##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632098.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1634140
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.787365219626224	34.0	31.0	34.0	31.0	34.0
2	32.953153952537726	34.0	31.0	34.0	31.0	34.0
3	33.036086259439216	34.0	33.0	34.0	31.0	34.0
4	36.4277399733193	37.0	37.0	37.0	35.0	37.0
5	36.30315946002179	37.0	37.0	37.0	35.0	37.0
6	36.331294748307975	37.0	37.0	37.0	35.0	37.0
7	36.31083260920117	37.0	37.0	37.0	35.0	37.0
8	36.29486886068513	37.0	37.0	37.0	35.0	37.0
9	38.081712705153784	39.0	38.0	39.0	37.0	39.0
10	38.058354241374666	39.0	38.0	39.0	35.0	39.0
11	38.12485711138581	39.0	38.0	39.0	37.0	39.0
12	38.09551935574675	39.0	38.0	39.0	37.0	39.0
13	38.10727416255645	39.0	38.0	39.0	37.0	39.0
14	39.51432496603718	40.0	39.0	41.0	37.0	41.0
15	39.53786884844628	40.0	39.0	41.0	37.0	41.0
16	39.52550393479139	41.0	39.0	41.0	37.0	41.0
17	39.52008334659209	41.0	39.0	41.0	37.0	41.0
18	39.50899739312421	41.0	39.0	41.0	37.0	41.0
19	39.54438420208795	41.0	39.0	41.0	37.0	41.0
20	39.52092293193974	41.0	39.0	41.0	37.0	41.0
21	39.49070397885126	41.0	39.0	41.0	37.0	41.0
22	39.46089196764047	40.0	39.0	41.0	37.0	41.0
23	39.411757254580394	40.0	39.0	41.0	37.0	41.0
24	39.371782711395596	41.0	39.0	41.0	37.0	41.0
25	39.31612285361108	40.0	39.0	41.0	36.0	41.0
26	39.23863928427185	40.0	39.0	41.0	36.0	41.0
27	39.16548398546024	40.0	39.0	41.0	36.0	41.0
28	39.09555240065111	40.0	39.0	41.0	36.0	41.0
29	39.04147135496347	40.0	39.0	41.0	35.0	41.0
30	38.97389024196213	40.0	39.0	41.0	35.0	41.0
31	38.91504032702216	40.0	38.0	41.0	35.0	41.0
32	38.828082049273625	40.0	38.0	41.0	35.0	41.0
33	38.76015947226064	40.0	38.0	41.0	35.0	41.0
34	38.68746190656859	40.0	38.0	41.0	35.0	41.0
35	38.66605248020365	40.0	38.0	41.0	35.0	41.0
36	38.62844737904953	40.0	38.0	41.0	35.0	41.0
37	38.58684445641132	40.0	38.0	41.0	35.0	41.0
38	38.53890609127737	40.0	38.0	41.0	35.0	41.0
39	38.49018382757903	40.0	38.0	41.0	35.0	41.0
40	38.43683282950053	40.0	38.0	41.0	34.0	41.0
41	38.38986684127431	40.0	38.0	41.0	34.0	41.0
42	38.35323289314257	40.0	38.0	41.0	34.0	41.0
43	37.48591491549072	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	2.0
15	2.0
16	3.0
17	11.0
18	30.0
19	58.0
20	112.0
21	197.0
22	356.0
23	599.0
24	971.0
25	1508.0
26	2225.0
27	3141.0
28	4612.0
29	6707.0
30	9424.0
31	13196.0
32	18074.0
33	25329.0
34	37640.0
35	56383.0
36	90870.0
37	167387.0
38	408917.0
39	786383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.351206139008895	17.844737904953064	12.874539513138409	26.929516442899633
2	18.690687456399086	20.29795488758613	34.48321441247384	26.528143243540946
3	19.907780239147197	21.262682511902284	30.168834983538744	28.660702265411775
4	15.533797593841408	15.512440794546368	33.073665659001065	35.88009595261116
5	15.85304808645526	35.02349859865127	33.314159129572744	15.809294185320718
6	35.430807641940106	33.44688949539207	15.688925061500239	15.433377801167586
7	30.03065832792784	29.7286034244312	20.444209186483413	19.79652906115755
8	27.45945879789981	32.88494253858298	19.971422277161075	19.684176386356125
9	26.922723879226997	14.696537628354974	18.952721309067766	39.42801718335026
10	17.77191672684103	26.31671704994676	31.853635551421544	24.057730671790665
11	36.751012765124166	20.889275092709315	21.126464072845653	21.233248069320865
12	22.819525866816797	24.552547517348575	27.67688202969146	24.95104458614317
13	31.07548924816723	18.57943627840944	24.157538521791277	26.18753595163205
