAGATT	55	5.1307055E-4	20.181818	4
GGAAATC	275	0.0	20.181818	7
TAGGACA	110	1.7389539E-9	20.181818	4
GTACGGG	55	5.1307055E-4	20.181818	37
CTCGCCT	285	0.0	20.122808	13
>>END_MODULE