##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632104.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9472770
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85945325390567	34.0	31.0	34.0	31.0	34.0
2	33.01319994046092	34.0	33.0	34.0	31.0	34.0
3	33.1006700257686	34.0	33.0	34.0	31.0	34.0
4	36.47117052351108	37.0	37.0	37.0	35.0	37.0
5	36.360397539473674	37.0	37.0	37.0	35.0	37.0
6	36.389595757101674	37.0	37.0	37.0	35.0	37.0
7	36.36800165104822	37.0	37.0	37.0	35.0	37.0
8	36.3550593965651	37.0	37.0	37.0	35.0	37.0
9	38.14788039823621	39.0	39.0	39.0	37.0	39.0
10	38.12606354846576	39.0	38.0	39.0	37.0	39.0
11	38.188634053186135	39.0	39.0	39.0	37.0	39.0
12	38.16046763512679	39.0	38.0	39.0	37.0	39.0
13	38.169778005799785	39.0	39.0	39.0	37.0	39.0
14	39.613937211607585	41.0	39.0	41.0	37.0	41.0
15	39.63245513192023	41.0	39.0	41.0	37.0	41.0
16	39.63272717483904	41.0	39.0	41.0	37.0	41.0
17	39.62179679227934	41.0	39.0	41.0	37.0	41.0
18	39.61918203439965	41.0	39.0	41.0	37.0	41.0
19	39.654710501785644	41.0	40.0	41.0	37.0	41.0
20	39.6348611863267	41.0	39.0	41.0	37.0	41.0
21	39.61284893436661	41.0	39.0	41.0	37.0	41.0
22	39.580367727707944	41.0	39.0	41.0	37.0	41.0
23	39.53888925836899	41.0	39.0	41.0	37.0	41.0
24	39.50904645631637	41.0	39.0	41.0	37.0	41.0
25	39.46338082736095	41.0	39.0	41.0	37.0	41.0
26	39.39431655154723	41.0	39.0	41.0	37.0	41.0
27	39.32955059607696	40.0	39.0	41.0	36.0	41.0
28	39.27729850930615	40.0	39.0	41.0	36.0	41.0
29	39.22727407083673	40.0	39.0	41.0	36.0	41.0
30	39.16947292080352	40.0	39.0	41.0	36.0	41.0
31	39.109673094564734	40.0	39.0	41.0	36.0	41.0
32	39.03833123785334	40.0	39.0	41.0	35.0	41.0
33	38.9778944279234	40.0	39.0	41.0	35.0	41.0
34	38.91020050101501	40.0	38.0	41.0	35.0	41.0
35	38.884900720697324	40.0	38.0	41.0	35.0	41.0
36	38.85105190984263	40.0	38.0	41.0	35.0	41.0
37	38.80478994000699	40.0	38.0	41.0	35.0	41.0
38	38.7618626864159	40.0	38.0	41.0	35.0	41.0
39	38.71295882830471	40.0	38.0	41.0	35.0	41.0
40	38.655960822441585	40.0	38.0	41.0	35.0	41.0
41	38.61461874404213	40.0	38.0	41.0	35.0	41.0
42	38.565386154208326	40.0	38.0	41.0	35.0	41.0
43	37.753144750690666	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	4.0
11	9.0
12	4.0
13	1.0
14	6.0
15	11.0
16	22.0
17	54.0
18	113.0
19	218.0
20	434.0
21	816.0
22	1522.0
23	2752.0
24	4395.0
25	7128.0
26	10531.0
27	16105.0
28	23362.0
29	34129.0
30	48641.0
31	67546.0
32	92361.0
33	128387.0
34	190764.0
35	287013.0
36	463686.0
37	878979.0
38	2276121.0
39	4937650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2696951366918	18.422932257407286	12.571729282986919	26.735643322913994
2	17.7145122282078	20.248290626712144	35.90472480594377	26.13247233913628
3	18.964062254229756	22.208762590034382	30.282515040479186	28.544660115256676
4	14.246614242718866	15.469540588444563	35.361884644090374	34.92196052474619
5	14.99753503990913	35.707411876357185	34.275423133887976	15.019629949845717
6	34.1382298947404	35.44594664496235	16.074601199015706	14.341222261281548
7	29.55679278605941	30.46067834434912	21.39667700155287	18.5858518680386
8	27.01836949487848	34.062602596706135	20.215301332134107	18.703726576281277
9	27.027511488191948	14.643245851002398	19.51029107642221	38.81895158438345
10	16.66000546830547	26.634247427098938	33.166813930877666	23.53893317371793
11	35.91600978383303	21.465178611958276	21.908280260156214	20.71053134405248
12	21.974374971629206	25.007458219718202	29.557806217188848	23.460360591463743
13	30.297009216944993	19.303276655086105	24.909567106559116	25.490147021409786
14	22.99566019231967	20.12221346026558	25.1359950679685	31.746131279446242
15	25.132173588084584	27.23716505309429	22.223098417886213	25.407562940934913
16	25.12395001673217	26.13127944624434	23.76222583257062	24.98254470445287
