##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632103.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6585561
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.87754163996051	34.0	31.0	34.0	31.0	34.0
2	33.037016588260286	34.0	33.0	34.0	31.0	34.0
3	33.123537539170925	34.0	33.0	34.0	31.0	34.0
4	36.48864994189561	37.0	37.0	37.0	35.0	37.0
5	36.37506979283921	37.0	37.0	37.0	35.0	37.0
6	36.40483384786808	37.0	37.0	37.0	35.0	37.0
7	36.38247037723893	37.0	37.0	37.0	35.0	37.0
8	36.371850173432456	37.0	37.0	37.0	35.0	37.0
9	38.16642743116342	39.0	39.0	39.0	37.0	39.0
10	38.15602057288665	39.0	38.0	39.0	37.0	39.0
11	38.21478504261064	39.0	39.0	39.0	37.0	39.0
12	38.19141786098405	39.0	39.0	39.0	37.0	39.0
13	38.199221903798325	39.0	39.0	39.0	37.0	39.0
14	39.634525441340536	41.0	39.0	41.0	37.0	41.0
15	39.661353375969036	41.0	39.0	41.0	37.0	41.0
16	39.65574231261392	41.0	39.0	41.0	37.0	41.0
17	39.65401079725782	41.0	39.0	41.0	37.0	41.0
18	39.64933784684403	41.0	39.0	41.0	37.0	41.0
19	39.69398294237955	41.0	40.0	41.0	38.0	41.0
20	39.6760916799647	41.0	40.0	41.0	37.0	41.0
21	39.65167204434064	41.0	39.0	41.0	37.0	41.0
22	39.62423170934109	41.0	39.0	41.0	37.0	41.0
23	39.58022422083707	41.0	39.0	41.0	37.0	41.0
24	39.55591998920062	41.0	39.0	41.0	37.0	41.0
25	39.50786273181586	41.0	39.0	41.0	37.0	41.0
26	39.4378267849922	41.0	39.0	41.0	37.0	41.0
27	39.371827396329635	41.0	39.0	41.0	37.0	41.0
28	39.32222752169481	40.0	39.0	41.0	36.0	41.0
29	39.27042358274413	40.0	39.0	41.0	36.0	41.0
30	39.208963518825506	40.0	39.0	41.0	36.0	41.0
31	39.13715232460834	40.0	39.0	41.0	36.0	41.0
32	39.063112770498975	40.0	39.0	41.0	35.0	41.0
33	38.99754159136936	40.0	39.0	41.0	35.0	41.0
34	38.92635221205908	40.0	38.0	41.0	35.0	41.0
35	38.91481682426144	40.0	38.0	41.0	35.0	41.0
36	38.88163999999393	40.0	38.0	41.0	35.0	41.0
37	38.84252321100662	40.0	38.0	41.0	35.0	41.0
38	38.79360087925691	40.0	38.0	41.0	35.0	41.0
39	38.7480457625402	40.0	38.0	41.0	35.0	41.0
40	38.693745301273495	40.0	38.0	41.0	35.0	41.0
41	38.65406591784664	40.0	38.0	41.0	35.0	41.0
42	38.61197945019414	40.0	38.0	41.0	35.0	41.0
43	37.81405243987566	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	6.0
11	4.0
12	4.0
13	4.0
14	7.0
15	7.0
16	7.0
17	42.0
18	83.0
19	156.0
20	240.0
21	521.0
22	955.0
23	1638.0
24	2745.0
25	4440.0
26	6843.0
27	10017.0
28	14973.0
29	22019.0
30	31786.0
31	44058.0
32	61062.0
33	85434.0
34	128038.0
35	194979.0
36	318474.0
37	622401.0
38	1569803.0
39	3464812.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.28768057876922	18.88176269265443	12.898202598077827	25.932354130498524
2	17.196439301070935	21.07425624028082	36.33723839168751	25.392066066960734
3	18.62936202397943	22.63887617167315	31.96485462665975	26.76690717768767
4	14.377727273348468	15.897810376367328	35.454580103350345	34.26988224693386
5	14.334875950583406	36.68222039094316	34.46442907445547	14.518474584017977
6	33.080750447835804	35.925139862799846	16.574320699481788	14.419788989882562
7	28.470027078938305	31.804063465511902	21.33441630864857	18.391493146901226
8	26.58429555204181	34.7963977556354	20.484754449924615	18.13455224239818
9	26.27329699018808	15.431639005393768	19.828500563581446	38.4665634408367
10	16.33207861866286	27.556316007094917	33.30150612833136	22.810099245910862
11	34.91476276660409	22.08387409971603	22.740036877647935	20.261326256031946
12	21.5137632162241	25.738612093943097	29.423674004386264	23.32395068544654
13	29.665293511061545	19.75711712335517	25.492163841470756	25.085425524112527
14	22.285497013845898	20.686605135082644	25.606094909757875	31.42180294131358
15	24.716345350077237	27.817159388547157	23.116739788759073	24.349755472616533
16	24.686856594297737	26.86846268677794	23.96808715309144	24.47659356583289
17	23.84176534087225	26.82462435622417	25.507409315622464	23.826200987281112
18	22.935130355637128	25.67274071259836	26.964445398045818	24.427683533718692
