FastQCFastQC Report
Fri 10 Feb 2017
ERR1632093.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632093.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693952
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10310.1485693534999539No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.14626371852808265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7780.1121115005072397No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA250.005494860529.619
GGTATCA4600.022.1195661
TATACAC2300.020.91304436
TGGACTA555.1402726E-420.1818185
CAGTATG555.1402726E-420.1818184
TATATTG500.007032140618.55
GTATAGA500.007032140618.51
CCTTTAG953.602223E-617.5263161
ACAGCGC752.0657794E-417.2666668
TCTAGCG650.001579080917.07692328
GAGCGTC650.001579080917.0769237
TAACGCC1107.7966433E-716.8181824
TCTTATA10200.016.68627437
CTTATAC5550.016.33333437
GATCAAG1059.332351E-615.8571421
ATCTCGC1455.3341864E-815.31034511
CGACTTT1353.9652514E-715.07407535
ATACACA3100.014.91935437
ATCCTAA750.00410222714.81
CTAGCAC1001.09237204E-414.83
Produced by FastQC (version 0.11.5)