##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632101.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	429539
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83375199923639	34.0	31.0	34.0	31.0	34.0
2	32.9990943779261	34.0	33.0	34.0	31.0	34.0
3	33.08587811584047	34.0	33.0	34.0	31.0	34.0
4	36.456694269903316	37.0	37.0	37.0	35.0	37.0
5	36.32644532859647	37.0	37.0	37.0	35.0	37.0
6	36.355616137300686	37.0	37.0	37.0	35.0	37.0
7	36.33643278025977	37.0	37.0	37.0	35.0	37.0
8	36.32206621517487	37.0	37.0	37.0	35.0	37.0
9	38.13027455015726	39.0	39.0	39.0	37.0	39.0
10	38.09688060921127	39.0	38.0	39.0	37.0	39.0
11	38.167535427516476	39.0	39.0	39.0	37.0	39.0
12	38.13294019867812	39.0	38.0	39.0	37.0	39.0
13	38.14954404605868	39.0	39.0	39.0	37.0	39.0
14	39.57695575954686	41.0	39.0	41.0	37.0	41.0
15	39.58470592891449	41.0	39.0	41.0	37.0	41.0
16	39.58294590246753	41.0	39.0	41.0	37.0	41.0
17	39.55639418073795	41.0	39.0	41.0	37.0	41.0
18	39.550632189393745	41.0	39.0	41.0	37.0	41.0
19	39.573351895869756	41.0	39.0	41.0	37.0	41.0
20	39.54974053578371	41.0	39.0	41.0	37.0	41.0
21	39.520797878655955	41.0	39.0	41.0	37.0	41.0
22	39.48504792347144	41.0	39.0	41.0	37.0	41.0
23	39.440954139205054	40.0	39.0	41.0	37.0	41.0
24	39.401798206914854	41.0	39.0	41.0	37.0	41.0
25	39.34440877312654	40.0	39.0	41.0	36.0	41.0
26	39.264965462973095	40.0	39.0	41.0	36.0	41.0
27	39.18600406482298	40.0	39.0	41.0	36.0	41.0
28	39.13486086245952	40.0	39.0	41.0	36.0	41.0
29	39.06681116266509	40.0	39.0	41.0	36.0	41.0
30	39.00824837791213	40.0	39.0	41.0	35.0	41.0
31	38.94271532969067	40.0	38.0	41.0	35.0	41.0
32	38.85941439543324	40.0	38.0	41.0	35.0	41.0
33	38.79348790214625	40.0	38.0	41.0	35.0	41.0
34	38.72186227560245	40.0	38.0	41.0	35.0	41.0
35	38.707407243579745	40.0	38.0	41.0	35.0	41.0
36	38.6760503702807	40.0	38.0	41.0	35.0	41.0
37	38.62002984595112	40.0	38.0	41.0	35.0	41.0
38	38.57468821224615	40.0	38.0	41.0	35.0	41.0
39	38.52320743867262	40.0	38.0	41.0	35.0	41.0
40	38.45516472311012	40.0	38.0	41.0	34.0	41.0
41	38.42051362041631	40.0	38.0	41.0	34.0	41.0
42	38.368595168308346	40.0	38.0	41.0	34.0	41.0
43	37.47691129327023	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	1.0
15	1.0
16	2.0
17	2.0
18	2.0
19	6.0
20	24.0
21	31.0
22	65.0
23	129.0
24	220.0
25	330.0
26	504.0
27	780.0
28	1115.0
29	1666.0
30	2283.0
31	3137.0
32	4386.0
33	6296.0
34	9714.0
35	14936.0
36	24062.0
37	44277.0
38	108966.0
39	206602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57439021835037	16.90533339231129	11.750271803026035	27.770004586312304
2	20.494297374627216	19.019460398240906	32.222219635469656	28.264022591662226
3	20.98715134132174	20.09549773128866	27.936229306302803	30.981121621086793
4	16.31958914091619	14.664093365212471	31.68815870037412	37.32815879349721
5	17.771378151925667	32.97139491408231	32.12187950337455	17.135347430617475
6	37.90505635111131	31.86160046002808	14.42546544085636	15.807877748004257
7	32.209648017991384	27.475735614228274	19.48018689804651	20.83442946973383
8	28.99643571363718	31.061440288309093	18.60995159927271	21.33217239878102
9	27.91225011000165	13.446508931668602	17.90896752099344	40.73227343733631
10	18.898167570348676	24.633618833214214	30.89800926109154	25.57020433534557
11	38.983421761469856	19.57936299148622	19.42151934981457	22.015695897229357
