##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632094.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	109322
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.827198551069316	34.0	31.0	34.0	31.0	34.0
2	32.994054261722255	34.0	33.0	34.0	31.0	34.0
3	33.065046376758566	34.0	33.0	34.0	31.0	34.0
4	36.44690913082454	37.0	37.0	37.0	35.0	37.0
5	36.32582645762061	37.0	37.0	37.0	35.0	37.0
6	36.34791716214486	37.0	37.0	37.0	35.0	37.0
7	36.33721483324491	37.0	37.0	37.0	35.0	37.0
8	36.311053584822815	37.0	37.0	37.0	35.0	37.0
9	38.12644298494356	39.0	39.0	39.0	37.0	39.0
10	38.097702200837894	39.0	38.0	39.0	37.0	39.0
11	38.15937322771263	39.0	39.0	39.0	37.0	39.0
12	38.12596732588134	39.0	38.0	39.0	37.0	39.0
13	38.14568888238415	39.0	39.0	39.0	37.0	39.0
14	39.553374435154865	41.0	39.0	41.0	37.0	41.0
15	39.58446607270266	41.0	39.0	41.0	37.0	41.0
16	39.56251257752328	41.0	39.0	41.0	37.0	41.0
17	39.55263350469256	41.0	39.0	41.0	37.0	41.0
18	39.55217614020965	41.0	39.0	41.0	37.0	41.0
19	39.57230017745742	41.0	39.0	41.0	37.0	41.0
20	39.55407877645854	41.0	39.0	41.0	37.0	41.0
21	39.51513876438411	41.0	39.0	41.0	37.0	41.0
22	39.48319642889812	41.0	39.0	41.0	37.0	41.0
23	39.43972850844295	40.0	39.0	41.0	37.0	41.0
24	39.41021020471634	41.0	39.0	41.0	37.0	41.0
25	39.3407731289219	40.0	39.0	41.0	36.0	41.0
26	39.267978997822944	40.0	39.0	41.0	36.0	41.0
27	39.17983571467774	40.0	39.0	41.0	36.0	41.0
28	39.13001957519987	40.0	39.0	41.0	36.0	41.0
29	39.08036808693584	40.0	39.0	41.0	36.0	41.0
30	39.00782093265765	40.0	39.0	41.0	35.0	41.0
31	38.936636724538516	40.0	38.0	41.0	35.0	41.0
32	38.856195459285416	40.0	38.0	41.0	35.0	41.0
33	38.79507326979016	40.0	38.0	41.0	35.0	41.0
34	38.72184006878762	40.0	38.0	41.0	35.0	41.0
35	38.70632626552752	40.0	38.0	41.0	35.0	41.0
36	38.651268729075575	40.0	38.0	41.0	35.0	41.0
37	38.62374453449443	40.0	38.0	41.0	35.0	41.0
38	38.55428916412067	40.0	38.0	41.0	35.0	41.0
39	38.51475457821847	40.0	38.0	41.0	35.0	41.0
40	38.462514406981214	40.0	38.0	41.0	34.0	41.0
41	38.413430050675984	40.0	38.0	41.0	34.0	41.0
42	38.379356396699656	40.0	38.0	41.0	34.0	41.0
43	37.50652201752621	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	4.0
20	6.0
21	8.0
22	21.0
23	43.0
24	58.0
25	94.0
26	153.0
27	209.0
28	307.0
29	384.0
30	615.0
31	856.0
32	1164.0
33	1691.0
34	2386.0
35	3695.0
36	6084.0
37	10970.0
38	27264.0
39	53306.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.56874188178043	17.62408298421178	12.576608550886373	27.230566583121423
2	19.919137959422624	20.020672874627248	32.831452040760325	27.228737125189806
3	20.697572309324748	20.843928943853935	29.269497447906183	29.18900129891513
4	16.150454620296006	15.19090393516401	32.109730886738255	36.548910557801726
5	16.72216022392565	34.051700481147435	32.60368452827427	16.62245476665264
6	36.57269351091272	32.53142093997548	14.903679039900478	15.992206509211321
7	30.953513473957667	28.2614661275864	19.974936426336875	20.810083972119063
8	27.844349719178208	31.88928120597867	19.590750260697757	20.67561881414537
9	27.019264192019904	14.038345438246647	18.590951501070233	40.351438868663216
10	18.371416549276447	25.95543440478586	31.639560198313237	24.033588847624447
11	37.9127714458206	20.161541135361595	19.984083715994952	21.941603702822853
12	23.283511095662355	23.896379502753334	27.05310916375478	25.767000237829528
13	32.361281352335304	18.12626918644006	22.70174347340883	26.81070598781581
14	24.159821444905873	19.72704487660306	22.692596183750755	33.42053749474031
15	27.444613161120362	25.76242659300049	20.114889958105415	26.678070287773732
16	27.186659592762663	24.40679826567388	21.92513858143832	26.481403560125134
17	25.579480799839004	24.860503832714368	23.090503283876988	26.46951208356964
18	25.52185287499314	23.49115457090064	24.343681967033167	26.64331058707305
19	26.70002378295311	23.87168182067653	23.9658989041547	25.462395492215656
20	26.853698249208758	23.075867620424066	24.222023014580778	25.84841111578639
21	27.011031631327636	23.913759353103675	22.834379173450905	26.24082984211778
22	27.496752712171386	23.308208777739157	22.63405352993908	26.56098498015038
23	26.619527633962058	23.583542196447194	23.03927846179177	26.757651707798978
24	26.84546568851649	23.77655000823256	23.229542086679718	26.148442216571233
25	26.84912460437972	23.255154497722323	23.13623973216736	26.75948116573059