14	23.628085720929665	20.005691066860855	23.61670358720795	32.749519625001525
15	26.36940531411017	26.57752701726902	21.397248705741244	25.655818962879557
16	26.35569779823026	25.16045136891576	22.731406121874503	25.75244471097948
17	25.39519257835926	25.316435556317085	23.90040021050828	25.387971654815377
18	24.636444857845717	24.098424859559156	25.482455603559057	25.782674679036067
19	26.012765124163167	24.40341708788721	25.1390333753534	24.444784412596228
20	26.12597451870709	23.742519000820003	25.02717025469054	25.10433622578237
21	26.304600585017194	24.65547627498256	23.733156277919885	25.30676686208036
22	26.511743179898907	23.965694493739825	23.70329347546722	25.81926885089405
23	25.365941718579805	24.41840968338086	24.26156877623704	25.954079821802296
24	26.425948817114815	24.217447709498575	24.049347057167683	25.307256416218927
25	25.994835203838107	23.487950848764488	24.516810065233088	26.00040388216432
26	25.838606239367497	23.916494302813714	24.408006657936284	25.836892799882506
27	25.92556329323069	23.712227838496087	24.16212809184036	26.200080776432866
28	25.02980160818534	24.278152422681043	24.792000685375797	25.900045283757816
29	25.118166130196922	24.743657214192176	24.707185430868837	25.430991224742066
30	24.70687945953223	25.42126133623802	24.403233505085243	25.468625699144503
31	25.693392243014674	24.3405705753485	24.49227116403735	25.47376601759947
32	24.471526307415523	24.236601515170058	24.84266953871761	26.449202638696807
33	24.798120112107895	23.70739349137773	25.255057706194084	26.239428690320292
34	25.393968693012837	23.46585971826159	25.27133538130148	25.86883620742409
35	25.212711273207926	23.30638745762297	25.479518278727646	26.001382990441456
36	24.331452629517667	23.551898858114974	25.943676796357718	26.172971716009645
37	25.211059027990256	21.99279131530958	26.127504375390114	26.66864528131005
38	23.94654068806834	22.224778782723632	27.605407125460484	26.223273403747537
39	23.92102267859547	21.52906115755076	28.2611036998054	26.288812464048366
40	23.420575960444026	21.62360630056176	28.93167048110933	26.024147257884884
41	22.36485246062149	21.947629946026655	29.877427882555963	25.810089710795893
42	22.77142717270246	21.377176986059947	29.554934093774094	26.296461747463496
43	21.27590047364363	21.46584747940813	30.266684616985078	26.99156742996316
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	81.0
1	81.5
2	82.0
3	190.0
4	298.0
5	298.0
6	383.0
7	468.0
8	488.5
9	509.0
10	778.5
11	1048.0
12	1048.0
13	1644.5
14	2241.0
15	3906.0
16	5571.0
17	5572.0
18	5573.0
19	5573.0
20	6197.5
21	6822.0
22	7932.5
23	9043.0
24	10802.5
25	12562.0
26	12562.0
27	14958.5
28	17355.0
29	23246.5
30	29138.0
31	32290.5
32	35443.0
33	35443.0
34	40784.0
35	46125.0
36	51736.5
37	57348.0
38	66788.5
39	76229.0
40	76229.0
41	85690.5
42	95152.0
43	94568.5
44	93985.0
45	101279.5
46	108574.0
47	108574.0
48	114107.0
49	119640.0
50	127285.5
51	134931.0
52	142907.0
53	150883.0
54	150883.0
55	135786.0
56	120689.0
57	114739.0
58	108789.0
59	100414.5
60	92040.0
61	92040.0
62	85872.0
63	79704.0
64	71866.5
65	64029.0
66	56809.0
67	49589.0
68	49589.0
69	42849.0
70	36109.0
71	32062.5
72	28016.0
73	22810.5
74	17605.0
75	17605.0
76	14276.5
77	10948.0
78	9202.5
79	7457.0
80	5989.5
81	4522.0
82	4522.0
83	3515.0
84	2508.0
85	2021.5
86	1535.0
87	1221.5
88	908.0
89	908.0
90	686.5
91	465.0
92	277.0
93	89.0
94	61.0
95	33.0
96	33.0
