FastQCFastQC Report
Fri 10 Feb 2017
ERR1632097.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632097.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences918344
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16950.18457135888076798No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15250.1660597771641128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12370.13469897990295576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA250.005495774629.620
TTACGTT502.7015284E-422.218
GGTATCA5650.021.610621
TAGACTG957.1395334E-921.4210535
CTTATAC10500.020.96666737
ATAGACG500.0070338318.53
TCTATAG852.7221511E-517.4117643
TACCGTC752.0666963E-417.2666667
TACGCTA650.001579606817.07692326
ATATACG650.001579606817.07692323
AATTACG650.001579606817.07692316
ATACGCT650.001579606817.07692325
CGTCATA650.001579606817.07692328
CCTAGAC904.4443907E-516.4444453
TCTTATA19100.016.17539237
ACGTTCG700.002591962215.8571427
CGGTGCG700.002591962215.8571429
ATTTATA1508.093593E-814.82
TATACGC750.004103570714.824
TAAGCTA750.004103570714.85
Produced by FastQC (version 0.11.5)