##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632102.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	90417
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78706437948616	34.0	31.0	34.0	31.0	34.0
2	32.95047391530354	34.0	31.0	34.0	31.0	34.0
3	33.03752612893593	34.0	33.0	34.0	31.0	34.0
4	36.42073946271166	37.0	37.0	37.0	35.0	37.0
5	36.28607452138425	37.0	37.0	37.0	35.0	37.0
6	36.318446752270034	37.0	37.0	37.0	35.0	37.0
7	36.297985998208304	37.0	37.0	37.0	35.0	37.0
8	36.28102016213765	37.0	37.0	37.0	35.0	37.0
9	38.097050333454995	39.0	39.0	39.0	37.0	39.0
10	38.0573343508411	39.0	38.0	39.0	35.0	39.0
11	38.152062112213414	39.0	39.0	39.0	37.0	39.0
12	38.10061161064844	39.0	38.0	39.0	37.0	39.0
13	38.11935808531582	39.0	38.0	39.0	37.0	39.0
14	39.51717044361127	40.0	39.0	41.0	37.0	41.0
15	39.52621741486667	40.0	39.0	41.0	37.0	41.0
16	39.502516119756244	40.0	39.0	41.0	37.0	41.0
17	39.50221750334561	40.0	39.0	41.0	37.0	41.0
18	39.50298063417278	40.0	39.0	41.0	37.0	41.0
19	39.52914827963768	41.0	39.0	41.0	37.0	41.0
20	39.51383036375903	41.0	39.0	41.0	37.0	41.0
21	39.48751894002234	41.0	39.0	41.0	37.0	41.0
22	39.45165179114547	40.0	39.0	41.0	37.0	41.0
23	39.39493679285975	40.0	39.0	41.0	37.0	41.0
24	39.36377008748355	40.0	39.0	41.0	37.0	41.0
25	39.30252054370307	40.0	39.0	41.0	36.0	41.0
26	39.23573000652532	40.0	39.0	41.0	36.0	41.0
27	39.16022429410398	40.0	39.0	41.0	36.0	41.0
28	39.09251578796023	40.0	39.0	41.0	36.0	41.0
29	39.053585055907625	40.0	39.0	41.0	36.0	41.0
30	38.98911709081257	40.0	39.0	41.0	35.0	41.0
31	38.943141223442495	40.0	38.0	41.0	35.0	41.0
32	38.86562261521617	40.0	38.0	41.0	35.0	41.0
33	38.78770585177566	40.0	38.0	41.0	35.0	41.0
34	38.72090425473086	40.0	38.0	41.0	35.0	41.0
35	38.709977106075186	40.0	38.0	41.0	35.0	41.0
36	38.67806938960594	40.0	38.0	41.0	35.0	41.0
37	38.645730338321336	40.0	38.0	41.0	35.0	41.0
38	38.60411205857306	40.0	38.0	41.0	35.0	41.0
39	38.59005496753929	40.0	38.0	41.0	35.0	41.0
40	38.5302874459449	40.0	38.0	41.0	35.0	41.0
41	38.49404426158797	40.0	38.0	41.0	35.0	41.0
42	38.4714157735824	40.0	38.0	41.0	35.0	41.0
43	37.6202705243483	39.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	2.0
19	1.0
20	3.0
21	10.0
22	21.0
23	38.0
24	57.0
25	74.0
26	101.0
27	173.0
28	250.0
29	333.0
30	457.0
31	695.0
32	916.0
33	1381.0
34	2024.0
35	3112.0
36	5135.0
37	9311.0
38	23194.0
39	43128.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.20998263600871	18.03643120209695	12.743178826990501	27.010407334903835
2	20.193105278874548	19.86794518729885	32.8655009566785	27.073448577148103
3	20.77706625966356	20.741674685070286	29.013349259541897	29.467909795724257
4	16.098742493115235	15.386487054425608	32.2207107070573	36.29405974540186
5	16.899476868288044	34.024575024608204	32.97056969375228	16.10537841335147
6	37.22419456518133	32.74384241901412	14.548149131247442	15.483813884557108
7	31.142373668668505	28.20708495083889	20.06259884756185	20.587942532930754
8	28.26680823296504	31.69979096851256	19.295044073570235	20.73835672495217
9	27.543492927215013	13.538383268633112	18.444540296625632	40.47358350752624
10	18.238826769302232	25.306081820896516	31.924306269838638	24.530785139962617