19	24.331290834600118	25.870658551336778	27.16939376918686	22.62865684487624
20	24.603431659049242	25.196000765918043	26.888066179935166	23.31250139509755
21	24.222522576284693	26.398236991503076	25.414706507160133	23.964533925052095
22	24.63352780423718	25.73094380266161	25.56081402935908	24.07471436374213
23	23.82594284678253	26.079828278866447	25.981112315260614	24.11311655909041
24	25.05183688982609	25.472833673547324	25.777485016082913	23.69784442054367
25	24.486661045277692	24.728994234507887	26.450654697450982	24.333690022763438
26	24.093391588051496	25.085562186729423	26.300523220421162	24.52052300479792
27	23.984031125062845	25.428069074145693	26.039634284763284	24.548265516028174
28	23.421208914472132	26.03756916077461	26.42865201613044	24.11256990862282
29	23.711616975379926	26.369932645070026	26.262667675540474	23.655782704009575
30	23.240966107519164	26.93173747840161	25.829173854740695	23.998122559338526
31	23.926769488582675	25.820518555670503	26.36776122793487	23.88495072781195
32	23.00765265100422	26.04037833678862	26.456394527360693	24.49557448484647
33	23.421072251855232	25.36600906133889	26.926999841015824	24.285918845790057
34	23.725131389717596	25.035057757418084	26.743067143406613	24.496743709457704
35	23.580648634186215	25.167635680544148	26.77043003625659	24.481285649013046
36	23.351510979854257	25.06040108048502	27.07597727816962	24.5121106614911
37	24.004257192363717	23.498742172458808	27.080821208701888	25.416179426475587
38	22.926262470273983	24.211179579082177	28.360939941183442	24.501618009460394
39	22.80054804746323	23.627842791221585	28.91577498105325	24.65583418026194
40	22.63942282214074	23.30448385490621	29.55502196396025	24.501071358992803
41	21.688129530650464	23.829921247407775	30.0861688169011	24.395780405040664
42	22.041706090035458	22.99661334850592	29.82107370958981	25.140606851868807
43	20.866665725213085	23.13403520216425	30.277375002676308	25.721924069946354
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	451.0
1	528.0
2	605.0
3	1567.5
4	2530.0
5	2530.0
6	3358.0
7	4186.0
8	4200.0
9	4214.0
10	6281.0
11	8348.0
12	8348.0
13	12597.5
14	16847.0
15	29350.5
16	41854.0
17	42945.5
18	44037.0
19	44037.0
20	48649.0
21	53261.0
22	61444.5
23	69628.0
24	80396.5
25	91165.0
26	91165.0
27	103024.5
28	114884.0
29	150544.5
30	186205.0
31	197868.5
32	209532.0
33	209532.0
34	231936.5
35	254341.0
36	271944.0
37	289547.0
38	330658.5
39	371770.0
40	371770.0
41	409389.5
42	447009.0
43	427596.0
44	408183.0
45	426664.0
46	445145.0
47	445145.0
48	457040.5
49	468936.0
50	486437.5
51	503939.0
52	525479.0
53	547019.0
54	547019.0
55	479324.5
56	411630.0
57	385929.0
58	360228.0
59	328729.5
60	297231.0
61	297231.0
62	275975.0
63	254719.0
64	227391.5
65	200064.0
66	175259.0
67	150454.0
68	150454.0
69	130234.5
70	110015.0
71	95634.5
72	81254.0
73	67481.5
74	53709.0
75	53709.0
76	43543.0
77	33377.0
78	27512.0
79	21647.0
80	17100.0
81	12553.0
82	12553.0
83	9867.0
84	7181.0
85	5682.0
86	4183.0
87	3261.5
88	2340.0
89	2340.0
90	1688.0
91	1036.0
92	631.0
93	226.0
94	143.0
95	60.0
96	60.0
97	38.5
98	17.0
99	9.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6585561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.42435197935688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.61086526613519	36.61696317209438
2	11.22479683662532	10.197582448072803
3	3.318233810821499	4.521858617176754
4	1.4670767740656594	2.665640470635917
5	0.7743777179145478	1.7587803011760776
6	0.4780761324296566	1.3029779107448611
7	0.3330981004195737	1.0591535749979715
8	0.2617759359488552	0.9512801803413101
9	0.19015069018581376	0.7773724692106241
>10	1.0981469910202808	9.420918455764497
>50	0.115880775802948	3.7038087894539564
>100	0.10042356005936881	9.304740389336771
>500	0.014349636575795665	4.482052474110392
>1k	0.011813380331055445	10.018498669553638
>5k	7.675360101912904E-4	2.1944224583637797
>10k+	1.6685565438941095E-4	1.0239496189661446
