##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632099.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1187570
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.86255462835875	34.0	31.0	34.0	31.0	34.0
2	33.021365477403435	34.0	33.0	34.0	31.0	34.0
3	33.10459762371902	34.0	33.0	34.0	31.0	34.0
4	36.47154609833525	37.0	37.0	37.0	35.0	37.0
5	36.355912493579325	37.0	37.0	37.0	35.0	37.0
6	36.389279789822915	37.0	37.0	37.0	35.0	37.0
7	36.36781242368871	37.0	37.0	37.0	35.0	37.0
8	36.35724630969122	37.0	37.0	37.0	35.0	37.0
9	38.160291182835536	39.0	39.0	39.0	37.0	39.0
10	38.13571073705129	39.0	38.0	39.0	37.0	39.0
11	38.20287477790783	39.0	39.0	39.0	37.0	39.0
12	38.171745665518664	39.0	39.0	39.0	37.0	39.0
13	38.18313699402982	39.0	39.0	39.0	37.0	39.0
14	39.60269036772569	41.0	39.0	41.0	37.0	41.0
15	39.624063423629764	41.0	39.0	41.0	37.0	41.0
16	39.61824145103026	41.0	39.0	41.0	37.0	41.0
17	39.61448756704868	41.0	39.0	41.0	37.0	41.0
18	39.611992556228266	41.0	39.0	41.0	37.0	41.0
19	39.64386688784661	41.0	39.0	41.0	37.0	41.0
20	39.624776644745154	41.0	39.0	41.0	37.0	41.0
21	39.59944845356484	41.0	39.0	41.0	37.0	41.0
22	39.56370908662226	41.0	39.0	41.0	37.0	41.0
23	39.51451451282872	41.0	39.0	41.0	37.0	41.0
24	39.47458339297894	41.0	39.0	41.0	37.0	41.0
25	39.4279124598971	41.0	39.0	41.0	37.0	41.0
26	39.3576892309506	40.0	39.0	41.0	36.0	41.0
27	39.28272522882862	40.0	39.0	41.0	36.0	41.0
28	39.21959210825467	40.0	39.0	41.0	36.0	41.0
29	39.16088904233013	40.0	39.0	41.0	36.0	41.0
30	39.09834704480578	40.0	39.0	41.0	36.0	41.0
31	39.03273575452394	40.0	39.0	41.0	35.0	41.0
32	38.95742819370648	40.0	39.0	41.0	35.0	41.0
33	38.89215119950824	40.0	38.0	41.0	35.0	41.0
34	38.820943607534716	40.0	38.0	41.0	35.0	41.0
35	38.798341992472025	40.0	38.0	41.0	35.0	41.0
36	38.75758902633108	40.0	38.0	41.0	35.0	41.0
37	38.710888621302324	40.0	38.0	41.0	35.0	41.0
38	38.66736781831808	40.0	38.0	41.0	35.0	41.0
39	38.61865237417584	40.0	38.0	41.0	35.0	41.0
40	38.55823319888512	40.0	38.0	41.0	35.0	41.0
41	38.51156479197016	40.0	38.0	41.0	35.0	41.0
42	38.46751938833079	40.0	38.0	41.0	35.0	41.0
43	37.63919684734373	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	1.0
13	0.0
14	0.0
15	1.0
16	2.0
17	7.0
18	20.0
19	34.0
20	75.0
21	140.0
22	237.0
23	352.0
24	611.0
25	971.0
26	1358.0
27	2032.0
28	2994.0
29	4312.0
30	6049.0
31	8343.0
32	11468.0
33	16712.0
34	24790.0
35	37607.0
36	61850.0
37	116117.0
38	288812.0
39	602671.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.143199979790666	18.614986906035014	12.919827883830006	26.321985230344318
2	18.900191146627147	20.529905605564302	34.49489293262713	26.07501031518142
3	19.881017540018693	21.674174996000236	30.32250730483256	28.122300159148512
4	15.237501789368205	15.743829837399058	34.03546738297532	34.983200990257416
5	15.746187593152403	34.99768434702796	33.64711132817434	15.609016731645292
6	34.70481740023746	34.22897176587485	15.756629082917218	15.30958175097047
7	29.437254225014104	29.850703537475688	21.016192729691724	19.695849507818487
8	27.04766876899888	32.79040393408388	20.2807413457733	19.88118595114393
9	26.772400784795842	14.335239185900619	19.976927675842266	38.91543235346127
10	17.422888756031224	26.161236811303755	32.824844009195246	23.591030423469775