12	24.06789604669192	22.811199914326753	26.907684750395187	26.21321928858614
13	32.91156332719497	17.560919963030134	21.472788268352815	28.054728441422082
14	24.738615119930905	18.468637306507674	22.432887351323163	34.35986022223826
15	27.719252500937053	24.892268222443132	19.932066704071108	27.45641257254871
16	27.8938582992464	23.316625498499555	21.572662784985763	27.216853417268283
17	26.521456724534907	23.69912860066257	22.86660815432359	26.91280652047893
18	26.673247365198506	22.867073769785748	23.376457085386892	27.083221779628857
19	27.26783831037461	22.916661816505602	23.11384996472963	26.701649908390156
20	27.52159873725087	22.790247218529633	22.948323667932367	26.739830376287138
21	27.822619133536186	22.84030088071165	22.351870260907624	26.985209724844545
22	28.051003517724816	22.658943658201004	22.1111470669718	27.178905757102385
23	27.407522949022088	22.789781603067475	22.329986334186184	27.472709113724246
24	27.40240117893835	22.83168699466172	22.195889081084605	27.570022745315327
25	27.566763437080215	22.28552005755007	22.483639436698414	27.664077068671293
26	27.144683020633746	22.641715886101146	22.73204528575985	27.481555807505252
27	26.948193295602962	22.912471277346178	22.76417275264877	27.37516267440209
28	27.15515936853231	22.67826669988057	22.85077722861021	27.315796702976915
29	26.780804536957064	22.986504135829342	23.10314080909999	27.129550518113604
30	26.786159114771884	23.232814715311065	22.68664777819942	27.294378391717633
31	27.11185713055159	22.710859782231648	22.55720667971942	27.62007640749734
32	26.549626459995483	22.89035454289366	23.090801999352795	27.46921699775806
33	26.48723398806628	22.543471023585752	23.529877380168042	27.439417608179934
34	26.932827985351736	22.002658664288923	23.61601624066732	27.448497109692017
35	26.418090091935774	22.03874386260619	24.2934867381076	27.249679307350437
36	26.122889888927432	21.868328603456263	24.16171756231681	27.847063945299492
37	25.784853063400533	21.289335776262458	24.968163542774928	27.95764761756208
38	25.259638822086007	21.14313252114476	25.961554131289592	27.635674525479644
39	24.94162346143191	20.871678706706494	26.63669655141908	27.550001280442522
40	24.416409220117384	20.677517058986496	27.51205362027662	27.394020100619503
41	23.807849811076526	20.69544325427959	28.031913283776326	27.46479365086756
42	23.528014918319407	20.984357648548794	28.308721676029418	27.178905757102385
43	22.80980306794028	20.47427590975441	28.623244920717326	28.092676101587983
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	5.0
2	5.0
3	18.5
4	32.0
5	32.0
6	40.0
7	48.0
8	48.5
9	49.0
10	55.5
11	62.0
12	62.0
13	130.0
14	198.0
15	268.0
16	338.0
17	406.0
18	474.0
19	474.0
20	621.0
21	768.0
22	954.0
23	1140.0
24	1524.5
25	1909.0
26	1909.0
27	2437.0
28	2965.0
29	3695.0
30	4425.0
31	5497.5
32	6570.0
33	6570.0
34	7919.5
35	9269.0
36	10761.0
37	12253.0
38	13839.0
39	15425.0
40	15425.0
41	17142.0
42	18859.0
43	20403.0
44	21947.0
45	23713.0
46	25479.0
47	25479.0
48	27064.0
49	28649.0
50	30222.5
51	31796.0
52	32726.5
53	33657.0
54	33657.0
55	33960.5
56	34264.0
57	34096.0
58	33928.0
59	33200.0
60	32472.0
61	32472.0
62	30798.5
63	29125.0
64	26442.5
65	23760.0
66	21154.5
67	18549.0
68	18549.0
69	16120.5
70	13692.0
71	11857.0
