FastQCFastQC Report
Fri 10 Feb 2017
ERR1632098.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632098.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634140
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA24220.1482125154515525No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC23990.14680504730316862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.1351169422448505No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG18210.11143476079160904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17330.10604966526735775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10400.024.3701921
CGAATTA450.003826487820.55555715
TGTTACG450.003826487820.55555716
CTTATAC21200.019.45990437
CGACGGT1502.5102054E-1017.2666667
TACGACG1655.4569682E-1116.8181825
ATACCGC2350.016.53191627
ACGACGG1606.2937033E-1016.18756
TACCGTC1659.749783E-1015.696977
ACGGTAT1659.749783E-1015.696979
GTAAACG2055.456968E-1215.34146327
GTATCAA16750.015.2417912
CTATACA855.365869E-415.2352944
TAACCTA855.365869E-415.2352945
TCTTATA34600.014.91763137
GACGGTA1752.239176E-914.7999998
ATACCGT1752.239176E-914.7999996
TCGCCAT7150.014.74825113
CAAGACG2400.014.6458334
AGTGTGT1304.446436E-614.2307698
Produced by FastQC (version 0.11.5)