17	23.985275690215214	26.132366773393635	25.384602391908594	24.497755144482554
18	24.092836625401016	25.10681669670012	26.31797246212037	24.48237421577849
19	24.815381350967037	25.153719556159395	26.102227753867137	23.928671339006435
20	25.25643502375757	24.812942782311826	25.64619430219461	24.284427891735998
21	25.26972575075717	25.18972803097721	24.9794621847675	24.56108403349812
22	25.32815638931379	24.967670491313523	25.17828470447398	24.525888414898706
23	24.685947193904212	24.980665634233702	25.409019748183475	24.924367423678607
24	24.74275212002403	25.157361574280806	25.416821056565293	24.683065249129875
25	24.992879590658276	24.579589708184617	25.543943323864088	24.883587377293022
26	24.540942089800556	25.230444737917207	25.52432920887977	24.704283963402467
27	24.758270284193536	24.93681362473701	25.312606555421485	24.99230953564797
28	24.428419564710214	25.10492706990669	25.74497216759195	24.721681197791142
29	24.144912206250126	25.386059199157163	25.88340052592853	24.58562806866418
30	24.09077809341935	25.897451326275206	25.51653845707222	24.495232123233226
31	24.718355876897675	25.30333788321684	25.312733234312667	24.665573005572817
32	23.959591545028537	25.130695667687487	25.707654677565273	25.2020581097187
33	24.017008752455723	24.853342792023874	26.081199057931315	25.04844939758909
34	24.737948878733466	24.368479336033705	25.989831907667977	24.903739877564853
35	24.447822548209235	24.55176257842215	26.43731453418588	24.563100339182732
36	24.065421202034884	24.755588914330232	26.127426296637623	25.051563586997254
37	24.5192588862603	23.6338156632115	26.486645405726094	25.360280044802103
38	23.767292988217807	23.921851792031266	27.176496420793494	25.134358798957436
39	23.768158627307535	23.664651416639483	27.46692889197141	25.100261064081575
40	23.30065017940898	23.500718374878733	28.233283400737058	24.96534804497523
41	22.752172806898088	23.60471118796297	28.64804064703355	24.99507535810539
42	23.0071035188229	23.56911441954149	28.425708636438973	24.998073425196644
43	22.34070921177227	23.126572269779587	28.80483744459118	25.727881073856963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	531.0
1	597.5
2	664.0
3	1718.0
4	2772.0
5	2772.0
6	3423.5
7	4075.0
8	3844.0
9	3613.0
10	5276.0
11	6939.0
12	6939.0
13	11182.5
14	15426.0
15	24094.5
16	32763.0
17	35386.0
18	38009.0
19	38009.0
20	47514.0
21	57019.0
22	66530.5
23	76042.0
24	92939.0
25	109836.0
26	109836.0
27	131008.0
28	152180.0
29	186492.5
30	220805.0
31	252066.0
32	283327.0
33	283327.0
34	319791.5
35	356256.0
36	395122.0
37	433988.0
38	469744.0
39	505500.0
40	505500.0
41	542332.5
42	579165.0
43	582008.0
44	584851.0
45	605592.5
46	626334.0
47	626334.0
48	638476.5
49	650619.0
50	664832.0
51	679045.0
52	705209.5
53	731374.0
54	731374.0
55	694005.0
56	656636.0
57	629335.0
58	602034.0
59	557721.5
60	513409.0
61	513409.0
62	475485.0
63	437561.0
64	391838.0
65	346115.0
66	302451.5
67	258788.0
68	258788.0
69	220095.5
70	181403.0
71	158252.0
72	135101.0
73	107210.5
74	79320.0
75	79320.0
76	62350.5
77	45381.0
78	36795.5
79	28210.0
80	22338.5
81	16467.0
82	16467.0
83	12734.5
84	9002.0
85	7602.0
86	6202.0
87	5053.0
88	3904.0
89	3904.0
90	2837.0
91	1770.0
92	1001.5
93	233.0
94	148.5
95	64.0
96	64.0
97	43.0
98	22.0
99	18.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9472770.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.88698484652067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.55311193914835	42.834320682197145
2	9.898165389923738	10.271719151906568
3	2.9859728670129186	4.647993867084636
4	1.3061190558106284	2.710823186263918
5	0.7585590668835385	1.9679671404288512
6	0.45131105651636977	1.4050301970319268
7	0.3288051722308127	1.194249629229846
8	0.2424545033613874	1.0064186513506403
9	0.18326466358119511	0.8558145739926162