72	10022.0
73	8278.5
74	6535.0
75	6535.0
76	5525.0
77	4515.0
78	3628.0
79	2741.0
80	2279.0
81	1817.0
82	1817.0
83	1370.0
84	923.0
85	724.5
86	526.0
87	367.5
88	209.0
89	209.0
90	159.0
91	109.0
92	65.0
93	21.0
94	13.5
95	6.0
96	6.0
97	4.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	429539.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.38731470755313
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.14935193472846	76.00699695170718
2	5.7022251799396395	9.50986491225921
3	1.4129635832679386	3.534697169648266
4	0.6064200001988127	2.0227094160613133
5	0.31137682516464116	1.298243865632134
6	0.20128965939754795	1.0071002505335707
7	0.13321258349769208	0.7775767736189773
8	0.091360090200283	0.6094618074593151
9	0.07573728842474182	0.56839761944736
>10	0.30936638508170144	4.193102684911314
>50	0.005022352657093653	0.27105698820203666
>100	0.0016741174414888868	0.20079156051928745
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.3280773107913367E-4	0.0
4	0.0	0.0	0.0	2.3280773107913367E-4	0.0
5	0.0	0.0	0.0	2.3280773107913367E-4	0.0
6	0.0	0.0	0.0	2.3280773107913367E-4	0.0
7	0.0	0.0	0.0	2.3280773107913367E-4	0.0
8	0.0	0.0	0.0	2.3280773107913367E-4	0.0
9	0.0	2.3280773107913367E-4	0.0	4.6561546215826734E-4	0.0
10	0.0	2.3280773107913367E-4	0.0	4.6561546215826734E-4	0.0
11	0.0	2.3280773107913367E-4	0.0	4.6561546215826734E-4	0.0
12	0.0	2.3280773107913367E-4	0.0	6.98423193237401E-4	0.0
13	0.0	2.3280773107913367E-4	0.0	6.98423193237401E-4	0.0
14	0.0	2.3280773107913367E-4	0.0	6.98423193237401E-4	0.0
15	0.0	2.3280773107913367E-4	0.0	6.98423193237401E-4	0.0
16	0.0	2.3280773107913367E-4	0.0	9.312309243165347E-4	0.0
17	0.0	2.3280773107913367E-4	0.0	9.312309243165347E-4	0.0
18	0.0	2.3280773107913367E-4	0.0	0.0011640386553956683	0.0
19	0.0	2.3280773107913367E-4	0.0	0.001396846386474802	0.0
20	0.0	2.3280773107913367E-4	0.0	0.0016296541175539357	0.0
21	0.0	2.3280773107913367E-4	0.0	0.002095269579712203	0.0
22	0.0	2.3280773107913367E-4	0.0	0.002095269579712203	0.0
23	0.0	2.3280773107913367E-4	0.0	0.0032593082351078714	0.0
24	0.0	2.3280773107913367E-4	0.0	0.005121770083740941	0.0
25	0.0	2.3280773107913367E-4	0.0	0.007217039663453144	0.0
26	0.0	2.3280773107913367E-4	0.0	0.010709155629640149	0.0
27	0.0	2.3280773107913367E-4	0.0	0.041905391594244064	0.0
28	0.0	2.3280773107913367E-4	0.0	0.12920829074891918	0.0
29	0.0	2.3280773107913367E-4	0.0	0.26726327527884547	0.0
30	0.0	2.3280773107913367E-4	0.0	0.4604936920745264	0.0
31	0.0	2.3280773107913367E-4	0.0	0.9594006597771099	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTCCG	25	0.005492559	29.599998	5
TAGTACG	25	0.005492559	29.599998	4
GGTCTAT	35	8.860316E-4	26.42857	1
ATAGGAC	45	1.3213279E-4	24.666666	3
CATAACC	50	2.698286E-4	22.199999	3
GGTATCA	130	1.8189894E-12	21.346153	1
CTATGCT	45	0.0038219658	20.555555	4
ATTAATA	45	0.0038219658	20.555555	2
ATACCGC	45	0.0038219658	20.555555	27
TAGAACT	75	9.243629E-6	19.733334	4
GTTCAAA	70	1.21694255E-4	18.5	1
GACACCG	50	0.007027886	18.499998	7
ATAGTAC	50	0.007027886	18.499998	3
CCCGACT	50	0.007027886	18.499998	31
CTTATAC	540	0.0	17.472221	37
TTAATAC	65	0.0015777571	17.076923	3
TGTATTG	65	0.0015777571	17.076923	5
TACACAA	80	3.376398E-4	16.1875	5
TAATACA	70	0.0025889506	15.857142	4
ATACTTT	70	0.0025889506	15.857142	6
>>END_MODULE