FastQCFastQC Report
Fri 10 Feb 2017
ERR1632100.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632100.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107303
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGGT250.00547443729.63
CTTATAC754.5110937E-1027.13333337
GTCCCTT358.816875E-426.4285728
CCTACTC400.001919860523.1253
ACTCCTA400.001919860523.1256
AGGTCCC400.001919860523.1256
GGTATCA603.6859237E-521.5833321
TACTCCT450.003803582420.5555575
GTATTTT500.006994406718.53
CTACTCC500.006994406718.54
GCATGCA500.006994406718.52
TATCAAC956.964818E-515.5789483
GTATCAA855.308085E-415.2352942
TCTCTTA2850.014.92982437
TCTTATA1458.69446E-714.03448336
ATCAACG950.001232247513.6315794
AGCCTGT850.00933226213.05882436
CAACGCA1000.001813740712.9500016
TCAACGC1050.002616729412.3333345
CTCTTAT1653.7130285E-612.33333335