97	18.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1634140.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.83515606849565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09028896871482	48.626998766185764
2	7.259062201102143	8.106217418189113
3	2.08488856145318	3.49230234642479
4	0.936036689505519	2.0905501857751463
5	0.5532606460374181	1.5445697259027982
6	0.3498630242439646	1.1720793936754583
7	0.2295603116830206	0.8972275080967771
8	0.18749225027255825	0.8374927244481389
9	0.1450723274130009	0.7290122438092325
>10	0.9195246524637943	10.040298840515097
>50	0.1237348638616203	4.822494485309182
>100	0.11057499532426003	12.622143227906948
>500	0.008117500891078875	3.0489094581942213
>1k	0.002523007033713705	1.9697036755673671
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2422	0.1482125154515525	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2399	0.14680504730316862	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2208	0.1351169422448505	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1821	0.11143476079160904	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1733	0.10604966526735775	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.119426732103736E-5	0.0	0.0	0.0
3	0.0	6.119426732103736E-5	0.0	0.0	0.0
4	0.0	6.119426732103736E-5	0.0	0.0	0.0
5	0.0	6.119426732103736E-5	0.0	0.0	0.0
6	0.0	6.119426732103736E-5	0.0	0.0	0.0
7	0.0	6.119426732103736E-5	0.0	0.0	0.0
8	0.0	6.119426732103736E-5	0.0	6.119426732103736E-5	0.0
9	0.0	6.119426732103736E-5	0.0	1.835828019631121E-4	0.0
10	0.0	6.119426732103736E-5	0.0	2.4477706928414944E-4	0.0
11	0.0	6.119426732103736E-5	0.0	2.4477706928414944E-4	0.0
12	0.0	6.119426732103736E-5	0.0	3.059713366051868E-4	0.0
13	0.0	6.119426732103736E-5	0.0	3.059713366051868E-4	0.0
14	0.0	6.119426732103736E-5	0.0	3.671656039262242E-4	0.0
15	0.0	6.119426732103736E-5	0.0	3.671656039262242E-4	0.0
16	0.0	6.119426732103736E-5	0.0	5.507484058893362E-4	0.0
17	0.0	6.119426732103736E-5	0.0	6.731369405314111E-4	0.0
18	0.0	6.119426732103736E-5	0.0	8.567197424945231E-4	0.0
19	0.0	6.119426732103736E-5	0.0	0.0010403025444576351	0.0
20	0.0	6.119426732103736E-5	0.0	0.0014074681483838595	0.0
21	0.0	6.119426732103736E-5	0.0	0.001652245217668009	0.0
22	0.0	6.119426732103736E-5	0.0	0.0024477706928414944	0.0
23	0.0	6.119426732103736E-5	0.0	0.0032432961680149805	0.0
24	0.0	6.119426732103736E-5	0.0	0.0052015127222881765	0.0
25	0.0	6.119426732103736E-5	0.0	0.00697614647459826	0.0
26	0.0	6.119426732103736E-5	0.0	0.011259745187070876	0.0
27	0.0	6.119426732103736E-5	0.0	0.03806283427368524	0.0
28	0.0	6.119426732103736E-5	0.0	0.14013487216517556	0.0
29	0.0	1.2238853464207472E-4	0.0	0.28339065196372404	0.0
30	0.0	1.2238853464207472E-4	0.0	0.48098694114335366	0.0
31	0.0	1.2238853464207472E-4	0.0	1.102231142986525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1040	0.0	24.370192	1
CGAATTA	45	0.0038264878	20.555557	15
TGTTACG	45	0.0038264878	20.555557	16
CTTATAC	2120	0.0	19.459904	37
CGACGGT	150	2.5102054E-10	17.266666	7
TACGACG	165	5.4569682E-11	16.818182	5
ATACCGC	235	0.0	16.531916	27
ACGACGG	160	6.2937033E-10	16.1875	6
TACCGTC	165	9.749783E-10	15.69697	7
ACGGTAT	165	9.749783E-10	15.69697	9
GTAAACG	205	5.456968E-12	15.341463	27
GTATCAA	1675	0.0	15.241791	2
CTATACA	85	5.365869E-4	15.235294	4
TAACCTA	85	5.365869E-4	15.235294	5
TCTTATA	3460	0.0	14.917631	37
GACGGTA	175	2.239176E-9	14.799999	8
ATACCGT	175	2.239176E-9	14.799999	6
TCGCCAT	715	0.0	14.748251	13
CAAGACG	240	0.0	14.645833	4
AGTGTGT	130	4.446436E-6	14.230769	8
>>END_MODULE