FastQCFastQC Report
Fri 10 Feb 2017
ERR1632102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90417
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT1190.1316124180187354TruSeq Adapter, Index 8 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG358.806073E-426.4285722
CCTATTA358.806073E-426.4285722
TTGGACA400.001917530523.1254
TCTCGTA400.001917530523.12530
TCGTATG400.001917530523.12532
GTATCAA400.001917530523.1251
ATCTCGT450.003799010520.55555729
CTCGTAT450.003799010520.55555731
TATGCCG450.003799010520.55555735
CCCACGA450.003799010520.55555711
GTATGCC450.003799010520.55555734
ATGCCGT450.003799010520.55555736
TATACAC450.003799010520.55555737
TGCCGTC450.003799010520.55555737
TATCAAC1052.1913365E-819.380951
ATCAACG1152.9413059E-919.3043482
CAACGCA1103.7362042E-818.54
CTTGGAC609.1473944E-418.53
TCAACGC1103.7362042E-818.53
CGTATGC500.00698608118.49999833
Produced by FastQC (version 0.11.5)