FastQCFastQC Report
Fri 10 Feb 2017
ERR1632095.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632095.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518794
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.1380123902743671No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5910.11391804839685887No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA400.001929767123.1251
GGTATCA4350.022.9655191
GTGTCTA450.003823021720.5555559
CTTATAC5300.018.537
ATAAGGG609.2275074E-418.53
GCCGTTC1103.839523E-818.58
CGATTTG1002.867382E-718.49999828
ATTGGCG500.00702980818.49999817
CAATACG752.0645131E-417.2666664
GTCTACG650.00157835517.0769231
TACGAAT650.00157835517.0769237
GCGATTT1201.03793354E-716.95833227
TCTTATA9550.016.85340337
GATTTGT1107.787312E-716.81818229
AGCGATT1352.2109816E-816.44444526
GAGCGAT1251.6524427E-716.27999925
AATACGA803.3780927E-416.18755
GTATCAA6200.016.1129042
TTCTATA700.00258992415.8571422
GTGTAGG700.00258992415.8571421
Produced by FastQC (version 0.11.5)