11	37.881150668568964	20.162137651105432	20.06038687414977	21.89632480617583
12	23.640465841600584	23.77207825961932	27.14423172633465	25.443224172445444
13	32.775916033489274	18.14371191258281	22.359733236006505	26.72063881792141
14	24.499817512193502	19.75292256987071	22.85632126701837	32.89093865091742
15	27.044692922791068	25.84801530685601	20.378911045489232	26.728380724863687
16	26.904232611124012	24.599356315737083	22.131899974562305	26.364511098576592
17	25.42774035856089	24.66682150480551	23.414844553568468	26.49059358306513
18	25.512901334925953	22.87622902772709	24.61594611632768	26.994923521019278
19	26.61778205425971	23.450236128161738	24.24875852992247	25.68322328765608
20	26.93298826548105	23.2323567470719	23.689129256666337	26.145525730780715
21	27.744782507714255	23.277702202019533	22.689317274406363	26.288198015859848
22	27.581096475220367	23.5464569715872	22.570976696860104	26.301469856332325
23	26.736122631805966	23.105168275877325	23.28986805578597	26.86884103653074
24	26.428658327526904	23.6714334693697	23.095214395522966	26.80469380758043
25	26.690777176858333	23.101850315759204	23.25115852107458	26.956213986307887
26	26.368935045400754	23.639359854894547	23.274384241901412	26.71732085780329
27	26.72063881792141	23.323047656967162	23.001205525509583	26.955107999601847
28	26.142207770662594	23.23346273377794	23.690235243372374	26.93409425218709
29	25.75290045013659	23.275490228607453	24.166915513675523	26.80469380758043
30	25.557140803167545	24.074012630368184	23.8771469966931	26.49169956977117
31	26.44856608823562	23.640465841600584	23.229038786953783	26.681929283210014
32	25.261842352654924	23.324153643673203	24.039727042480948	27.374276961190922
33	25.166727495935497	22.91383257573244	24.487651658427065	27.431788269904995
34	26.17538737184379	22.723602862293596	24.380370947941206	26.72063881792141
35	25.6920711813044	22.38406494353938	25.38681885043742	26.537045024718804
36	25.24304057865224	22.688211287700323	25.05391685191944	27.014831281727993
37	24.983133702732893	21.355497306922373	26.157691584547155	27.503677405797582
38	23.867193116338743	21.34554342656801	27.30349381200438	27.483769645088866
39	23.692447216784455	20.75715849895484	28.297775860734152	27.25261842352655
40	23.144983797294756	20.592366479754915	29.350675204884034	26.911974518066295
41	22.546644989327227	20.728402844597806	29.8583231029563	26.866629063118662
42	22.04342103807912	20.45522412820598	30.509749272813742	26.99160556090116
43	20.80913987413871	20.430892420673104	30.870300938982716	27.88966676620547
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	3.0
2	3.0
3	4.0
4	5.0
5	5.0
6	4.5
7	4.0
8	4.0
9	4.0
10	6.0
11	8.0
12	8.0
13	20.0
14	32.0
15	53.0
16	74.0
17	82.5
18	91.0
19	91.0
20	134.0
21	177.0
22	213.5
23	250.0
24	328.5
25	407.0
26	407.0
27	571.5
28	736.0
29	909.5
30	1083.0
31	1352.5
32	1622.0
33	1622.0
34	1908.5
35	2195.0
36	2618.5
37	3042.0
38	3403.5
39	3765.0
40	3765.0
41	4161.0
42	4557.0
43	4832.0
44	5107.0
45	5486.0
46	5865.0
47	5865.0
48	6209.5
49	6554.0
50	6789.5
51	7025.0
52	7345.0
53	7665.0
54	7665.0
55	7647.5
56	7630.0
57	7423.0
58	7216.0
59	6803.0
60	6390.0
61	6390.0
62	5917.5
63	5445.0
64	4918.5
65	4392.0
66	3759.5