11	36.06482144210447	21.283966418821628	21.270409323239896	21.380802815834013
12	22.367186776358448	25.045007873220104	28.437397374470557	24.15040797595089
13	30.935692211827515	19.211583317193934	24.184848051062254	25.667876419916304
14	23.526023729127544	20.75431342994518	24.346017497915913	31.373645343011358
15	25.985668213242167	27.04935288025127	21.87104760140455	25.093931305102014
16	25.579292168040617	25.71031602347651	23.499246360214556	25.21114544826831
17	24.233771482944164	25.836287545155233	25.050144412539893	24.879796559360713
18	24.162617782530717	24.525459551857995	26.22354892764216	25.088373737969132
19	24.978401273188105	24.912131495406587	26.019434643852573	24.090032587552734
20	25.65204577414384	24.246991756275417	25.64034120093973	24.460621268641006
21	25.845297540355517	24.885690948744074	24.605539041909108	24.6634724689913
22	25.947186271125073	24.57834064518302	24.709280295056292	24.765192788635616
23	25.159190616132104	24.559141776905783	24.94320334801317	25.338464258948946
24	25.14975959311872	24.858745168705845	25.05006020697727	24.94143503119816
25	25.30116119470852	24.229982232626288	25.092668221662723	25.376188351002465
26	24.893185243817207	24.921730929545205	25.03793460596007	25.147149220677516
27	25.140244364542724	24.540953375379978	24.973769967244035	25.345032292833263
28	24.617496231801073	24.787591468292394	25.445657940163528	25.149254359743008
29	24.2815160369494	25.029093021885025	25.71216854585414	24.977222395311436
30	24.396204013237114	25.534494808727064	25.408775903736203	24.66052527429962
31	24.98143267344241	25.0337243278291	24.99364248002223	24.991200518706265
32	24.013994964507354	24.685702737522842	25.611795515211732	25.68850678275807
33	23.997069646420844	24.476872942226564	26.05606406359204	25.469993347760557
34	24.797106696868397	23.86158289616612	26.031560244869777	25.309750162095707
35	24.409845314381467	23.911769411487324	26.610810310129086	25.06757496400212
36	23.93745210808626	24.020310381703815	26.574686123765336	25.467551386444587
37	24.257096423789758	22.886987714408413	27.006071221064865	25.84984464073697
38	23.323930378840828	22.9868555116751	28.193453859561963	25.49576024992211
39	23.15897168166929	22.59504702880672	28.756957484611434	25.489023804912552
40	22.580311055348314	22.50132623761126	29.565246680195695	25.353116026844734
41	21.890414880807025	22.54544995242386	30.22255530200325	25.34157986476587
42	22.04493208821375	22.498210631794336	30.018441018213665	25.43841626177825
43	21.047432993423545	22.280707663548256	30.4416581759391	26.230201167089096
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	103.0
1	112.0
2	121.0
3	243.0
4	365.0
5	365.0
6	479.5
7	594.0
8	582.0
9	570.0
10	834.0
11	1098.0
12	1098.0
13	1845.5
14	2593.0
15	3886.5
16	5180.0
17	5220.0
18	5260.0
19	5260.0
20	6464.0
21	7668.0
22	8284.5
23	8901.0
24	10905.5
25	12910.0
26	12910.0
27	15114.0
28	17318.0
29	21534.5
30	25751.0
31	29225.0
32	32699.0
33	32699.0
34	36913.0
35	41127.0
36	45683.5
37	50240.0
38	54547.5
39	58855.0
40	58855.0
41	63257.5
42	67660.0
43	69148.5
44	70637.0
45	73534.5
46	76432.0
47	76432.0
48	78182.0
49	79932.0
50	82668.0
51	85404.0
52	89927.5
53	94451.0
54	94451.0
55	90858.5
56	87266.0
57	83845.0
58	80424.0
59	74804.0
60	69184.0
61	69184.0
62	63736.0
63	58288.0
