FastQCFastQC Report
Fri 10 Feb 2017
ERR1632103.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632103.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6585561
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT173120.26287813597049664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT131500.1996792680228761No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC128690.195412357428623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127440.1935142655272649No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA114740.1742296518094662No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG92890.14105100537372595No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA91740.13930476082447646No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC85140.12928283558530548No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA82790.1257144228107522No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT74080.1124885184420887No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG73210.11116744647874342No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA71800.10902639881401144No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37650.028.7450181
CTTATAC74450.019.73002237
TCGCCAT30100.016.10299113
CGGCCTT39250.016.0726124
ATCTCGC40350.015.95539111
GTATCAA73350.015.7382421
TCTCGCC40650.015.56457612
TTCCTCG41700.015.26139119
TAGACCG855.3689384E-415.2352935
TATAGCG750.00410714814.82
TACGTTA3750.014.79999919
AGGTCGC32450.014.76579410
GTCGCCA32650.014.73200612
TATACAC21750.014.62988537
GGCCTTT43500.014.54482725
GAGGTCG33750.014.5259269
TCTTATA121250.014.49484437
AAATCTC44000.014.3375019
TAACGCC4000.014.3375014
TCGGCCT45000.014.30666723
Produced by FastQC (version 0.11.5)