FastQCFastQC Report
Fri 10 Feb 2017
ERR1632104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9472770
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT136410.14400222954848474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110540.11669237192500187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110380.11652346673676232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40950.027.4224681
CTTATAC72450.018.7936537
TCTTATA127700.015.54463737
GTATCAA72500.015.4379312
CGAATTA5350.014.52336415
GACGGAC12950.014.2857147
AAGACGG13500.014.1148155
CGTTACG1051.6576298E-414.0952382
CGTAGCG3350.013.25373115
TATACTG10100.013.1881195
ATCAACG110550.013.1700592
TCTAGCG9750.012.90256428
TCAACGC112900.012.8631543
GTACTAG4100.012.6341471
ATTCGCG2204.096364E-912.61363630
CAACGCA114600.012.6077674
TACGTTA5300.012.56603819
CTAGCGG10250.012.45365829
ATCTCGC15250.012.37377111
TAGGACA22300.012.1950674
Produced by FastQC (version 0.11.5)