64	51902.5
65	45517.0
66	39474.5
67	33432.0
68	33432.0
69	28559.0
70	23686.0
71	20538.5
72	17391.0
73	13929.0
74	10467.0
75	10467.0
76	8287.5
77	6108.0
78	5140.5
79	4173.0
80	3404.0
81	2635.0
82	2635.0
83	2012.0
84	1389.0
85	1140.0
86	891.0
87	710.5
88	530.0
89	530.0
90	395.5
91	261.0
92	152.5
93	44.0
94	27.0
95	10.0
96	10.0
97	7.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1187570.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.98874754913922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.69880084354507	60.98509126012301
2	6.234128077997423	8.477011201679346
3	1.49026805675441	3.039643760736656
4	0.6741758059402406	1.8334547469523395
5	0.39128206729302245	1.3301388846845306
6	0.2423344636958208	0.9885610004802724
7	0.18452751186720448	0.878205609414711
8	0.12752714502256995	0.6936328694881579
9	0.10920307059879503	0.6682121998679073
>10	0.6979099025984233	9.534566454505335
>50	0.0933638004806109	4.472458604038423
>100	0.05561225014089513	6.42467521308444
>500	4.954308946032707E-4	0.19881819284028632
>1k	3.715731709524531E-4	0.47553000210465773
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2141	0.1802841095682781	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1976	0.1663901917360661	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1530	0.12883451080778396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	8.420556261946664E-5	0.0
16	0.0	8.420556261946664E-5	0.0	4.210278130973332E-4	0.0
17	8.420556261946664E-5	8.420556261946664E-5	0.0	6.736445009557331E-4	0.0
18	8.420556261946664E-5	1.6841112523893328E-4	0.0	7.578500635751998E-4	0.0
19	8.420556261946664E-5	1.6841112523893328E-4	0.0	0.0012630834392919996	0.0
20	8.420556261946664E-5	1.6841112523893328E-4	0.0	0.001431494564530933	0.0
21	8.420556261946664E-5	1.6841112523893328E-4	0.0	0.0016841112523893328	0.0
22	8.420556261946664E-5	1.6841112523893328E-4	0.0	0.0024419613159645326	0.0
23	8.420556261946664E-5	1.6841112523893328E-4	0.0	0.003957661443114932	0.0
24	8.420556261946664E-5	1.6841112523893328E-4	0.0	0.006231211633840532	0.0
25	8.420556261946664E-5	1.6841112523893328E-4	0.0	0.008673172949805064	0.0
26	8.420556261946664E-5	1.6841112523893328E-4	0.0	0.013893917832211996	0.0
27	8.420556261946664E-5	1.6841112523893328E-4	0.0	0.037808297616140525	0.0
28	8.420556261946664E-5	2.526166878583999E-4	0.0	0.12049816010845676	0.0
29	8.420556261946664E-5	2.526166878583999E-4	0.0	0.24924846535362127	0.0
30	8.420556261946664E-5	2.526166878583999E-4	0.0	0.431637713987386	0.0
31	8.420556261946664E-5	2.526166878583999E-4	0.0	0.9661746254957603	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	815	0.0	27.239264	1
CGATTAA	35	8.869572E-4	26.42857	24
CGCGGTT	155	1.8189894E-12	19.096773	10
GACGGAC	225	0.0	18.911112	7
CAAGACG	255	0.0	18.862745	4
CTTATAC	1285	0.0	18.859922	37
TAAACCG	60	9.237062E-4	18.5	14
CGTATAT	50	0.007035018	18.499998	2
GTATCAA	1250	0.0	17.612	2
ACGGACC	245	0.0	17.367348	8
CTTATAG	75	2.0673385E-4	17.266666	3
ATAGGAC	215	0.0	17.209303	3
CGGTTCT	165	5.4569682E-11	16.818182	12
GCGGTTC	180	1.0913936E-11	16.444445	11
CGGACCA	270	0.0	16.444445	9
TCTTATA	2155	0.0	16.13921	37
TAGGGGT	115	1.2422024E-6	16.086956	4
AGTACGG	140	3.4728146E-8	15.857142	36
AAGACGG	285	0.0	15.578947	5
CGCAAGA	275	0.0	15.472728	2
>>END_MODULE