26	27.043961874096706	23.34571266533726	23.055743583176305	26.554581877389726
27	26.728380380893142	23.230456815645525	23.044766835586618	26.99639596787472
28	25.915186330290336	23.289914198423006	23.799418232377747	26.995481238908916
29	25.763341321966298	23.654891055780173	23.939371764146284	26.642395858107243
30	25.733155266094656	24.52205411536562	23.444503393644464	26.300287224895264
31	26.73661294158541	23.56982126196008	23.32924754395273	26.364318252501782
32	25.498984650847955	23.454565412268344	23.971387277949542	27.075062658934158
33	25.771573882658565	23.116115694919596	24.318984284956368	26.793326137465467
34	26.409139971826345	22.87920089277547	23.78386783995902	26.927791295439164
35	25.776147527487602	22.970673789356212	24.56413164779276	26.689047035363423
36	25.548380015001555	23.031960630065313	24.62724794643347	26.79241140849966
37	26.049651488264026	21.723898208960684	25.28676753078063	26.939682771994654
38	24.856844916851138	22.336766616051666	26.070690254477597	26.735698212619603
39	24.859589103748558	21.513510546824975	26.862845538866836	26.764054810559628
40	24.248550154589196	21.548270247525657	27.719031850862592	26.484147747022558
41	23.58720111231042	21.719324564131647	28.19926455791149	26.49420976564644
42	23.73813139166865	21.308611258484113	28.094070726843633	26.859186623003605
43	22.469402316093742	21.32599110883445	28.694132928413314	27.510473646658497
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	2.0
2	0.0
3	4.5
4	9.0
5	9.0
6	11.0
7	13.0
8	15.5
9	18.0
10	22.0
11	26.0
12	26.0
13	46.5
14	67.0
15	102.5
16	138.0
17	154.5
18	171.0
19	171.0
20	216.0
21	261.0
22	309.0
23	357.0
24	460.5
25	564.0
26	564.0
27	696.5
28	829.0
29	1152.0
30	1475.0
31	1682.5
32	1890.0
33	1890.0
34	2236.0
35	2582.0
36	2967.0
37	3352.0
38	3962.5
39	4573.0
40	4573.0
41	5073.0
42	5573.0
43	5697.5
44	5822.0
45	6447.5
46	7073.0
47	7073.0
48	7451.5
49	7830.0
50	8390.0
51	8950.0
52	9572.0
53	10194.0
54	10194.0
55	9434.0
56	8674.0
57	8469.0
58	8264.0
59	7731.0
60	7198.0
61	7198.0
62	6694.0
63	6190.0
64	5629.5
65	5069.0
66	4413.0
67	3757.0
68	3757.0
69	3250.0
70	2743.0
71	2425.0
72	2107.0
73	1739.5
74	1372.0
75	1372.0
76	1126.0
77	880.0
78	730.0
79	580.0
80	451.5
81	323.0
82	323.0
83	263.0
84	203.0
85	153.5
86	104.0
87	77.0
88	50.0
89	50.0
90	38.5
91	27.0
92	16.5
93	6.0
94	4.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	109322.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.23219480068056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.09237065185619	72.0458828049249
2	4.31102016954107	6.745211393863998
3	1.1949722303420052	2.804559009165584
4	0.5752703887752119	1.8001866047090247
5	0.377667348728442	1.4772872797790013
6	0.2502192341420637	1.1745119920967417
7	0.19760304004676996	1.0821243665501912
8	0.15551008477053493	0.9732716196191069
9	0.1169248757673195	0.823256069226688
>10	0.7038877521192634	9.787599934139514
>50	0.022215726395790704	1.0976747589689175
>100	0.00233849751534639	0.18843416695633083
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0018294579316148626	0.0
18	0.0	0.0	0.0	0.0027441868974222936	0.0
19	0.0	0.0	0.0	0.0027441868974222936	0.0
20	0.0	0.0	0.0	0.003658915863229725	0.0
21	0.0	0.0	0.0	0.003658915863229725	0.0
22	0.0	0.0	0.0	0.004573644829037157	0.0
23	0.0	0.0	0.0	0.006403102760652019	0.0
24	0.0	0.0	0.0	0.010976747589689174	0.0
25	0.0	0.0	0.0	0.011891476555496607	0.0
26	0.0	0.0	0.0	0.01646512138453376	0.0
27	0.0	0.0	0.0	0.040248074495526975	0.0
28	0.0	0.0	0.0	0.10793801796527688	0.0
29	0.0	0.0	0.0	0.19575199868279028	0.0
30	0.0	0.0	0.0	0.34393809114359414	0.0
31	0.0	0.0	0.0	0.691535098150418	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	0.0018346591	37.0	26
TTCCGCG	20	0.0018346591	37.0	25
AGAGTAA	25	0.0054748775	29.6	10
TCTTCCG	35	8.817935E-4	26.42857	23
CTCTGGA	55	1.88232E-5	23.545454	2
TGCCATC	40	0.0019200882	23.125002	27
GCGGTGG	40	0.0019200882	23.125002	1
CTTTATT	40	0.0019200882	23.125002	2
CTAGATG	45	0.0038040306	20.555555	4
CGTGCCC	45	0.0038040306	20.555555	8
GTGGGTC	45	0.0038040306	20.555555	16
GTGGTTG	45	0.0038040306	20.555555	28
TTGTGCA	50	0.00699522	18.5	21
TTTGTGC	50	0.00699522	18.5	20
GATCACT	60	9.164126E-4	18.5	7
TTTATTC	50	0.00699522	18.5	3
GCGTGCC	50	0.00699522	18.5	7
TTTTGTG	50	0.00699522	18.5	19
TCTGGAA	50	0.00699522	18.5	3
TCAGGAT	75	2.0457877E-4	17.266666	3
>>END_MODULE