FastQCFastQC Report
Fri 10 Feb 2017
ERR1632101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429539
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG250.00549255929.5999985
TAGTACG250.00549255929.5999984
GGTCTAT358.860316E-426.428571
ATAGGAC451.3213279E-424.6666663
CATAACC502.698286E-422.1999993
GGTATCA1301.8189894E-1221.3461531
CTATGCT450.003821965820.5555554
ATTAATA450.003821965820.5555552
ATACCGC450.003821965820.55555527
TAGAACT759.243629E-619.7333344
GTTCAAA701.21694255E-418.51
GACACCG500.00702788618.4999987
ATAGTAC500.00702788618.4999983
CCCGACT500.00702788618.49999831
CTTATAC5400.017.47222137
TTAATAC650.001577757117.0769233
TGTATTG650.001577757117.0769235
TACACAA803.376398E-416.18755
TAATACA700.002588950615.8571424
ATACTTT700.002588950615.8571426