##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632096.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117307
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72631641760509	34.0	31.0	34.0	31.0	34.0
2	32.89537708747134	34.0	31.0	34.0	31.0	34.0
3	32.98476646747424	34.0	31.0	34.0	31.0	34.0
4	36.38243242091265	37.0	37.0	37.0	35.0	37.0
5	36.24777719999659	37.0	37.0	37.0	35.0	37.0
6	36.28558398049562	37.0	37.0	37.0	35.0	37.0
7	36.26124613194438	37.0	37.0	37.0	35.0	37.0
8	36.252994279966245	37.0	37.0	37.0	35.0	37.0
9	38.05004816421867	39.0	38.0	39.0	35.0	39.0
10	37.992361922135935	39.0	38.0	39.0	35.0	39.0
11	38.06314201198565	39.0	38.0	39.0	35.0	39.0
12	38.021277502621324	39.0	38.0	39.0	35.0	39.0
13	38.04441337686583	39.0	38.0	39.0	35.0	39.0
14	39.43439010459734	40.0	39.0	41.0	37.0	41.0
15	39.44203670710188	40.0	39.0	41.0	37.0	41.0
16	39.44738165667863	40.0	39.0	41.0	37.0	41.0
17	39.417767055674425	40.0	39.0	41.0	37.0	41.0
18	39.41350473543778	40.0	39.0	41.0	37.0	41.0
19	39.43607798341105	40.0	39.0	41.0	37.0	41.0
20	39.420060183961745	40.0	39.0	41.0	37.0	41.0
21	39.40701748403761	40.0	39.0	41.0	37.0	41.0
22	39.36229722011474	40.0	39.0	41.0	36.0	41.0
23	39.31401365647404	40.0	39.0	41.0	36.0	41.0
24	39.261007442011135	40.0	39.0	41.0	36.0	41.0
25	39.21950096754669	40.0	39.0	41.0	36.0	41.0
26	39.1205043177304	40.0	39.0	41.0	36.0	41.0
27	39.04570912221777	40.0	39.0	41.0	36.0	41.0
28	39.003904285336766	40.0	39.0	41.0	35.0	41.0
29	38.93221205895642	40.0	39.0	41.0	35.0	41.0
30	38.86295787975142	40.0	38.0	41.0	35.0	41.0
31	38.808792314184146	40.0	38.0	41.0	35.0	41.0
32	38.71493602257325	40.0	38.0	41.0	35.0	41.0
33	38.65408713887492	40.0	38.0	41.0	35.0	41.0
34	38.565788912852604	40.0	38.0	41.0	35.0	41.0
35	38.54782749537538	40.0	38.0	41.0	35.0	41.0
36	38.51484566138423	40.0	38.0	41.0	35.0	41.0
37	38.472094589410695	40.0	38.0	41.0	34.0	41.0
38	38.41185095518596	40.0	38.0	41.0	34.0	41.0
39	38.359484088758556	40.0	38.0	41.0	34.0	41.0
40	38.29510600390429	40.0	38.0	41.0	34.0	41.0
41	38.24111093114648	40.0	38.0	41.0	34.0	41.0
42	38.19824051420631	40.0	38.0	41.0	34.0	41.0
43	37.347191557196076	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	5.0
20	5.0
21	17.0
22	33.0
23	39.0
24	77.0
25	125.0
26	182.0
27	260.0
28	372.0
29	500.0
30	786.0
31	991.0
32	1426.0
33	1961.0
34	2916.0
35	4403.0
36	6793.0
37	12654.0
38	30891.0
39	52869.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26084547384214	18.082467371938588	12.104989472069015	26.551697682150255
2	20.797565362680828	19.852182734193185	31.969959166972135	27.380292736153855
3	21.072911249968033	21.233174490865846	28.768956669252475	28.924957589913646
4	16.052750475248708	15.287237760747441	33.107998670156086	35.55201309384777
5	16.71426257597586	33.97665953438413	33.073047644215606	16.2360302454244
6	36.23057447552149	32.865046416667376	15.167892794121407	15.73648631368972
7	30.28890006563973	28.44331540317287	20.529891651819586	20.737892879367813
8	28.46547946840342	30.62647582838194	19.420835926244813	21.487208776969833
9	26.923372006785613	13.387095399251535	19.325359952943984	40.36417264101886
10	18.328829481616612	25.23464072902725	32.43455207276633	24.001977716589803
11	37.43766356653908	20.355136522117178	20.305693607372106	21.90150630397163
12	22.92616808886085	24.413717851449615	27.51157219944249	25.148541860247043
13	32.457568602044205	18.809619204310057	22.463280111161314	26.26953208248442
14	24.355750296231257	20.0064787267597	23.070234512859418	32.567536464149626
15	27.01373319580247	26.287433827478324	20.693564748906716	26.005268227812493
16	26.701731354480124	24.459750910005372	22.679805979182827	26.158711756331677
17	25.182640422140196	24.849326979634633	23.82551765879274	26.14251493943243
18	25.31391988542883	23.516073209612383	24.93201599222553	26.237990912733256
19	25.78618496764899	23.845124331881305	24.85188437177662	25.516806328693086
20	26.151039579905717	23.779484600236987	24.406898139070986	25.662577680786313
21	26.723042955663345	23.865583469017192	23.908206671383635	25.503166903935824
22	26.954060712489454	23.5697784445941	23.735156469775887	25.741004373140562
23	26.33517181412874	23.63371324814376	23.89712463876836	26.13399029895914
24	25.764020902418437	24.32335666243276	23.535679882700947	26.37694255244785
25	26.417008362672306	23.61240164696054	23.886895070200413	26.083694920166746