67	3127.0
68	3127.0
69	2710.5
70	2294.0
71	1950.5
72	1607.0
73	1231.0
74	855.0
75	855.0
76	676.5
77	498.0
78	411.5
79	325.0
80	258.5
81	192.0
82	192.0
83	143.5
84	95.0
85	68.5
86	42.0
87	30.0
88	18.0
89	18.0
90	17.0
91	16.0
92	8.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	90417.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.19617992191733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.36378567029358	80.47601667827952
2	3.928864709505235	6.773062587787695
3	1.0842229521658797	2.8036762998108764
4	0.5312050913570109	1.8315139852018978
5	0.3515705194005338	1.515201787274517
6	0.1822007801272839	0.9423006735459041
7	0.13600903305276124	0.8206421358815268
8	0.1141962636009033	0.7874625347003329
9	0.06030589201396017	0.4678323766548326
>10	0.24635598439745432	3.4506785228441554
>50	0.0	0.0
>100	0.0012831040854034078	0.1316124180187354
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	119	0.1316124180187354	TruSeq Adapter, Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0011059867060397935	0.0	0.0
2	0.0	0.0	0.0011059867060397935	0.0	0.0
3	0.0	0.0	0.0011059867060397935	0.0	0.0
4	0.0	0.0	0.0011059867060397935	0.0	0.0
5	0.0	0.0	0.0011059867060397935	0.0	0.0
6	0.0	0.0	0.0011059867060397935	0.0	0.0
7	0.0	0.0	0.0011059867060397935	0.0	0.0
8	0.0	0.0	0.0011059867060397935	0.0	0.0
9	0.0	0.0	0.0011059867060397935	0.0	0.0
10	0.0	0.0	0.0011059867060397935	0.0	0.0
11	0.0	0.0	0.0011059867060397935	0.0	0.0
12	0.0	0.0	0.0011059867060397935	0.0	0.0
13	0.0	0.0	0.0011059867060397935	0.0	0.0
14	0.0	0.0	0.0011059867060397935	0.0	0.0
15	0.0	0.0	0.0011059867060397935	0.0	0.0
16	0.0	0.0	0.0011059867060397935	0.0	0.0
17	0.0	0.0	0.0011059867060397935	0.0	0.0
18	0.0	0.0	0.0011059867060397935	0.0	0.0
19	0.0	0.0	0.0011059867060397935	0.0	0.0
20	0.0	0.0	0.0011059867060397935	0.0	0.0
21	0.0	0.0	0.0011059867060397935	0.0	0.0
22	0.0	0.0	0.0011059867060397935	0.00331796011811938	0.0
23	0.0	0.0	0.0011059867060397935	0.005529933530198967	0.0
24	0.0	0.0	0.0011059867060397935	0.005529933530198967	0.0
25	0.0	0.0	0.0011059867060397935	0.005529933530198967	0.0
26	0.0	0.0	0.0011059867060397935	0.011059867060397934	0.0
27	0.0	0.0	0.0011059867060397935	0.04092150812347235	0.0
28	0.0	0.0	0.0011059867060397935	0.11944656425229769	0.0
29	0.0	0.0	0.0011059867060397935	0.2643308227435106	0.0
30	0.0	0.0	0.0011059867060397935	0.4479246159461163	0.0
31	0.0	0.0	0.0011059867060397935	0.942300673545904	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCCG	35	8.806073E-4	26.428572	2
CCTATTA	35	8.806073E-4	26.428572	2
TTGGACA	40	0.0019175305	23.125	4
TCTCGTA	40	0.0019175305	23.125	30
TCGTATG	40	0.0019175305	23.125	32
GTATCAA	40	0.0019175305	23.125	1
ATCTCGT	45	0.0037990105	20.555557	29
CTCGTAT	45	0.0037990105	20.555557	31
TATGCCG	45	0.0037990105	20.555557	35
CCCACGA	45	0.0037990105	20.555557	11
GTATGCC	45	0.0037990105	20.555557	34
ATGCCGT	45	0.0037990105	20.555557	36
TATACAC	45	0.0037990105	20.555557	37
TGCCGTC	45	0.0037990105	20.555557	37
TATCAAC	105	2.1913365E-8	19.38095	1
ATCAACG	115	2.9413059E-9	19.304348	2
CAACGCA	110	3.7362042E-8	18.5	4
CTTGGAC	60	9.1473944E-4	18.5	3
TCAACGC	110	3.7362042E-8	18.5	3
CGTATGC	50	0.006986081	18.499998	33
>>END_MODULE