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17312	0.26287813597049664	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13150	0.1996792680228761	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	12869	0.195412357428623	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12744	0.1935142655272649	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	11474	0.1742296518094662	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	9289	0.14105100537372595	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	9174	0.13930476082447646	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	8514	0.12928283558530548	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	8279	0.1257144228107522	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	7408	0.1124885184420887	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	7321	0.11116744647874342	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	7180	0.10902639881401144	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5184735210865103E-5	1.5184735210865103E-5
2	0.0	1.5184735210865103E-5	0.0	3.0369470421730206E-5	1.5184735210865103E-5
3	0.0	3.0369470421730206E-5	0.0	3.0369470421730206E-5	3.0369470421730206E-5
4	0.0	6.073894084346041E-5	0.0	3.0369470421730206E-5	3.0369470421730206E-5
5	0.0	7.592367605432552E-5	0.0	3.0369470421730206E-5	3.0369470421730206E-5
6	0.0	7.592367605432552E-5	0.0	3.0369470421730206E-5	3.0369470421730206E-5
7	0.0	7.592367605432552E-5	0.0	4.555420563259531E-5	3.0369470421730206E-5
8	0.0	7.592367605432552E-5	0.0	9.110841126519061E-5	9.110841126519061E-5
9	0.0	7.592367605432552E-5	0.0	1.6703208731951613E-4	9.110841126519061E-5
10	0.0	7.592367605432552E-5	0.0	2.5814049858470674E-4	1.0629314647605572E-4
11	0.0	7.592367605432552E-5	0.0	3.796183802716276E-4	1.0629314647605572E-4
12	0.0	7.592367605432552E-5	0.0	5.314657323802787E-4	1.0629314647605572E-4
13	0.0	7.592367605432552E-5	0.0	5.466504675911437E-4	1.0629314647605572E-4
14	0.0	7.592367605432552E-5	0.0	5.770199380128739E-4	1.0629314647605572E-4
15	0.0	7.592367605432552E-5	0.0	6.073894084346041E-4	1.0629314647605572E-4
16	0.0	7.592367605432552E-5	0.0	6.984978196997948E-4	1.3666261689778593E-4
17	0.0	7.592367605432552E-5	0.0	8.807146422301759E-4	1.3666261689778593E-4
18	0.0	7.592367605432552E-5	0.0	9.718230534953666E-4	1.3666261689778593E-4
19	0.0	7.592367605432552E-5	0.0	0.0010629314647605573	1.3666261689778593E-4
20	0.0	7.592367605432552E-5	0.0	0.0012451482872909384	1.3666261689778593E-4
21	0.0	7.592367605432552E-5	0.0	0.0014121803746104545	1.6703208731951613E-4
22	0.0	7.592367605432552E-5	0.0	0.0018829071661472729	1.6703208731951613E-4
23	0.0	7.592367605432552E-5	0.0	0.002535850780214472	1.6703208731951613E-4
24	0.0	7.592367605432552E-5	0.0	0.003811368537927141	1.6703208731951613E-4
25	0.0	7.592367605432552E-5	0.0	0.004980593149163754	1.6703208731951613E-4
26	0.0	7.592367605432552E-5	0.0	0.0065294361406719945	1.6703208731951613E-4
27	0.0	7.592367605432552E-5	0.0	0.026087375092266246	1.9740155774124635E-4
28	0.0	9.110841126519061E-5	0.0	0.108737888845005	1.9740155774124635E-4
29	0.0	9.110841126519061E-5	0.0	0.23692742349512821	1.9740155774124635E-4
30	0.0	9.110841126519061E-5	0.0	0.4132373840284829	1.9740155774124635E-4
31	0.0	9.110841126519061E-5	0.0	0.9935979637877471	1.9740155774124635E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3765	0.0	28.745018	1
CTTATAC	7445	0.0	19.730022	37
TCGCCAT	3010	0.0	16.102991	13
CGGCCTT	3925	0.0	16.07261	24
ATCTCGC	4035	0.0	15.955391	11
GTATCAA	7335	0.0	15.738242	1
TCTCGCC	4065	0.0	15.564576	12
TTCCTCG	4170	0.0	15.261391	19
TAGACCG	85	5.3689384E-4	15.235293	5
TATAGCG	75	0.004107148	14.8	2
TACGTTA	375	0.0	14.799999	19
AGGTCGC	3245	0.0	14.765794	10
GTCGCCA	3265	0.0	14.732006	12
TATACAC	2175	0.0	14.629885	37
GGCCTTT	4350	0.0	14.544827	25
GAGGTCG	3375	0.0	14.525926	9
TCTTATA	12125	0.0	14.494844	37
AAATCTC	4400	0.0	14.337501	9
TAACGCC	400	0.0	14.337501	4
TCGGCCT	4500	0.0	14.306667	23
>>END_MODULE