FastQCFastQC Report
Fri 10 Feb 2017
ERR1632094.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632094.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109322
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA200.001834659137.026
TTCCGCG200.001834659137.025
AGAGTAA250.005474877529.610
TCTTCCG358.817935E-426.4285723
CTCTGGA551.88232E-523.5454542
TGCCATC400.001920088223.12500227
GCGGTGG400.001920088223.1250021
CTTTATT400.001920088223.1250022
CTAGATG450.003804030620.5555554
CGTGCCC450.003804030620.5555558
GTGGGTC450.003804030620.55555516
GTGGTTG450.003804030620.55555528
TTGTGCA500.0069952218.521
TTTGTGC500.0069952218.520
GATCACT609.164126E-418.57
TTTATTC500.0069952218.53
GCGTGCC500.0069952218.57
TTTTGTG500.0069952218.519
TCTGGAA500.0069952218.53
TCAGGAT752.0457877E-417.2666663