>10	1.0810103453166806	10.466231156789856
>50	0.1050952055179026	3.8130689932637316
>100	0.08886884472768561	9.526593077867874
>500	0.011901919315114818	4.29703864870169
>1k	0.005299061911964312	4.626299258889254
>5k	0.0	0.0
>10k+	6.090874173890933E-5	0.37643178500122315
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13641	0.14400222954848474	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11054	0.11669237192500187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11038	0.11652346673676232	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0556574264972125E-5
2	0.0	1.0556574264972125E-5	0.0	0.0	1.0556574264972125E-5
3	0.0	1.0556574264972125E-5	0.0	0.0	1.0556574264972125E-5
4	0.0	2.111314852994425E-5	0.0	0.0	1.0556574264972125E-5
5	0.0	6.333944558983276E-5	0.0	0.0	1.0556574264972125E-5
6	0.0	6.333944558983276E-5	0.0	0.0	1.0556574264972125E-5
7	0.0	6.333944558983276E-5	0.0	0.0	1.0556574264972125E-5
8	0.0	6.333944558983276E-5	0.0	0.0	1.0556574264972125E-5
9	1.0556574264972125E-5	6.333944558983276E-5	0.0	1.0556574264972125E-5	1.0556574264972125E-5
10	1.0556574264972125E-5	6.333944558983276E-5	0.0	5.278287132486063E-5	1.0556574264972125E-5
11	1.0556574264972125E-5	6.333944558983276E-5	0.0	7.389601985480488E-5	1.0556574264972125E-5
12	1.0556574264972125E-5	6.333944558983276E-5	0.0	1.4779203970960976E-4	1.0556574264972125E-5
13	1.0556574264972125E-5	6.333944558983276E-5	0.0	1.583486139745819E-4	1.0556574264972125E-5
14	1.0556574264972125E-5	6.333944558983276E-5	0.0	2.0057491103447038E-4	1.0556574264972125E-5
15	1.0556574264972125E-5	6.333944558983276E-5	0.0	2.850275051542474E-4	1.0556574264972125E-5
16	1.0556574264972125E-5	8.4452594119777E-5	0.0	4.22262970598885E-4	1.0556574264972125E-5
17	1.0556574264972125E-5	8.4452594119777E-5	0.0	6.228378816333554E-4	1.0556574264972125E-5
18	1.0556574264972125E-5	8.4452594119777E-5	0.0	7.072904757531324E-4	1.0556574264972125E-5
19	1.0556574264972125E-5	1.0556574264972126E-4	0.0	8.022996441378815E-4	1.0556574264972125E-5
20	1.0556574264972125E-5	1.0556574264972126E-4	0.0	9.28978535317547E-4	1.0556574264972125E-5
21	1.0556574264972125E-5	1.0556574264972126E-4	0.0	0.0012351191890017388	4.22262970598885E-5
22	1.0556574264972125E-5	1.0556574264972126E-4	0.0	0.0016996084566605122	4.22262970598885E-5
23	1.0556574264972125E-5	1.3723546544463762E-4	0.0	0.0025019081007983937	4.22262970598885E-5
24	1.0556574264972125E-5	1.4779203970960976E-4	0.0	0.003694800992740244	4.22262970598885E-5
25	1.0556574264972125E-5	1.4779203970960976E-4	0.0	0.004518213785408069	4.22262970598885E-5
26	1.0556574264972125E-5	1.4779203970960976E-4	0.0	0.005964464459709251	4.22262970598885E-5
27	1.0556574264972125E-5	1.583486139745819E-4	0.0	0.017808940785007974	4.22262970598885E-5
28	1.0556574264972125E-5	1.7946176250452613E-4	0.0	0.07090850933781777	4.22262970598885E-5
29	1.0556574264972125E-5	1.9001833676949824E-4	0.0	0.14806651064049903	4.22262970598885E-5
30	1.0556574264972125E-5	1.9001833676949824E-4	0.0	0.252988302260057	4.22262970598885E-5
31	1.0556574264972125E-5	1.9001833676949824E-4	0.0	0.6396228347146611	4.22262970598885E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4095	0.0	27.422468	1
CTTATAC	7245	0.0	18.79365	37
TCTTATA	12770	0.0	15.544637	37
GTATCAA	7250	0.0	15.437931	2
CGAATTA	535	0.0	14.523364	15
GACGGAC	1295	0.0	14.285714	7
AAGACGG	1350	0.0	14.114815	5
CGTTACG	105	1.6576298E-4	14.095238	2
CGTAGCG	335	0.0	13.253731	15
TATACTG	1010	0.0	13.188119	5
ATCAACG	11055	0.0	13.170059	2
TCTAGCG	975	0.0	12.902564	28
TCAACGC	11290	0.0	12.863154	3
GTACTAG	410	0.0	12.634147	1
ATTCGCG	220	4.096364E-9	12.613636	30
CAACGCA	11460	0.0	12.607767	4
TACGTTA	530	0.0	12.566038	19
CTAGCGG	1025	0.0	12.453658	29
ATCTCGC	1525	0.0	12.373771	11
TAGGACA	2230	0.0	12.195067	4
>>END_MODULE