FastQCFastQC Report
Fri 10 Feb 2017
ERR1632099.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632099.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1187570
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.1802841095682781No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19760.1663901917360661No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15300.12883451080778396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.027.2392641
CGATTAA358.869572E-426.4285724
CGCGGTT1551.8189894E-1219.09677310
GACGGAC2250.018.9111127
CAAGACG2550.018.8627454
CTTATAC12850.018.85992237
TAAACCG609.237062E-418.514
CGTATAT500.00703501818.4999982
GTATCAA12500.017.6122
ACGGACC2450.017.3673488
CTTATAG752.0673385E-417.2666663
ATAGGAC2150.017.2093033
CGGTTCT1655.4569682E-1116.81818212
GCGGTTC1801.0913936E-1116.44444511
CGGACCA2700.016.4444459
TCTTATA21550.016.1392137
TAGGGGT1151.2422024E-616.0869564
AGTACGG1403.4728146E-815.85714236
AAGACGG2850.015.5789475
CGCAAGA2750.015.4727282
Produced by FastQC (version 0.11.5)