26	26.003563299717836	24.206569087948715	23.961911906365348	25.827955705968105
27	25.907234862369677	23.730041685491916	24.037781206577613	26.324942245560795
28	25.845004986914677	23.555286555789507	24.299487669107556	26.300220788188255
29	25.184345350234853	23.921846096140893	24.771752751327714	26.12205580229654
30	25.140017219773757	24.273913747687693	24.480210047141263	26.10585898539729
31	26.341991526507368	23.904796815194317	24.068469912281447	25.684741746016858
32	25.43582224419685	23.809320841893495	24.388143930029752	26.366712983879903
33	25.119558082637866	23.634565712191087	24.752998542286477	26.492877662884567
34	25.88336586904447	23.21344847281066	24.551817027116883	26.351368631027988
35	25.349723375416644	23.662697025752937	25.181787958092865	25.805791640737553
36	25.044541246472928	23.82466519474541	24.65411271279634	26.476680845985324
37	25.48526515894192	22.49993606519645	25.428150067770893	26.58664870809074
38	24.464865694289344	22.80511819414016	26.295958467951614	26.43405764361888
39	24.548407170927568	22.55193637208351	26.608812773321283	26.290843683667642
40	23.835747227360688	22.174294799116847	27.81931172052819	26.170646252994278
41	23.704467764072053	22.12229449222979	27.782655766493047	26.390581977205112
42	23.141841492835038	22.270623236465	28.281347234180398	26.30618803651956
43	22.875020246021123	21.748915239499773	28.29498665893766	27.08107785554144
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	8.0
2	8.0
3	14.0
4	20.0
5	20.0
6	24.5
7	29.0
8	22.5
9	16.0
10	31.5
11	47.0
12	47.0
13	81.0
14	115.0
15	148.5
16	182.0
17	228.0
18	274.0
19	274.0
20	328.5
21	383.0
22	456.5
23	530.0
24	655.5
25	781.0
26	781.0
27	999.5
28	1218.0
29	1522.5
30	1827.0
31	2189.0
32	2551.0
33	2551.0
34	2947.5
35	3344.0
36	3867.0
37	4390.0
38	4714.5
39	5039.0
40	5039.0
41	5565.5
42	6092.0
43	6501.0
44	6910.0
45	7209.5
46	7509.0
47	7509.0
48	7776.0
49	8043.0
50	8245.0
51	8447.0
52	8723.0
53	8999.0
54	8999.0
55	8988.0
56	8977.0
57	8804.5
58	8632.0
59	8238.0
60	7844.0
61	7844.0
62	7216.5
63	6589.0
64	6160.0
65	5731.0
66	4937.5
67	4144.0
68	4144.0
69	3614.5
70	3085.0
71	2616.0
72	2147.0
73	1747.5
74	1348.0
75	1348.0
76	1071.0
77	794.0
78	652.0
79	510.0
80	423.0
81	336.0
82	336.0
83	276.5
84	217.0
85	161.0
86	105.0
87	77.5
88	50.0
89	50.0
90	31.5
91	13.0
92	14.0
93	15.0
94	9.5
95	4.0
96	4.0
97	3.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	117307.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.3319237315487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.75399641641837	80.93962868150122
2	3.674999859529594	6.345396151727221
3	1.0290037532131617	2.6650762062562805
4	0.5190000096774172	1.7922507700857528
5	0.2979999888708262	1.2863456155599264
6	0.18099999308404027	0.9375646559005324
7	0.12199999533679017	0.7372746004865545
8	0.0899999965599087	0.6215898271079746
9	0.0729999972097035	0.567200717236025
>10	0.24899999048241328	3.4783130741916977
>50	0.009999999617767602	0.6293596999468224
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0017049280946576078	0.0
19	0.0	0.0	0.0	0.0025573921419864117	0.0
20	0.0	0.0	0.0	0.0025573921419864117	0.0
21	0.0	0.0	0.0	0.0034098561893152156	0.0
22	0.0	0.0	0.0	0.005114784283972823	0.0
23	0.0	0.0	0.0	0.005967248331301627	0.0
24	0.0	0.0	0.0	0.007672176425959235	0.0
25	0.0	0.0	0.0	0.011082032615274452	0.0
26	0.0	0.0	0.0	0.013639424757260862	0.0
27	0.0	0.0	0.0	0.04944291474507063	0.0
28	0.0	0.0	0.0	0.11849250257870374	0.0
29	0.0	0.0	0.0	0.20714876350089936	0.0
30	0.0	0.0	0.0	0.3290511222689183	0.0
31	0.0	0.0	0.0	0.5438720621957769	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTGA	25	0.0054764943	29.6	1
ACCAAAC	40	0.0019209235	23.125	8
CACTGTA	45	0.003805668	20.555557	2
GGTATCA	55	5.105355E-4	20.181818	1
TACGCCG	50	0.006998205	18.5	25
CTTATAC	115	1.219034E-6	16.086956	37
GTATCAA	75	0.004075265	14.8	2
TCTTATA	140	9.3849885E-6	13.214286	36
TGTCTCT	600	0.0	12.95	37
TCTCTTA	245	1.4915713E-9	12.081633	37
TATCAAC	135	0.0013590695	10.962962	1
CTCTTAT	170	6.855665E-5	10.882353	35
CTGTCTC	860	0.0	10.755814	37
GTCTCTT	365	5.456968E-12	10.643835	37
ATCAACG	140	0.0018405094	10.571429	2
CAGAGTA	230	1.3731831E-5	9.652174	4
CAACGCA	155	0.0042798817	9.548388	4
TCAACGC	155	0.0042798817	9.548388	3
GCTGTCT	300	3.7499558E-7	9.25	36
AGAGTAC	205	4.5282056E-4	9.024391	5
>>END_MODULE